Cytotoxic Activity of Ethyl-para-methoxycinnamate from Kaempferia galanga L. on A549 Lung Cancer and B16 Melanoma Cancer Cells

Riza Apriani; Fajar Fauzi Abdullah

doi:10.14710/jksa.24.1.22-28

DOI: https://doi.org/10.14710/jksa.24.1.22-28

Cytotoxic Activity of Ethyl-para-methoxycinnamate from Kaempferia galanga L. on A549 Lung Cancer and B16 Melanoma Cancer Cells

Riza Apriani

, Fajar Fauzi Abdullah

Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Garut, Indonesia

Received: 6 Dec 2020; Revised: 27 Feb 2020; Accepted: 28 Feb 2020; Available online: 28 Feb 2020; Published: 28 Feb 2021.

BibTex Citation Data :

@article{JKSA34773,
    author = {Riza Apriani and Fajar Abdullah},
    title = {Cytotoxic Activity of Ethyl-para-methoxycinnamate from Kaempferia galanga L. on A549 Lung Cancer and B16 Melanoma Cancer Cells},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {24},
    number = {1},
    year = {2021},
    keywords = {A549 cell; B16 cell; cytotoxic; Ethyl-p-methoxycinnamate (EPMC), Kaempferia galanga L},
    abstract = {  Kaempferia galanga  L. belongs to the family of Zingiberaceae, an endangered medicinal plant with pharmacology activities. Ethyl- p -methoxycinnamate (EPMC) is an essential phytoconstituent of  K. galanga  rhizomes. Several studies have reported that EPMC has anticancer activities in several cancer cells, including CL-6 gallbladder cancer cells, HepG2liver cancer cells, MCF-7 breast cancer cells, and Raji lymphoma cancer cells. However, studies on A549 lung cancer and B16 melanoma cancer cells have not been reported. This study aimed to determine the anticancer activity of EPMC against A549 lung cancer and B16 melanoma cancer cells. EPMC was obtained by extraction using  n -hexane, then recrystallized with chloroform. The isolate was then analyzed by thin-layer chromatography (TLC), and the structure was characterized by Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Cytotoxic activity was determined under Presto Blue assay. Based on the result, EPMC from  K. galanga  showed the cytotoxic effect on B16 cells with an IC 50  value of 97.09 μg/mL, whereas EPMC showed no significant cytotoxic effect on A549 with an IC 50  value of 1407.75 μg/mL. It was concluded that EPMC has potential cytotoxic on B16 melanoma cancer cells, but it showed inactive activity against A549 lung cancer cells. Further molecular mechanism underlying EPMC cytotoxic activity needs to be conducted. },
   issn = {2597-9914},   pages = {22--28}  doi = {10.14710/jksa.24.1.22-28},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/34773}
}

Citation Format:

Abstract

Kaempferia galanga L. belongs to the family of Zingiberaceae, an endangered medicinal plant with pharmacology activities. Ethyl-p-methoxycinnamate (EPMC) is an essential phytoconstituent of K. galanga rhizomes. Several studies have reported that EPMC has anticancer activities in several cancer cells, including CL-6 gallbladder cancer cells, HepG2liver cancer cells, MCF-7 breast cancer cells, and Raji lymphoma cancer cells. However, studies on A549 lung cancer and B16 melanoma cancer cells have not been reported. This study aimed to determine the anticancer activity of EPMC against A549 lung cancer and B16 melanoma cancer cells. EPMC was obtained by extraction using n-hexane, then recrystallized with chloroform. The isolate was then analyzed by thin-layer chromatography (TLC), and the structure was characterized by Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Cytotoxic activity was determined under Presto Blue assay. Based on the result, EPMC from K. galanga showed the cytotoxic effect on B16 cells with an IC₅₀ value of 97.09 μg/mL, whereas EPMC showed no significant cytotoxic effect on A549 with an IC₅₀ value of 1407.75 μg/mL. It was concluded that EPMC has potential cytotoxic on B16 melanoma cancer cells, but it showed inactive activity against A549 lung cancer cells. Further molecular mechanism underlying EPMC cytotoxic activity needs to be conducted.

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Keywords: A549 cell; B16 cell; cytotoxic; Ethyl-p-methoxycinnamate (EPMC), Kaempferia galanga L

Funding: Kementerian Riset dan Teknologi/Badan Riset dan Inovasi Nasional (RISTEK-BRIN) under contract 044.5/LEMLIT/UNIGA/IV/2020; FMIPA Universitas Garut under contract 07/BAPPD_UPPM/XI/2019

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Section: Research Articles

Language : EN

In Vol 24, No 1 (2021): Volume 24 Issue 1 Year 2021

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