skip to main content

Chitosan/Anthocyanin-Based Indicator Film with Carnauba Wax Addition for Monitoring Chicken Freshness

Department of Chemistry, State University of Semarang, Semarang 50221, Indonesia

Received: 24 Jan 2025; Revised: 21 Jun 2025; Accepted: 24 Jun 2025; Published: 5 Aug 2025.
Open Access Copyright 2025 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract
Indicator films can be applied to food packaging to monitor the quality of food products. However, chitosan/anthocyanin films tend to have low hydrophobicity, which may compromise their performance as indicators and lead to dye migration. One approach to address this issue is by incorporating additional materials such as carnauba wax. This study investigates the effect of carnauba wax addition on the mechanical properties and water contact angle of chitosan/anthocyanin-based indicator films, as well as evaluates the performance of the resulting films in monitoring the freshness of chicken meat. Indicator films were prepared by adding carnauba wax at concentrations of 6, 9, 12, and 15% (w/w of chitosan). The results showed that the addition of carnauba wax improved the mechanical properties of the films, although the improvement was not statistically significant. The best mechanical performance was observed with the 15% wax formulation. Additionally, the water contact angle increased with higher concentrations of carnauba wax, with the highest value (71.630°) observed for the CS/ABT/CW15% film. The indicator films were applied to chicken packaging and evaluated based on color changes during storage as a measure of freshness. After 30 hours, the film color changed from dark brown to green. The color change pattern during storage was similar for films with and without carnauba wax, indicating that the addition of carnauba wax does not affect the film’s ability to monitor chicken freshness.
Fulltext View|Download
Keywords: indicator film; chitosan; anthocyanin; carnauba wax; chicken meat

Article Metrics:

