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Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia

Received: 9 Oct 2022; Revised: 25 Nov 2022; Accepted: 20 Dec 2022; Published: 31 Dec 2022.
Open Access Copyright 2022 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
Oxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content of 2% to 30%. These bacteria can produce various kinds of pigments for self-defense from extreme environments, which function as immunomodulatory compounds and have antioxidant activity. The antioxidant activity of the yellow pigment halophilic bacteria Bacillus clausii J1G-0%B using the DPPH method showed that the effect of light and air on antioxidant activity was 17.88% inhibition in the crude extract and 14.24% inhibition at 1600 ppm. Antioxidant activity decreased by 17.13% under the influence of air, 1.89% under the influence of light, and 28.58% under the influence of air and light. FTIR analysis under the influence of air experienced a decrease in the peak of the CO carbonyl group of 1739.29 cm-1 and an increase in the alkane C-H group of 1369.52 cm-1, while under the influence of light and open conditions, the carbonyl CO group experienced an increase of 1739.50 cm-1 and 1739.46 cm-1 and the increase in alkane C-H groups 1371.41 cm-1 and 1369.53 cm- 1.
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Keywords: halophilic; Bacillus clausii; antioxidant; pigment
Funding: Universitas Diponegoro

Article Metrics:

  1. Vijaya Lobo, Avinash Patil, A. Phatak, Naresh Chandra, Free radicals, antioxidants and functional foods: Impact on human health, Pharmacognosy Reviews, 4, 8, (2010), 118-126 http://dx.doi.org/10.4103/0973-7847.70902
  2. Atasoy Nurhayat, Yücel Ufuk Mercan, Antioxidants from Plant Sources and Free Radicals, in: A. Rizwan (Ed.) Reactive Oxygen Species, IntechOpen, Rijeka, 2021, https://doi.org/10.5772/intechopen.100350
  3. Mehdi Sharifi-Rad, Nanjangud V. Anil Kumar, Paolo Zucca, Elena Maria Varoni, Luciana Dini, Elisa Panzarini, Jovana Rajkovic, Patrick Valere Tsouh Fokou, Elena Azzini, Ilaria Peluso, Abhay Prakash Mishra, Manisha Nigam, Youssef El Rayess, Marc El Beyrouthy, Letizia Polito, Marcello Iriti, Natália Martins, Miquel Martorell, Anca Oana Docea, William N. Setzer, Daniela Calina, William C. Cho, Javad Sharifi-Rad, Lifestyle, oxidative stress, and antioxidants: back and forth in the pathophysiology of chronic diseases, Frontiers in Physiology, 11, 694, (2020)
  4. Joanna Fiedor, Květoslava Burda, Potential Role of Carotenoids as Antioxidants in Human Health and Disease, Nutrients, 6, 2, (2014), 466-488 https://doi.org/10.3390/nu6020466
  5. T. P. A. Devasagayam, J. C. Tilak, K. K. Boloor, Ketaki S. Sane, Saroj S. Ghaskadbi, R. D. Lele, Free radicals and antioxidants in human health: current status and future prospects, Journal of the Association of Physicians of India, 52, (2004), 794-804
  6. Jeganathan Arun, Sundararaj Selvakumar, Ramamoorthy Sathishkumar, Meivelu Moovendhan, Gnanakkan Ananthan, Thirumalai Maruthiah, Arunachalam Palavesam, In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis, Carbohydrate Polymers, 155, (2017), 400-406 https://doi.org/10.1016/j.carbpol.2016.08.085
  7. Jolanta Flieger, Wojciech Flieger, Jacek Baj, Ryszard Maciejewski, Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles, Materials, 14, 15, (2021), 4135 https://doi.org/10.3390/ma14154135
  8. Fereidoon Shahidi, Priyatharini Ambigaipalan, Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review, Journal of Functional Foods, 18, (2015), 820-897 https://doi.org/10.1016/j.jff.2015.06.018
