Uji Sitotoksik Ekstrak dan Fraksi Dendrophtoe pentandra (L) Miq pada sel T47D

Masayu Farah Diba; Salni Salni; Subandrate Subandrate

doi:10.14710/jksa.22.3.73-78

DOI: https://doi.org/10.14710/jksa.22.3.73-78

Uji Sitotoksik Ekstrak dan Fraksi Dendrophtoe pentandra (L) Miq pada sel T47D

Cytotoxic Test of Extract and Fraction of Dendrophtoe Pentandra (L) Miq on T47D Cell

Masayu Farah Diba¹, Salni Salni²

, Subandrate Subandrate³

¹Biomedical Program, Faculty of Medicine, Universitas Sriwijaya, Indonesia

²Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Sriwijaya, Indonesia

³Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya, Indonesia

Received: 14 Jan 2019; Revised: 8 Apr 2019; Accepted: 24 Apr 2019; Available online: 27 May 2019; Published: 31 May 2019.

BibTex Citation Data :

@article{JKSA22075,
    author = {Masayu Diba and Salni Salni and Subandrate Subandrate},
    title = {Uji Sitotoksik Ekstrak dan Fraksi Dendrophtoe pentandra (L) Miq pada sel T47D},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {22},
    number = {3},
    year = {2019},
    keywords = {Cytotoxic; Dendrophtoe petandra (L.) Miq; Extract; Fraction},
    abstract = {Indonesia has a variety of plants that have the potential for medicine. One of the plants used by the community as a drug with anticancer effects is  Dendrophtoe petandra  (L.) Miq. This study aims to determine the cytotoxic effect of ethanol extract, ethyl acetate fraction and water ethanol fraction of  Dendrophtoe petandra  (L.) Miq on T47D breast cancer cells. The cytotoxic effects of ethanol extract, ethyl acetate fraction and water ethanol fraction were carried out by MTT assay method using series concentration. Cytotoxic effects were assessed by calculating IC 50  values using linear equations. Phytochemical test showed that  Dendrophtoe petandra  (L.) Miq contained saponins, terpenoids, flavonoids and tannins. The IC 50  value of the ethanol extract, ethyl acetate fraction and water ethanol fraction were 417.506 μg/mL, 233.617 μg/mL, and 2748.357 μg/mL, respectively. The smaller the IC 50  value means that the compound is more active. Water ethanol fraction of  Dendrophtoe petandra  (L.) Miq does not have a cytotoxic effect, whereas ethanol extract and ethyl acetate fraction of  Dendrophtoe petandra  (L.) Miq have cytotoxic effects in the medium strength category. The content of flavonoids and saponins in ethanol extract and ethyl acetate fraction of  Dendrophtoe petandra  (L.) Miq is thought to play role in causing T47D cell death. Cytotoxic effects of ethyl acetate fraction are stronger than ethanol extracts.},
   issn = {2597-9914},   pages = {73--78}  doi = {10.14710/jksa.22.3.73-78},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/22075}
}

Citation Format:

Abstract

Indonesia has a variety of plants that have the potential for medicine. One of the plants used by the community as a drug with anticancer effects is Dendrophtoe petandra (L.) Miq. This study aims to determine the cytotoxic effect of ethanol extract, ethyl acetate fraction and water ethanol fraction of Dendrophtoe petandra (L.) Miq on T47D breast cancer cells. The cytotoxic effects of ethanol extract, ethyl acetate fraction and water ethanol fraction were carried out by MTT assay method using series concentration. Cytotoxic effects were assessed by calculating IC₅₀ values using linear equations. Phytochemical test showed that Dendrophtoe petandra (L.) Miq contained saponins, terpenoids, flavonoids and tannins. The IC₅₀ value of the ethanol extract, ethyl acetate fraction and water ethanol fraction were 417.506 μg/mL, 233.617 μg/mL, and 2748.357 μg/mL, respectively. The smaller the IC₅₀value means that the compound is more active. Water ethanol fraction of Dendrophtoe petandra (L.) Miq does not have a cytotoxic effect, whereas ethanol extract and ethyl acetate fraction of Dendrophtoe petandra (L.) Miq have cytotoxic effects in the medium strength category. The content of flavonoids and saponins in ethanol extract and ethyl acetate fraction of Dendrophtoe petandra (L.) Miq is thought to play role in causing T47D cell death. Cytotoxic effects of ethyl acetate fraction are stronger than ethanol extracts.

