DOI: https://doi.org/10.14710/jksa.22.5.179-183

Antioxidant Activity of Daemonorops draco Resin

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Bogor, Indonesia

3Tropical Biopharmaca Research Center, IPB University,Bogor, Indonesia

Received: 6 May 2019; Revised: 5 Aug 2019; Accepted: 13 Sep 2019; Published: 30 Sep 2019.
Open Access Copyright 2019 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

Fulltext View|Download
Keywords: Antioxidant; chromatography; Daemonorops draco; fractionation; resin
Funding: Ministry of Research, Technology and Higher Education in accordance with the Letter of Appointment Implementation of Research Program Number: 079/SP2H/LT/DRPM/ II/2016 17 February 2016

Article Metrics:

Article Info
Section: Research Articles
Language : EN
Recent articles
Synthesis of Silver Nanoparticles (NPAg) using Andaliman (Zanthoxylum acanthopodium DC.) Fruit Water Extract and Its Application in Indigosol Blue Photodegradation Antioxidant Activity of Daemonorops draco Resin A Study on Photo-Fenton Method for Simulatenous and Synergic Decreasing Concentration of Pb(II) and Cu(II) in the Solution More recent articles
Most cited articles
Biolubrication Synthesis Made from Used Cooking Oil and Bayah Natural Zeolite Catalyst Modifikasi Zeolit Alam dengan Ligan EDTA untuk Adsorpsi Ion Logam Pb2+ dan Cd2+ Pemanfaatan Karbon Aktif Serbuk Gergaji Kayu Jati untuk Menurunkan Chemical Oxygen Demand (COD) Limbah Cair Industri Tekstil Molecular Docking of Interaction between E-Cadherin Protein and Conformational Structure of Cyclic Peptide ADTC3 (Ac-CADTPC-NH2) Simulated on 20 ns Pengaruh Pemerangkapan Enzim Alkalin Fosfatase ke dalam Silika dari Abu Sekam Padi terhadap Aktivitas Enzimatiknya More cited articles
  1. Dong-Hoon Hyun, Joe O. Hernandez, Mark P. Mattson and Rafael de Cabo, The plasma membrane redox system in aging, Ageing Research Reviews, 5, 2, (2006) 209-220 https://doi.org/10.1016/j.arr.2006.03.005
  2. Vuokko L. Kinnula and James D. Crapo, Superoxide dismutases in malignant cells and human tumors, Free Radical Biology and Medicine, 36, 6, (2004) 718-744 https://doi.org/10.1016/j.freeradbiomed.2003.12.010
  3. Uma Singh and Ishwarlal Jialal, Oxidative stress and atherosclerosis, Pathophysiology, 13, 3, (2006) 129-142 https://doi.org/10.1016/j.pathophys.2006.05.002
  4. Mark A. Smith, Catherine A. Rottkamp, Akihiko Nunomura, Arun K. Raina and George Perry, Oxidative stress in Alzheimer’s disease, Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1502, 1, (2000) 139-144 https://doi.org/10.1016/S0925-4439(00)00040-5
  5. Termentzi Aikaterini, Fokialakis Nikolas and Skaltsounis Alexios Leandros, Natural Resins and Bioactive Natural Products thereof as Potential Anitimicrobial Agents, Current Pharmaceutical Design, 17, 13, (2011) 1267-1290 http://dx.doi.org/10.2174/138161211795703807
  6. Mardho Tillah, Irmanida Batubara and Rita Kartika Sari, Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia), Biosaintifika: Journal of Biology & Biology Education, 9, 1, (2017) 134-139 https://doi.org/10.15294/biosaintifika.v9i1.8371
  7. Deepika Gupta and Rajinder K. Gupta, Bioprotective properties of Dragon's blood resin: In vitro evaluation of antioxidant activity and antimicrobial activity, BMC Complementary and Alternative Medicine, 11, 1, (2011) 1-9 https://doi.org/10.1186/1472-6882-11-13