  9. Rie Yatsunami, Ai Ando, Ying Yang, Shinichi Takaichi, Masahiro Kohno, Yuriko Matsumura, Hiroshi Ikeda, Toshiaki Fukui, Kaoru Nakasone, Nobuyuki Fujita, Mitsuo Sekine, Tomonori Takashina, Satoshi Nakamura, Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica, Frontiers in Microbiology, 5, (2014), https://doi.org/10.3389/fmicb.2014.00100
  10. Priya DasSarma, James A. Coker, Valerie Huse, Shiladitya DasSarma, in: M.C. Flickinger (Ed.) Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, John Wiley & Sons, Inc, 2009, p. 1-9
  11. A. Ventosa, J. J. Nieto, Biotechnological applications and potentialities of halophilic microorganisms, World Journal of Microbiology & Biotechnology, 11, (1995), 85-94 https://doi.org/10.1007/BF00339138
  12. John T. Landrum, Chemical Breakdown of Carotenoids In Vitro and In Vivo, in: J.T. Landrum (Ed.) Carotenoids: Physical, Chemical, and Biological Functions and Properties, CRC Press, Boca Raton, 2009
  13. Sari Pratiwi, Identifikasi Genotipik dan Aktivitas Antioksidan Pigmen Kuning Mikroorganisme Halofilik J1G-0% dari Tambak Garam Madura, Universitas Diponegoro, Semarang, 2019
  14. Ita Redyaningsih, Identifikasi Mikroorganisme Halofilik J1G-0% dari Tambak Garam Madura dan Uji Antibakteri Pigmen Oranye dan Metabolit Sekundernya, Universitas Diponegoro, Semarang
  15. Mario C. Foti, Riccardo Amorati, Non-phenolic radical-trapping antioxidants, Journal of Pharmacy and Pharmacology, 61, 11, (2009), 1435-1448 https://doi.org/10.1211/jpp.61.11.0002
  16. Chonglong Wang, Shuli Zhao, Xixi Shao, Ji-Bin Park, Seong-Hee Jeong, Hyo-Jin Park, Won-Ju Kwak, Gongyuan Wei, Seon-Won Kim, Challenges and tackles in metabolic engineering for microbial production of carotenoids, Microbial Cell Factories, 18, (2019), 55 https://doi.org/10.1186/s12934-019-1105-1
  17. Przemysław Adamkiewicz, Agnieszka Sujak, Wiesław I. Gruszecki, Spectroscopic study on formation of aggregated structures by carotenoids: Role of water, Journal of Molecular Structure, 1046, (2013), 44-51 https://doi.org/10.1016/j.molstruc.2013.04.053
  18. Thais De Nardo, Cecilia Shiroma-Kian, Yuwana Halim, David Francis, Luis E. Rodriguez-Saona, Rapid and Simultaneous Determination of Lycopene and β-Carotene Contents in Tomato Juice by Infrared Spectroscopy, Journal of Agricultural and Food Chemistry, 57, 4, (2009), 1105-1112 https://doi.org/10.1021/jf802920z
  19. M. Baranska, W. Schütze, H. Schulz, Determination of Lycopene and β-Carotene Content in Tomato Fruits and Related Products: Comparison of FT-Raman, ATR-IR, and NIR Spectroscopy, Analytical Chemistry, 78, 24, (2006), 8456-8461 https://doi.org/10.1021/ac061220j
  20. S. Ghodbane, A. Deneuville, D. Tromson, P. Bergonzo, E. Bustarret, D. Ballutaud, Sensitivity of Raman spectra excited at 325 nm to surface treatments of undoped polycrystalline diamond films, physica status solidi (a), 203, 10, (2006), 2397-2402 https://doi.org/10.1002/pssa.200521462
  21. Chinnathambi Pothiraj, Paulraj Balaji, Ramkumar Shanthi, Muthukrishnan Gobinath, Rangasamy Suresh Babu, Abdullah Al-Dosary Munirah, Atef Hatamleh Ashraf, Kamatchi Ramesh Kumar, Veeramani Veeramanikandan, Ramasubramanian Arumugam, Evaluating antimicrobial activities of Acanthus ilicifolius L. and Heliotropium curassavicum L against bacterial pathogens: an in-vitro study, Journal of Infection and Public Health, 14, 12, (2021), 1927-1934 https://doi.org/10.1016/j.jiph.2021.10.013
  22. Yang Gyu Ku, Ho Cheol Kim, Jong Hyang Bae, Baek Song Kang, Alina Nemirovski, Dinorah Barasch, Shela Gorinstein, Antioxidant capacities and polyphenols in autumn-growing cultivar of Chinese cabbage (Brassica rapa L. ssp. pekinensis cv. Bulam Plus), European Food Research and Technology, 245, 9, (2019), 1871-1879 https://doi.org/10.1007/s00217-019-03294-0

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