Fulltext View|Download

Keywords: Cytotoxic; Dendrophtoe petandra (L.) Miq; Extract; Fraction

Article Metrics:

Article Info

Section: Research Articles

Language : ID

In Vol 22, No 3 (2019): Volume 22 Issue 3 Year 2019

Recent articles

Interlayer-free Silica Pectin Membrane for Wetland Saline Water via Pervaporation Removal of natural organic matter for wetland saline water desalination by coagulation-pervaporation Isolation of Flavonoid Compounds from Amyl Alcohol Fraction of Golek Mango Leaf (Mangifera indica L cv. Golek) More recent articles

Most cited articles

Sintesis Asam Eugenoksi Asetat (EOA) dari Eugenol untuk Ekstraktan Logam Berat dan Recovery Krom dari Limbah Elektroplating Asam Anakardat dari Kulit Biji Jambu Mete (Anacardium Occidentale L) yang Mempunyai Aktivitas Sitotoksik Preparasi Katalis Zeolit Alam Asam sebagai Katalis dalam Proses Pirolisis Katalitik Polietilena Pirolisis Kulit Biji Jambu Mete (Anacardium occidentale L.) dengan Katalis Ni-Ag/Zeolit Konsentrasi Hambat Minimum (KHM) Kadar Sampel Alang-Alang (Imperata cylindrica) dalam Etanol Melalui Metode Difusi Cakram More cited articles