  1. Reskiati Wiradhika Anwar, Analisis dan Desain Sistem Produksi Kemasan Aktif Antimikroba Untuk Menjaga Mutu Produk Pangan, Agrointek: Jurnal Teknologi Industri Pertanian, 17, 2, (2023), 394-403
  2. Milad Ranjbar, Mohammad Hossein Azizi Tabrizzad, Gholamhassan Asadi, Hamed Ahari, Investigating the microbial properties of sodium alginate/chitosan edible film containing red beetroot anthocyanin extract for smart packaging in chicken fillet as a pH indicator, Heliyon, 9, 8, (2023), e18879 https://doi.org/10.1016/j.heliyon.2023.e18879
  3. Luman Zheng, Liming Liu, Jiahao Yu, Ping Shao, Novel trends and applications of natural pH-responsive indicator film in food packaging for improved quality monitoring, Food Control, 134, (2022), 108769 https://doi.org/10.1016/j.foodcont.2021.108769
  4. Mahmood Alizadeh-Sani, Milad Tavassoli, Esmail Mohammadian, Ali Ehsani, Gholamreza Jahed Khaniki, Ruchir Priyadarshi, Jong-Whan Rhim, pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness, International Journal of Biological Macromolecules, 166, (2021), 741-750 https://doi.org/10.1016/j.ijbiomac.2020.10.231
  5. Simin Pourjavaher, Hadi Almasi, Saeed Meshkini, Sajad Pirsa, Ehsan Parandi, Development of a colorimetric pH indicator based on bacterial cellulose nanofibers and red cabbage (Brassica oleraceae) extract, Carbohydrate Polymers, 156, (2017), 193-201 https://doi.org/10.1016/j.carbpol.2016.09.027
  6. Parya Ezati, Jong-Whan Rhim, pH-responsive chitosan-based film incorporated with alizarin for intelligent packaging applications, Food Hydrocolloids, 102, (2020), 105629 https://doi.org/10.1016/j.foodhyd.2019.105629
  7. Kyu Jin Park, Ji-Soo Lee, Hae Jee Jo, Eun Suh Kim, Hyeon Gyu Lee, Antimicrobial and indicator properties of edible film containing clove bud oil-loaded chitosan capsules and red cabbage for fish preservation, International Journal of Biological Macromolecules, 196, (2022), 163-171 https://doi.org/10.1016/j.ijbiomac.2021.12.027
  8. Meiyu Chen, Tianyi Yan, Jiayin Huang, Yaqi Zhou, Yaqin Hu, Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring, International Journal of Biological Macromolecules, 179, (2021), 90-100 https://doi.org/10.1016/j.ijbiomac.2021.02.170
  9. Fahimeh Ebrahimi Tirtashi, Mehran Moradi, Hossein Tajik, Mehrdad Forough, Parya Ezati, Bambang Kuswandi, Cellulose/chitosan pH-responsive indicator incorporated with carrot anthocyanins for intelligent food packaging, International Journal of Biological Macromolecules, 136, (2019), 920-926 https://doi.org/10.1016/j.ijbiomac.2019.06.148
  10. Ayman Nafady, Abdullah M. Al-Enizi, Asma A. Alothman, Shoyebmohamad F. Shaikh, Design and fabrication of green and sustainable vapochromic cellulose fibers embedded with natural anthocyanin for detection of toxic ammonia, Talanta, 230, (2021), 122292 https://doi.org/10.1016/j.talanta.2021.122292
  11. Sudarshan Singh, Ozioma Forstinus Nwabor, Dwi Marlina Syukri, Supayang Piyawan Voravuthikunchai, Chitosan-poly(vinyl alcohol) intelligent films fortified with anthocyanins isolated from Clitoria ternatea and Carissa carandas for monitoring beverage freshness, International Journal of Biological Macromolecules, 182, (2021), 1015-1025 https://doi.org/10.1016/j.ijbiomac.2021.04.027
  12. Kailong Zhang, Tung-Shi Huang, Hao Yan, Xinzhong Hu, Tian Ren, Novel pH-sensitive films based on starch/polyvinyl alcohol and food anthocyanins as a visual indicator of shrimp deterioration, International Journal of Biological Macromolecules, 145, (2020), 768-776 https://doi.org/10.1016/j.ijbiomac.2019.12.159
  13. Qiuyi Li, Pingxiong Cai, Huining Xiao, Yuanfeng Pan, pH-responsive color indicator films based on chitosan and purple yam extract for in-situ monitoring food freshness, Food Bioscience, 56, (2023), 103373 https://doi.org/10.1016/j.fbio.2023.103373
  14. Francisco Klebson Gomes dos Santos, Karyn Nathallye de Oliveira Silva, Talisson Davi Noberto Xavier, Ricardo Henrique de Lima Leite, Edna Maria Mendes Aroucha, Effect of the Addition of Carnauba Wax on Physicochemical Properties of Chitosan Films, Materials Research, 20, (2017), 479-484 https://doi.org/10.1590/1980-5373-MR-2016-1010
  15. Delina Muscat, Raju Adhikari, Stafford McKnight, Qipeng Guo, Benu Adhikari, The physicochemical characteristics and hydrophobicity of high amylose starch–glycerol films in the presence of three natural waxes, Journal of Food Engineering, 119, 2, (2013), 205-219 https://doi.org/10.1016/j.jfoodeng.2013.05.033
