DOI: https://doi.org/10.14710/jksa.26.1.10-18

Antioxidant Activity of Flavonoids from Cassava Leaves (Manihot esculenta Crantz)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia

Received: 7 Jan 2022; Revised: 15 Dec 2022; Accepted: 23 Dec 2022; Published: 31 Jan 2023.
Open Access Copyright 2023 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

The community uses cassava leaves for cooking because of their high protein content, β-carotene, Fe, Mg, Zn, S, Ca, Zn, Ni, and K. Cassava leaves contain various secondary metabolites, including flavonoids, saponins, tannins, phenolics, steroids, anthocyanins, and anthraquinones. The antioxidant activity of flavonoid compounds in cassava leaves (Manihot esculenta Crantz) has been successfully investigated. This study consisted of six stages: the preparation of ethanol extract of cassava leaves, phytochemical screening, determination of total flavonoid content in the extract, isolation of flavonoids, and purity testing of flavonoid isolates using the chromatographic method, identification of the structure of pure flavonoid isolates using a UV-Vis spectrophotometer with the addition of a shear reagent, FTIR, and LC-MS/MS. As well as test the antioxidant activity using the DPPH method. The yield of ethanol extract from cassava leaf in this study was 14.67%. The results of the phytochemical screening showed that the cassava leaves and the ethanol extract of cassava leaves contained alkaloids, flavonoids, saponins, tannins, quinones, steroids, phenolics, and triterpenoids. The total content of flavonoids in the ethanol extract was 35.71 mg EQ/g extract. UV-Vis, FTIR, and LC-MS/MS analysis revealed that the flavonoid isolate was quercetin. The results of the antioxidant activity of the ethanol extract and BC isolate showed IC50 of 81.76 ± 0.505 mg/L and 77.85 ± 4.708 mg/L.

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Keywords: Manihot esculenta Crantz; flavonoids; total flavonoids; antioxidants
Funding: Universitas Diponegoro

