Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA17691, author = {Windi Susmayanti and Enny Fachriyah and Dewi Kusrini}, title = {Isolasi, Identifikasi dan Uji Sitotoksik Senyawa Flavonoid dari Ekstrak Etil Asetat Daun Binahong (Anredera Cordiforlia (Tenns) Stennis)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {15}, number = {3}, year = {2012}, keywords = {pemisahan; binahong; Anredera cordifolia (Ten). Steenis.; Auron}, abstract = {Telah dilakukan isolasi, identifikasi dan uji sitotoksik senyawa flavonoid dari ekstrak etil asetat daun binahong ( Anredera cordiforlia (Tenns) Stennis). Pemisahan dilakukan dengan metode kromatografi lapis tipis (KLT), Kromatografi kolom, KLT Preparatif. Pengujian kemurnian dilakukan KLT dan KLT 2 dimensi. Analisis dilakukan dengan analisis spektrometri UV-Vis dengan pereaksi geser, sedangkan pengujian sitotoksik menggunakan BSLT (Brine Shrimp Lethality Test). Ekstrak etil asetat dan fraksi B hasil kolom mengandung senyawa flavonoid. Analisis isolat menggunakan spektrometri UV-Vis dengan pereaksi geser menunjukkan panjang gelombang 377nm (pita I) dan 438 nm (pita II) merupakan golongan auron dan senyawa yang terkandung adalah 4’,6,7 trihidroksi Auron. Uji sitotoksik menunjukkan hasil ekstrak n-heksana, ekstrak etanol, ekstrak etil asetat tidak bersifat toksik dengan masing-masing LC 50 591,68 ppm , LC 50 1479,151 ppm LC 50 611,706 ppm, dan hasil fraksi B kolom bersifat sedikit toksik dengan LC 50 58,447 ppm.}, issn = {2597-9914}, pages = {88--93} doi = {10.14710/jksa.15.3.88-93}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/17691} }
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