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Isolasi Senyawa Flavonoid dari Fraksi Amil Alkohol Daun Mangga Golek (Mangifera indica L. cv. Golek)

Isolation of Flavonoid Compounds from Amyl Alcohol Fraction of Golek Mango Leaf (Mangifera indica L cv. Golek)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Received: 12 Jan 2019; Revised: 15 Apr 2019; Accepted: 24 Apr 2019; Available online: 26 May 2019; Published: 31 May 2019.
Open Access Copyright 2019 Jurnal Kimia Sains dan Aplikasi under

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Mango plant (Mangifera indica L.) is a tropical fruit plant with high nutrition in the Anacardiaceae family. One of the several varieties of mango plants spread throughout Indonesia, namely mango golek. This study aims to obtain flavonoid isolates and find out the types of flavonoids in the leaves of mango golek. In this study, maceration was carried out to obtain ethanol extract, then followed by phytochemical tests, removal of chlorophyll using distilled water (1: 1), hydrolysis using 2N HCl, followed by liquid-liquid extraction using ethyl acetate and amyl alcohol. In the amyl alcohol fraction, flavonoid was separated using gravity column chromatography with a mixture of eluent n-butanol:n-hexane:methanol (4:2:1). Phytochemical tests showed positive results for alkaloids, saponins, tannins, quinones and flavonoids. UV-Vis spectrophotometer analysis provides absorption at a wavelength of 263 nm while FTIR analysis showed the presence of O-H, C=O, C-O-C aliphatic, and C-O alcohol groups. LC-MS/MS instrument analysis showed m/z of 300 which is the molecular mass of isoflavone compound.

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Keywords: mango golek; flavonoids; isoflavones; FTIR; UV-Vis spectrophotometer

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  1. Bárbara B. Garrido-Suárez, Gabino Garrido, Rene Delgado, Fe Bosch, María del C. Rabí, A Mangifera indica L. Extract Could Be Used to Treat Neuropathic Pain and Implication of Mangiferin, Molecules, 15, 12, (2010) 9035-9045
  2. Juliana Aparecida Severi, Zeila Pinheiro Lima, Hélio Kushima, Alba Regina Monteiro Souza Brito, Lourdes Campaner dos Santos, Wagner Vilegas, Clélia Akiko Hiruma-Lima, Polyphenols with Antiulcerogenic Action from Aqueous Decoction of Mango Leaves (Mangifera indica L.), Molecules, 14, 3, (2009) 1098-1110
  3. Qudsia Kanwal, Ishtiaq Hussain, Hamid Latif Siddiqui, Arshad Javaid, Antifungal activity of flavonoids isolated from mango (Mangifera indica L.) leaves, Natural Product Research, 24, 20, (2010) 1907-1914
  4. M. T. Yakubu, S. S. Salimon, Antidiarrhoeal activity of aqueous extract of Mangifera indica L. leaves in female albino rats, Journal of Ethnopharmacology, 163, (2015) 135-141
  5. Antony V. Samrot, Bennet Rohan, Divya Kumar, K. Sahiti, P. Raji, K. Sree Samanvitha, Detection of antioxidant and antibacterial activity of Mangifera indica using TLC bio-autography, International Journal of Pharmaceutical Sciences and Research, 7, 11, (2016) 4467-4472
  6. Pintu K. De, Arna Pal, Effects of aqueous young leaves extract of Mangifera indica on gm (–) microorganisms causing gastro-intestinal disorders, Asian Journal of Plant Science and Research, 4, 1, (2014) 23-27
  7. Kalpna Rakholiya, Sumitra Chanda, Pharmacognostic, Physicochemical and Phytochemical Investigation of Mangifera indica L. var. Kesar leaf, Asian Pacific Journal of Tropical Biomedicine, 2, 2, Supplement, (2012) S680-S684
  8. C. G. Mohan, M. Deepak, G. L. Viswanatha, G. Savinay, V. Hanumantharaju, C. E. Rajendra, Praveen D. Halemani, Anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica, Asian Pacific Journal of Tropical Medicine, 6, 4, (2013) 311-314
  9. María Fernández Ponce, Lourdes Casas Cardoso, C. Mantell, Enrique Martinez de la Ossa, Potential Use of Mango Leaves Extracts Obtained by High Pressure Technologies in Cosmetic, Pharmaceutics and Food Industries, Chemical Engineering Transactions, 32, (2013) 1147-1152
  10. Tang-Bin Zou, En-Qin Xia, Tai-Ping He, Ming-Yuan Huang, Qing Jia, Hua-Wen Li, Ultrasound-Assisted Extraction of Mangiferin from Mango (Mangifera indica L.) Leaves Using Response Surface Methodology, Molecules, 19, 2, (2014) 1411-1421
  11. Abdelnaser Abdelghany Elzaawely, Shinkichi Tawata, Preliminary phytochemical investigation on mango (Mangifera indica L.) leaves, World Journal of Agricultural Sciences, 6, 6, (2010) 735-739
  12. Irda Fidrianny, Ari Sri Windyaswari, Komar Ruslan Wirasutisna, Antioxidant Capacities of Various Leaves Extract from Five Colors Varieties of Sweet Potatoes Tubers Using ABTS, DPPH Assays and Correlation with Total Flavonoid, Phenolic, Carotenoid Content, Research Journal of Medicinal Plants, 7, 3, (2013) 130-140
  13. Qudsia Kanwal, Hussain Ishtiaq, Hamid Latif Siddiqui, Arshad Javaid, Flavonoids from mango leaves with antibacterial activity, Journal of the Serbian Chemical Society, 74, 12, (2009) 1389-1399
  14. Enny Fachriyah, Dewi Kusrini, Pratama Jujur Wibawa, Improvement of Bioactivity with Nanoparticle Fabrication: Cytotoxic Test of Ethanol, N-Hexane and Ethyl Acetate Extract from Red Galangal Rhizome (Alpinia purpurata (Vieill.) K. Schum) in Bulk and Nanoparticle size using BSLT Method, Jurnal Kimia Sains dan Aplikasi, 21, 1, (2018) 39-43
  15. Norman R. Farnsworth, Biological and phytochemical screening of plants, Journal of Pharmaceutical Sciences, 55, 3, (1966) 225-276
  16. K. R. Markham, Cara Mengidentifikasi Flavonoid, K. Padmawinata, ITB, Bandung, 1988
  17. Kautsar Elvira, Enny Fachriyah, Dewi Kusrini, Isolation of Flavonoid Compounds from Eceng Gondok (Eichhornia crassipes) and Antioxidant Tests with DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Method, Jurnal Kimia Sains dan Aplikasi, 21, 4, (2018) 187-192
  18. Windi Susmayanti, Enny Fachriyah, Dewi Kusrini, Isolasi, Identifikasi dan Uji Sitotoksik Senyawa Flavonoid dari Ekstrak Etil Asetat Daun Binahong (Anredera Cordiforlia (Tenns) Stennis), Jurnal Kimia Sains dan Aplikasi, 15, 3, (2012) 88-93
  19. C. Sandeep Kumar Reddy, K. K. Azam Khan, C. Nagaraja, A Review on the Determination of Melting Point Measurement System, International Journal of Advanced Research in Electrical, Electronics and Instrumentation Engineering, 5, 2, (2016) 975-979
  20. Tom Mabry, K.R. Markham, M.B. Thomas, The Systematic Identification of Flavonoids, Springer Berlin Heidelberg, 1970
  21. Robert Milton Silverstein, Francis X. Webster, David J. Kiemle, Spectrometric Identification of Organic Compounds, 7 ed., John Wiley & Sons, 2005

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