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Section: Research Articles
Language : EN
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  1. Eka Handayanta, Optimalisasi Penggunaan Daun Ubikayu sebagai Pakan Ternak Ruminansia, CarakaTani: Journal of Sustainable Agriculture, 17, 2, (2018), 41-48 https://doi.org/10.20961/carakatani.v17i2.20122
  2. Hermanto Hermanto, Fitriani Fitriani, Pemanfaatan Limbah Kulit dan Daun Singkong sebagai Campuran Bahan Pakan Ternak Unggas, Jurnal Riset Teknologi Industri, 13, 2, (2019), 284-295 http://dx.doi.org/10.26578/jrti.v13i2.5610
  3. Delphin Diasolua Ngudi, Yu‐Haey Kuo, Fernand Lambein, Amino acid profiles and protein quality of cooked cassava leaves or ‘saka‐saka’, Journal of the Science of Food and Agriculture, 83, 6, (2003), 529-534 https://doi.org/10.1002/jsfa.1373
  4. Carmen Wobeto, Angelita Duarte Corrêa, Celeste Maria Pato de Abreu, Custódio Donizete dos Santos, José Renato de Abreu, Nutrients in the cassava (Manihot esculenta Crantz) leaf meal at three ages of the plant, Food Science and Technology, 26, 4, (2006), 865-869 https://doi.org/10.1590/S0101-20612006000400024
  5. Ayodeji O. Fasuyi, Nutrient composition and processing effects on cassava leaf (Manihot esculenta, Crantz) antinutrients, Pakistan Journal of Nutrition, 4, 1, (2005), 37-42 https://dx.doi.org/10.3923/pjn.2005.37.42
  6. S. Anbuselvi, T. Balamurugan, Phytochemical and antinutrient constituents of cassava and sweet potato, World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS), 3, 3, (2014), 1440-1449
  7. Faezah O. Nur, Aishah H. Siti, Kalsom Y. Umi, Comparative evaluation of organic and inorganic fertilizers on total phenolic, total flavonoid, antioxidant activity and cyanogenic glycosides in cassava (Manihot esculenta), African Journal of Biotechnology, 12, 18, (2013), 2414-2421
  8. Haiteng Tao, Bo Cui, Hongxia Zhang, Alaa El-Din Bekhit, Feijie Lu, Identification and characterization of flavonoids compounds in cassava leaves (Manihot esculenta Crantz) by HPLC/FTICR-MS, International Journal of Food Properties, 22, 1, (2019), 1134-1145 https://doi.org/10.1080/10942912.2019.1626879
  9. Setya Enti Rikomah, Elmitra Elmitra, Diana Gustina Yunita, Efek ekstrak etanol daun singkong (Manihot utilissima pohl) sebagai obat alternatif anti rematik terhadap rasa sakit pada mencit, Jurnal Ilmiah Manuntung, 3, 2, (2017), 133-138
  10. Resmi Mustarichie, Sulistiyaningsih Sulistyaningsih, Dudi Runadi, Antibacterial activity test of extracts and fractions of cassava leaves (Manihot esculenta Crantz) against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes causing acne, International Journal of Microbiology, 2020, (2020), 1975904 https://doi.org/10.1155/2020/1975904
  11. Auronita Puspa Pratiwi, Aktivitas Antibakteri Ekstrak Daun Singkong (Manihot esculenta Crantz.) terhadap Shigella sp, Jurnal Kesehatan, 7, 1, (2016), 161-164 http://dx.doi.org/10.26630/jk.v7i1.134
  12. Azmi Prasasti, Anung Kustriyani, Velisia Dini Permatasari, Putri Oktalia, Nursiyatin Nursiyatin, Uji Aktivitas Antibakteri Daun Singkong (Manihot esculenta) Pada Bakteri Staphylococcus aureus dan Eschrichia coli, Bioeksperimen: Jurnal Penelitian Biologi, 8, 1, (2022), 57-64 https://doi.org/10.23917/bioeksperimen.v8i1.16133
  13. Isnatin Miladiyah, Analgesic activity of ethanolic extract of Manihot esculenta Crantz leaves in mice, Universa Medicina, 30, 1, (2011), 3-10
  14. Satish E. Bahekar, Ranjana S. Kale, Antidiarrheal activity of ethanolic extract of Manihot esculenta Crantz leaves in Wistar rats, Journal of Ayurveda and Integrative Medicine, 6, 1, (2015), 35-40
  15. N. K. Warditiani, L. P. F. Larasanty, I. Damanik, Pengaruh pemberian ekstrak etanol 70% daun singkong (Manihot utilissima pohl) terhadap kadar gula darah mencit jantan galur balb/c yang diinduksi aloksan, Jurnal Farmasi Udayana, 4, 1, (2015), 61-64
  16. F. Ekawasti, Suhardono Suhardono, D. A. Dewi, E. Martindah, A. H. Wardhana, D. H. Sawitri, Skrining Efektivitas Ekstrak Tanaman Herbal sebagai Anthelmintik terhadap Telur dan Larva Nematoda serta Cacing Haemonchus contortus secara In-Vitro, Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner, 2020
