Department of Pharmacy, Faculty of Pharmacy, Bakti Tunas Husada University, Tasikmalaya, Indonesia
BibTex Citation Data :
@article{JKSA68508, author = {Lilis Tuslinah and Laras Widyastuti and Mochamad Fathurohman}, title = {Validation of the Use of Anthocyanin as Indicators for Acid-Base Titration}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {28}, number = {2}, year = {2025}, keywords = {Rambutan Peel (Nephelium lappaceum L.); Anthocyanins; Acid-Base Titration Indicator; Method Validation}, abstract = { Rambutan peel ( Nephelium lappaceum L. ) contains secondary metabolites, particularly flavonoids, including anthocyanins. Anthocyanins exhibit color changes in response to variations in pH, making them potential candidates for use as acid-base indicators. This study aims to validate using methanol fraction powder derived from rambutan peel as an acid-base titration indicator by evaluating method validation parameters such as precision, accuracy, and linearity. The fractionation process was conducted using liquid-liquid extraction with n -hexane, water, and methanol as solvents, followed by freeze-drying of the methanol fraction. The methanol fraction powder was analyzed and compared with the conventional phenolphthalein indicator to determine the color transition at the titration endpoint. The results indicated that the methanol fraction exhibited a color change to brown within a pH range of 8–11. Statistical analysis using an independent sample t -test showed no significant difference between the methanol fraction powder and phenolphthalein (significance value > 0.05). Therefore, based on the method validation results, including precision, accuracy, and linearity, the methanol fraction powder of rambutan peel demonstrates potential as a reliable acid-base indicator. }, issn = {2597-9914}, pages = {53--61} doi = {10.14710/jksa.28.2.53-61}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/68508} }
Refworks Citation Data :
Rambutan peel (Nephelium lappaceum L.) contains secondary metabolites, particularly flavonoids, including anthocyanins. Anthocyanins exhibit color changes in response to variations in pH, making them potential candidates for use as acid-base indicators. This study aims to validate using methanol fraction powder derived from rambutan peel as an acid-base titration indicator by evaluating method validation parameters such as precision, accuracy, and linearity. The fractionation process was conducted using liquid-liquid extraction with n-hexane, water, and methanol as solvents, followed by freeze-drying of the methanol fraction. The methanol fraction powder was analyzed and compared with the conventional phenolphthalein indicator to determine the color transition at the titration endpoint. The results indicated that the methanol fraction exhibited a color change to brown within a pH range of 8–11. Statistical analysis using an independent sample t-test showed no significant difference between the methanol fraction powder and phenolphthalein (significance value > 0.05). Therefore, based on the method validation results, including precision, accuracy, and linearity, the methanol fraction powder of rambutan peel demonstrates potential as a reliable acid-base indicator.
Article Metrics:
Last update:
Last update: 2026-10-15 13:11:38
As an article writer, the author has the right to use their article for various purposes, including use by institutions that employ the author or institutions that provide funding for the research. These author rights are granted without special permission.
Authors who publish a paper in JKSA have broad rights to use their work for teaching and scientific purposes without the need to request permission, including: (i) teaching in the author's class or institution; (ii) presenting the work at meetings or conferences and distributing copies to participants; (iii) using the work in training conducted by the author or the author's institution; (iv) distributing copies to colleagues for research purposes; (v) using the work in the compilation of subsequent works by the author; (vi) including the work in a thesis or dissertation; (vii) reusing part of the article in another work, with appropriate citation; (viii) preparing derivative works, with appropriate citation; and (ix) voluntarily posting the work on open websites operated by the author or the author's institution for scientific purposes, in accordance with the CC BY-SA License.
Authors and readers may copy and redistribute the material in any medium or format, and may remix, transform, and build upon the material for any purpose, provided that they give appropriate credit by citing the article or its content, provide a link to the license, and indicate if changes were made.
The authors submitting a manuscript do so on the understanding that, if accepted for publication, the copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses the rights to reproduce and distribute the article in all forms and media, including reprints, photographs, microfilms, and any other similar reproductions, as well as translations.
Reproducing any part of this journal, storing it in a database, or transmitting it in any form or medium is permitted without written permission from JKSA. However, the original source must be properly cited in accordance with academic conventions.
JKSA, the Chemistry Department of Diponegoro University, and the Editor make every effort to ensure that no inaccurate, false, or misleading data, opinions, or statements are published in JKSA. However, the content of each article is the sole and exclusive responsibility of its authors.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should bear the authors' original signatures and be sent to the Editor as a printed document, a scanned document sent via email, or by fax.
Prof. Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
