Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA18460, author = {Edwin Fadhly and Dewi Kusrini and Enny Fachriyah}, title = {Isolasi, Identifikasi Senyawa Alkaloid dari Daun Rivina humilis L. serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {18}, number = {2}, year = {2015}, keywords = {Alkaloids; Rivina humilis L.; BSLT}, abstract = {Penelitian tentang penapisan fitokimia, isolasi, identifikasi alkaloid dari daun getih-getihan ( Rivina humilis L.) serta uji sitotoksisitas dengan metode BSLT telah dilakukan. Isolasi alkaloid diawali dengan maserasi daun Rivina humilis L. dengan pelarut etanol 96%, dilanjutkan penghilangan klorofil dengan menggunakan aquades (1:1). Selanjutnya hasil maserasi tersebut dipartisi dengan pelarut n-heksana, diperoleh ekstrak n-heksana dan ekstrak etanol-air. Ekstrak etanol-air dilakukan isolasi alkaloid hingga diperoleh ekstrak alkaloid berwarna coklat kemerahan sebanyak 0,7323 gram. Pemisahan alkaloid dilakukan dengan metode kromatografi lapis tipis preparatif dan uji kemurniannya menggunakan metode KLT dengan berbagai eluen didapatkan 1 noda pada pita A1. Isolat alkaloid A1 berbentuk padatan putih dan mempunyai titik leleh sebesar 290-292°C. Hasil analisis spektrofotometer UV-Vis memperkirakan bahwa senyawa alkaloid A1 mempunyai struktur dasar indol. Analisis dengan spektofotometer FTIR menunjukkan adanya gugus N-H, O-H, =C-H aromatik, CH2, C=N, C=O, C=C aromatik, dan C-O eter. Sedangkan kromatogram LC-MS menunjukkan puncak tertinggi pada waktu retensi 1,8 menit dan memiliki bobot molekul sebesar 267.27 g/mol. Hasil uji sitotoksik menunjukkan bahwa ekstrak alkaloid mempunyai harga LC 50 sebesar 25,439 ppm.}, issn = {2597-9914}, pages = {67--72} doi = {10.14710/jksa.18.2.67-72}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/18460} }
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