  8. Deepika Gupta, Bruce Bleakley and Rajinder K. Gupta, Dragon's blood: Botany, chemistry and therapeutic uses, Journal of Ethnopharmacology, 115, 3, (2008) 361-380 https://doi.org/10.1016/j.jep.2007.10.018
  9. Rana Rio Andhika, Bambang Hariyadi and Fachruddin Saudagar, Etnobotani Penghasil Getah oleh Suku Anak Dalam di Taman Nasional Bukit Duabelas Kabupaten Sarolangun, Jambi, Jurnal Ilmu Pertanian Indonesia, 20, 1, (2015) 33-38
  10. G. S. R. Rao, M. A. Gerhart, R. T. Lee, L. A. Mitscher and S. Drake, Antimicrobial Agents From Higher Plants. Dragon's Blood Resin, Journal of Natural Products, 45, 5, (1982) 646-648 http://doi.org/10.1021/np50023a024
  11. Wulan Tri Wahyuni, Sri Purwanti and Irmanida Batubara, Antibacterial and Antibiofilm Activity of Daemonorops draco Resin, Biosaintifika: Journal of Biology & Biology Education, 10, 1, (2018) 138-144 https://doi.org/10.15294/biosaintifika.v10i1.13554
  12. Jing-hua Yu, Gui-bin Zheng, Chun-yu Liu, Li-ying Zhang, Hong-mei Gao, Ya-hong Zhang, Chun-yan Dai, Lin Huang, Xian-ying Meng, Wen-yan Zhang and Xiao-fang Yu, Dracorhodin Perchlorate Induced Human Breast Cancer MCF-7 Apoptosis through Mitochondrial Pathways, International Journal of Medical Science, 10, 9, (2013) 1149-1156 http://doi.org/10.7150/ijms.6275
  13. Ping-Chung Kuo, Hsin-Yi Hung, Tsong-Long Hwang, Wen-Ke Du, Hsiang-Chih Ku, E. Jian Lee, Shih-Huang Tai, Fu-An Chen and Tian-Shung Wu, Anti-inflammatory Flavan-3-ol-dihydroretrochalcones from Daemonorops draco, Journal of Natural Products, 80, 4, (2017) 783-789 http://doi.org/10.1021/acs.jnatprod.7b00039
  14. Qian Hao, Yoshinori Saito, Yosuke Matsuo, Hai-Zhou Li and Tanaka Takashi, Three new flavans in dragon's blood from Daemonorops draco, Natural Product Research, 29, 15, (2015) 1419-1425 http://doi.org/10.1080/14786419.2014.1003137
  15. Alberto Arnone, Gianluca Nasini, Orso Vajna de Pava and Lucio Merlini, Constituents of Dragon's Blood. 5. Dracoflavans B1, B2, C1, C2, D1, and D2, New A-Type Deoxyproanthocyanidins, Journal of Natural Products, 60, 10, (1997) 971-975 http://doi.org/10.1021/np9702188
  16. Ken-ichi Nakashima, Naohito Abe, Fumiko Kamiya, Tetsuro Ito, Masayoshi Oyama and Munekazu Iinuma, Novel Flavonoids in Dragon's Blood of Daemonorops draco, Helvetica Chimica Acta, 92, 10, (2009) 1999-2008 http://doi.org/10.1002/hlca.200900086
  17. Irmanida Batubara, Tohru Mitsunaga and Hideo Ohashi, Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities, Journal of Wood Science, 55, 3, (2009) 230-235 http://doi.org/10.1007/s10086-008-1021-1
  18. Bishnu Prasad Pandey, Rupak Thapa and Anil Upreti, Chemical composition, antioxidant and antibacterial activities of essential oil and methanol extract of Artemisia vulgaris and Gaultheria fragrantissima collected from Nepal, Asian Pacific Journal of Tropical Medicine, 10, 10, (2017) 952-959 https://doi.org/10.1016/j.apjtm.2017.09.005

Last update:

  1. Advances in the Study of Chemical Constituents and Pharmacological Effects of Sanguis Draconis

    Ying Zhang, Jun-wen Tao, Miao-miao Guo, Dan Yu, Li Li. Asian Journal of Beauty and Cosmetology, 21 (3), 2023. doi: 10.20402/ajbc.2023.0014