Soheil Zorofchian Moghadamtousi, Muhamad Noor Alfarizal Kamarudin, Chim Kei Chan, Bey Hing Goh, Habsah Abdul Kadir, Phytochemistry and Biology of Loranthus parasiticus Merr, a Commonly Used Herbal Medicine, The American Journal of Chinese Medicine, 42, 01, (2014) 23-35 https://doi.org/10.1142/S0192415X14500025
Kementerian Kesehatan Republik Indonesia, Stop Kanker, in: InfoDATIN, Jakarta, 2015
Agustina Tri Endharti, Adisti Wulandari, Anik Listyana, Eviana Norahmawati, Sofy Permana, Dendrophthoe pentandra (L.) Miq extract effectively inhibits inflammation, proliferation and induces p53 expression on colitis-associated colon cancer, BMC Complementary and Alternative Medicine, 16, 1, (2016) 374 https://doi.org/10.1186/s12906-016-1345-0
Vida Elsyana, Maria Bintang, Bambang Pontjo Priosoeryanto, Cytotoxicity and Antiproliferative Activity Assay of Clove Mistletoe (Dendrophthoe pentandra (L.) Miq.) Leaves Extracts, Advances in Pharmacological Sciences, 2016, (2016) 6 https://doi.org/10.1155/2016/3242698
Hermansyah Amir, Bambang Gonggo Murcitro, Uji Microtetrazolium (Mtt) Ekstrak Metanol Daun Phaleria macrocarpa (Scheff.) Boerl terhadap Sel Kanker Payudara Mcf-7, ALOTROP Jurnal Pendidikan dan Ilmu Kimia, 1, 1, (2017) 27-32
Azis Saifudin, Senyawa Alam Metabolit Sekunder Teori, Konsep, dan Teknik Pemurnian, Deepublish, Yogyakarta, 2014
P.D. Padmasari, K.W. Astuti, N.K. Warditiani, Skrining Fitokimia Ekstrak Etanol 70% Rimpang Bangle (Zingiber purpureum Roxb.), Jurnal Farmasi Udayana, 2, 4, (2013) 1-4
Maisarah Abdul Mutalib, Faisal Ali, Fauziah Othman, Rajesh Ramasamy, Asmah Rahmat, Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells, SpringerPlus, 5, 1, (2016) 2105 https://doi.org/10.1186/s40064-016-3777-x
Lau Shin Yee, Fauzi, Nurul Fatihah Mohd, Nik Nurul Najihah, Nik Mat Daud, Mohd Dasuki Sulain, Study of Dendrophthoe Pentandra Ethyl Acetate Extract as Potential Anticancer Candidate on Safety and Toxicity Aspects, Journal of Analytical & Pharmaceutical Research, 6, 1, (2017) 00167 http://doi.org/10.15406/japlr.2017.06.00167
Rizky Maulida Amalia Hanif, Rudi Kartika, Partomuan Simanjuntak, Isolasi dan Identifikasi Senyawa Kimia dari Ekstrak n-heksan Batang Benalu Tanaman Jeruk (Dendrophtoe pentandra (L.) Miq.), Jurnal Kimia Mulawarman, 14, 1, (2016) 36-41
Ardy Prian Nirwana, Aktivitas Antiproliferasi Ekstrak Etanol Daun Benalu Kersen (Dendrophtoe pentandra L. Miq.) terhadap Kultur Sel Kanker Nasofaring (Raji Cell Line), Study program of Bioscience, Universitas Sebelas Maret,
Heri Wijaya, Novitasari Novitasari, Siti Jubaidah, Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambai Laut (Sonneratia Caseolaris L. Engl), Jurnal Ilmiah Manuntung, 4, 1, (2018) 79-83
Liza Pratiwi, Achmad Fudholi, Ronny Martien, Suwidjiyo Pramono, Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers, JPSCR : Journal of Pharmaceutical Science and Clinical Research, 1, 1, (2016) 71-82 http://doi.org/10.20961/jpscr.v1i2.1936
Suprapto Ma’at, Tanaman Obat untuk Pengobatan Kanker, Jurnal Bahan Alam Indonesia, 2, 4, (2003) 146-150
Mohsen Marvibaigi, Eko Supriyanto, Neda Amini, Fadzilah Adibah Abdul Majid, Saravana Kumar Jaganathan, Preclinical and Clinical Effects of Mistletoe against Breast Cancer, BioMed Research International, 2014, (2014) 15 https://doi.org/10.1155/2014/785479
Nina Artanti, Taufik Firmansyah, Akhmad Darmawan, Bioactivities evaluation of Indonesian mistletoes (Dendrophthoe pentandra (L.) Miq.) leaves extracts, Journal of Applied Pharmaceutical Science, 2, 1, (2012) 24
Palak Mayur Shah, Vishnu V Priya, R Gayathri, Quercetin-a flavonoid: a systematic review, Journal of Pharmaceutical Sciences and Research, 8, 8, (2016) 878
Tiana Fitrilia, Maria Bintang, Mega Safithri, Phytochemical screening and antioxidant activity of clove mistletoe leaf extracts (Dendrophthoe pentandra (L.) Miq), IOSR Journal of Pharmacy, 5, 8, (2015) 13-18
Pangkalan Ide, Dark Chocolate Healing, Elex Media Komputindo, 2013
Muthi Ikawati, Andy Wibowo, Navista Sri Octa U, Rosa Adelina, Pemanfaatan Benalu sebagai Agen Antikanker, in: International Seminar of Indonesia – Malaysia Update 2008, Yogyakarta, Indonesia, 2008
Maya Fitria, DB Septhea, AHM Ikawati, E Meiyanto, Ekstrak Etanolik Herba Ciplukan (Physalis angulata L.) Berefek Sitotoksik dan Menginduksi Apoptosis pada Sel kanker Payudara MCF-7, Bionatura, 13, 2, (2011) 101-107
Dita Widia Ningrum, Dewi Kusrini, Enny Fachriyah, Uji Aktivitas Antioksidan Senyawa Flavonoid dari Ekstrak Etanol Daun Johar (Senna siamea Lamk), Jurnal Kimia Sains dan Aplikasi, 20, 3, (2017) 123-129 https://doi.org/10.14710/jksa.20.3.123-129

Last update:

Inhibitory Activity of HEp-2 Cells by Honey from Indonesia
La Ode Sumarlin, Anna Muawanah, Farhan Riza Afandi, Adawiah Adawiah. Jurnal Kimia Sains dan Aplikasi, 22 (6), 2019. doi: 10.14710/jksa.22.6.317-325

Last update: 2024-04-20 04:24:12

No citation recorded.

As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.

Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).

Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.

The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.

Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners

JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.

The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.

Adi Darmawan, Ph.D (Editor in Chief)

Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jl. Prof. Soedarto, Kampus Undip Tembalang, Semarang, Central Java, Indonesia 50275
Email: jksa@live.undip.ac.id