DOI: https://doi.org/10.14710/jksa.28.3.146-154

Validation Method of the Cellulose Triacetate-Based Optode Membrane for Fe(III) Detection in Water Samples

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia

2Tropical Biopharmaca Research Center, IPB University, Bogor, Indonesia

Received: 28 Dec 2024; Revised: 8 Apr 2025; Accepted: 8 Apr 2025; Published: 30 Apr 2025.
Open Access Copyright 2025 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

Iron contamination in water is a threat to human health and the environment. This contamination requires a fast and efficient detection method. Method validation is integral to method development, ensuring alignment with the intended objectives. This research aims to validate a method for selective detection of Fe(III) based on cellulose triacetate (CTA) optode membrane. The optode membrane was prepared by mixing CTA, plasticizers, Aliquat-336, and thiocyanate as selective reagents. The validation of the optode membrane was evaluated based on validation performance parameters. The Fe(III) optode membrane detection demonstrated a linear response with a determination coefficient of 0.9972 within a concentration range of 0.1–4.0 mg/L, the detection limit of 0.0553 mg/L, quantitation limit of 0.1676 mg/L, precision 3.01%, intermediate precision of 3.03% and 3.01%, the accuracy of 101.62%. The optode membrane exhibited good selectivity with a value of -0.4580 and -0.2748 against Pb(II) and Cr(VI), respectively, sensitivity of 1.05 × 107 M-1 cm-1 and color formation stability %RSD of 3.14%. The application of real samples shows no significant difference between the UV-Vis spectrophotometry and optode membrane methods at a 95% confidence level (α = 0.05). The validation results offer a valuable perspective into whether this method can be adopted as a new approach or as an alternative to existing methods for cation analysis in water samples.

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Keywords: Cellulose triacetate; Fe(III); Optode Membrane; Method Validation
Funding: Directorate General of Higher Education, Research, and Technology, Ministry under contract 027/E5/PG.02.00.PL/2024 of Education, Culture, Research, and Technology of the Republic of Indonesia under contract 027/E5/PG.02.00.PL/2024

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Section: Research Articles
Language : EN
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