  17. Ebenezer Idowu Ajayi, Amit Agarwal, Uttam Chand Banerjee, Olufunso Olabode Olorunsogo, Ethanol extract of Manihot esculenta leaf: A potential source of antioxidant, xanthine oxidase and lipase inhibitors, Analele Stiintifice Ale Univ Alexandru Ioan Cuza Din Iasi Sec II Genet Si Biol Mol, 18, 1, (2017), 17 23
  18. Restanti Solikhah, Eling Purwantoyo, Ely Rudyatmi, Aktivitas antioksidan dan kadar klorofil kultivar singkong di daerah Wonosobo, Life Science, 8, 1, (2019), 86-95
  19. La Jumadin, Aryani Sismin Satyaningtijas, Koekoeh Santoso, Ekstrak daun singkong baik sebagai antioksidan pada burung puyuh dewasa yang mendapat paparan panas singkat, Jurnal Veteriner, 18, 1, (2017), 135-143 https://doi.org/10.19087/jveteriner.2017.18.1.135
  20. E. Quartey, H. Amoatey, E. Achoribo, M. Owusu-Ansah, W. Nunekpeku, S. Donkor, A. S. Appiah, E. S. K. Ofori, Phytochemical constituents and antioxidant activities in leaves of 14 breeding lines of cassava (Manihot esculenta Crantz), American Journal of Experimental Agriculture, 12, 5, (2016), 1-10 https://doi.org/10.9734/AJEA/2016/18087
  21. Putri Indah Sayakti, Norma Anisa, Hafiz Ramadhan, Antioxidant activity of methanol extract of cassava leaves (Manihot esculenta Crantz) using CUPRAC method, Jurnal Ilmiah Farmasi, Special Edition, (2022), 97-106 https://doi.org/10.20885/jif.specialissue2022.art12
  22. Hasim Hasim, Syamsul Falah, Lia Kusuma Dewi, Effect of boiled cassava leaves (Manihot esculenta Crantz) on total phenolic, flavonoid and its antioxidant activity, Current Biochemistry, 3, 3, (2016), 116-127
  23. Fauzy Rachman, Sri Hartati, Enny Sudarmonowati, Partomuan Simanjuntak, Aktivitas Antioksidan Daun Dan Umbi Dari Enam Jenis Singkong (Manihot utilissima Pohl), Biopropal Industri, 7, 2, (2016), 47 52
  24. Enny Fachriyah, Dewi Kusrini, Ifan Bagus Haryanto, Synta Mutiara Bunga Wulandari, Widyaningrum Islami Lestari, Sumariyah Sumariyah, Phytochemical test, determination of total phenol, total flavonoids and antioxidant activity of ethanol extract of Moringa leaves (Moringa oleifera Lam), Jurnal Kimia Sains dan Aplikasi, 23, 8, (2020), 290-294 https://doi.org/10.14710/jksa.23.8.290-294
  25. Norman R. Farnsworth, Biological and phytochemical screening of plants, Journal of Pharmaceutical Sciences, 55, 3, (1966), 225-276 https://doi.org/10.1002/jps.2600550302
  26. Chia-Chi Chang, Ming-Hua Yang, Hwei-Mei Wen, Jiing-Chuan Chern, Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis, 10, 3, (2002), https://doi.org/10.38212/2224-6614.2748
  27. Tom J. Mabry, Kenneth R. Markham, Michael Barrie Thomas, The systematic identification of flavonoids, 1 ed., Springer Berlin, Heidelberg, 2012, ^ https://doi.org/10.1007/978-3-642-88458-0
  28. Jeffrey B. Harborne, Metode fitokimia: Penuntun cara modern menganalisis tumbuhan, K. Padmawinata, I. Soediro, Penerbit ITB, Bandung, 1987
  29. Philip Molyneux, The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin Journal of Science and Technology, 26, 2, (2004), 211-219
  30. Vajjiram Chinnadurai, Periannan Viswanathan, Kandasamy Kalimuthu, Ammasai Vanitha, Venkatachalam Ranjitha, Arivalagan Pugazhendhi, Comparative studies of phytochemical analysis and pharmacological activities of wild and micropropagated plant ethanol extracts of Manihot esculenta, Biocatalysis and Agricultural Biotechnology, 19, (2019), 101166 https://doi.org/10.1016/j.bcab.2019.101166
  31. Mira Jun, H.‐Y. Fu, J. Hong, Xia Wan, C. S. Yang, C.‐T. Ho, Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria lobata Ohwi), Journal of Food Science, 68, 6, (2003), 2117-2122 https://doi.org/10.1111/j.1365-2621.2003.tb07029.x
  32. Yi-Zhong Cai, Mei Sun, Jie Xing, Qiong Luo, Harold Corke, Structure–radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants, Life Sciences, 78, 25, (2006), 2872-2888 https://doi.org/10.1016/j.lfs.2005.11.004
  33. Hong-Yu Lin, Yueh-Hsiung Kuo, Yun-Lian Lin, Wenchang Chiang, Antioxidative effect and active components from leaves of Lotus (Nelumbo nucifera), Journal of Agricultural and Food Chemistry, 57, 15, (2009), 6623-6629 https://doi.org/10.1021/jf900950z

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