  16. Sulafa B. H. Hashim, Haroon Elrasheid Tahir, Li Lui, Junjun Zhang, Xiaodong Zhai, Amer Ali Mahdi, Nosyba A. Ibrahim, Gustav Komla Mahunu, Mahmoud M. Hassan, Zou Xiaobo, Shi Jiyong, Smart films of carbohydrate-based/sunflower wax/purple Chinese cabbage anthocyanins: A biomarker of chicken freshness, Food Chemistry, 399, (2023), 133824 https://doi.org/10.1016/j.foodchem.2022.133824
  17. Fransisca Widhi Mahatmanti, Mohammad Alauhdin, Serafica Btari Christiyani Kusumaningrum, Smart and Green Packaging Made from Chitosan-based Biofilm with the Addition of Ginger Oil and Anthocyanins from Butterfly Pea Flower Extract (Clitoria Ternatea L), Jurnal Kimia Sains dan Aplikasi, 27, 2, (2024), 53-63 https://doi.org/10.14710/jksa.27.2.53-63
  18. Lia Handayani, Sri Aprilia, Nasrul Arahman, Muhammad Roil Bilad, Identification of the anthocyanin profile from butterfly pea (Clitoria ternatea L.) flowers under varying extraction conditions: Evaluating its potential as a natural blue food colorant and its application as a colorimetric indicator, South African Journal of Chemical Engineering, 49, (2024), 151-161 https://doi.org/10.1016/j.sajce.2024.04.008
  19. Mahmood Alizadeh-Sani, Esmail Mohammadian, Jong-Whan Rhim, Seid Mahdi Jafari, pH-sensitive (halochromic) smart packaging films based on natural food colorants for the monitoring of food quality and safety, Trends in Food Science & Technology, 105, (2020), 93-144 https://doi.org/10.1016/j.tifs.2020.08.014
  20. Josemar Gonçalves de Oliveira Filho, Anna Rafaela Cavalcante Braga, Bianca Ribeiro de Oliveira, Francileni Pompeu Gomes, Virgínia Lopes Moreira, Vinícius Alex Cano Pereira, Mariana Buranelo Egea, The potential of anthocyanins in smart, active, and bioactive eco-friendly polymer-based films: A review, Food Research International, 142, (2021), 110202 https://doi.org/10.1016/j.foodres.2021.110202
  21. Bao Chau K. Ly, Ethan B. Dyer, Jessica L. Feig, Anna L. Chien, Sandra Del Bino, Research Techniques Made Simple: Cutaneous Colorimetry: A Reliable Technique for Objective Skin Color Measurement, Journal of Investigative Dermatology, 140, 1, (2020), 3-12.e11 https://doi.org/10.1016/j.jid.2019.11.003
  22. Sulafa B. H. Hashim, Haroon Elrasheid Tahir, Li Liu, Junjun Zhang, Xiaodong Zhai, Amer Ali Mahdi, Faisal Nureldin Awad, Mahmoud M. Hassan, Zou Xiaobo, Shi Jiyong, Intelligent colorimetric pH sensoring packaging films based on sugarcane wax/agar integrated with butterfly pea flower extract for optical tracking of shrimp freshness, Food Chemistry, 373, (2022), 131514 https://doi.org/10.1016/j.foodchem.2021.131514
  23. Yaowen Liu, Yanlan Ma, Tan Feng, Jie Luo, Dur E. Sameen, Md Alomgir Hossen, Jianwu Dai, Suqing Li, Wen Qin, Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity, International Journal of Biological Macromolecules, 187, (2021), 566-574 https://doi.org/10.1016/j.ijbiomac.2021.07.132
  24. Sahid Nurul Syahida, Mohammad Rashedi Ismail-Fitry, Zuriyati Mohamed Asa’ari Ainun, Zainal Abedin Nur Hanani, Effects of palm wax on the physical, mechanical and water barrier properties of fish gelatin films for food packaging application, Food Packaging and Shelf Life, 23, (2020), 100437 https://doi.org/10.1016/j.fpsl.2019.100437
  25. Barbara Merz, Cristiane Capello, Gabriel Coelho Leandro, Denise Esteves Moritz, Alcilene Rodrigues Monteiro, Germán Ayala Valencia, A novel colorimetric indicator film based on chitosan, polyvinyl alcohol and anthocyanins from jambolan (Syzygium cumini) fruit for monitoring shrimp freshness, International Journal of Biological Macromolecules, 153, (2020), 625-632 https://doi.org/10.1016/j.ijbiomac.2020.03.048
  26. M. Yosi Prasetyo, Muhammad Hendri, Shaum Shiyan, Analisis Thermogravimetri dan Sifat Mekanis Edible Film Rumput Laut Gracillaria sp. Sebagai Bahan Alternatif Bioplastik, Jurnal Penelitian Sains, 26, 2, (2024), 147-155 https://doi.org/10.56064/jps.v26i2.1016
  27. Ratna Ratna, Selly Silvia Indriani, Hendri Syah, Pengaruh Konsentrasi Gliserol Terhadap Karakteristik Edible Film Gelatin, Jurnal Ilmiah Mahasiswa Pertanian, 9, 3, (2024), 237-251 https://doi.org/10.17969/jimfp.v9i3.29662
  28. Muhammad Fakih Kurniawan, Tiana Fitrilia, Hikmah Nur Chosida, Nursyawal Nacing, Sappan (Caesalpinia Sappan L.) Extract-Based Label for Detection of Chicken Meat Spoilage, Indonesian Journal of Applied Research (IJAR), 2, 3, (2021), 153-159 https://doi.org/10.30997/ijar.v2i3.146
  29. Dinda Denada, Arif Murtaqi Akhmad Mutsyahidan, Indriaty Lahamutu, Halimanti Manggena, Rifina Murtialmira, Studi Kasus Kualitas Daging Ayam Broiler di Pasar Sentral Kota Gorontalo, Jambura Journal of Tropical Livestock Science, 1, 2, (2023), 44-49

Last update:

No citation recorded.

Last update: 2025-08-19 05:47:27

No citation recorded.