  2. Antioxidant Activity, Sun Protection Activity, and Phytochemical Profile of Ethanolic Extracts of Daemonorops acehensis Resin and Its Phytosomes

    Rita Kartika Sari, Yanico Hadi Prayogo, Salman Arib Rozan, Mohamad Rafi, Ietje Wientarsih. Scientia Pharmaceutica, 90 (1), 2022. doi: 10.3390/scipharm90010010

  3. Intermittent drying of dragon blood’s resin

    K Yuniarti, L Efiyanti, E Basri, G Pasaribu, TK Waluyo. IOP Conference Series: Earth and Environmental Science, 1034 (1), 2022. doi: 10.1088/1755-1315/1034/1/012010

  4. Retro-dihydrochalcones from the resin of Daemonorops draco and their inhibitory activities against renal fibrosis

    Bai-Liang Zhang, Cheng-Tian Tao, Lan-Sheng Zhang, Yong-Xian Cheng. Fitoterapia, 168 , 2023. doi: 10.1016/j.fitote.2023.105515

  5. Anti-inflammatory, pro-proliferative and antimicrobial potential of the compounds isolated from Daemonorops draco (Willd.) Blume

    L. Apaza Ticona, Á. Rumbero Sánchez, J. Sánchez Sánchez-Corral, P. Iglesias Moreno, M. Ortega Domenech. Journal of Ethnopharmacology, 268 , 2021. doi: 10.1016/j.jep.2020.113668

  6. Phytochemical profiling of Daemonorops acehensis Rustiami with different solvent extraction using LC-MS/MS and correlation with their antioxidant activity

    Cahya Gioktavian, Mohamad Rafi, Rita Kartika Sari, Wulan Tri Wahyuni. Journal of Herbs, Spices & Medicinal Plants, 30 (2), 2024. doi: 10.1080/10496475.2023.2301698

  7. Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne

    Shafira Ananda Djakaria, Irmanida Batubara, Rika Raffiudin. Jurnal Kimia Sains dan Aplikasi, 23 (8), 2020. doi: 10.14710/jksa.23.8.267-275

  8. In vitro and in vivo studies of Dragon's blood plant (D. cinnabari)-loaded electrospun chitosan/PCL nanofibers: Cytotoxicity, antibacterial, and wound healing activities

    Mohammad Irani, Parvaneh GhaderiShikhi Abadi, Mohammad Mahdi Ahmadian-Attari, Aryan Rezaee, Hananeh Kordbacheh, Pouya Goleij. International Journal of Biological Macromolecules, 257 , 2024. doi: 10.1016/j.ijbiomac.2023.128634

  9. Dragon’s blood extracted from used rattan fruit (Daemonorops draco): Resin yield and its physical properties

    Mahlinda, Lancy Maurina, Ridho Kurniawan. 3RD INTERNATIONAL CONFERENCE ON CHEMISTRY, CHEMICAL PROCESS AND ENGINEERING (IC3PE), 2370 , 2021. doi: 10.1063/5.0062167

  10. In-vitro germination and propagation to support ex-situ conservation of Calamus draco Willd. in Aceh

    Yelnititis, Vivi Yuskiantiand, Asri Insiana Putri. PROCEEDINGS OF THE 9TH INTERNATIONAL SYMPOSIUM ON INNOVATIVE BIOPRODUCTION INDONESIA ON BIOTECHNOLOGY AND BIOENGINEERING 2022: Strengthening Bioeconomy through Applied Biotechnology, Bioengineering, and Biodiversity, 2972 , 2023. doi: 10.1063/5.0184833

Last update: 2025-05-05 20:01:55

  1. Anti-inflammatory, pro-proliferative and antimicrobial potential of the compounds isolated from Daemonorops draco (Willd.) Blume

    L. Apaza Ticona, Á. Rumbero Sánchez, J. Sánchez Sánchez-Corral, P. Iglesias Moreno, M. Ortega Domenech. Journal of Ethnopharmacology, 268 , 2021. doi: 10.1016/j.jep.2020.113668