The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines

Dessy Arisanty; Zolkapli Eshak; Fatma Sri Wahyuni; Afifatul Achyar; Salsabila Putri Khairani

doi:10.14710/jksa.29.1.%p

DOI: https://doi.org/10.14710/jksa.29.1.%25p

The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines

Dessy Arisanty^{1, 2}

, Zolkapli Eshak³

, Fatma Sri Wahyuni⁴

, Afifatul Achyar⁵

, Salsabila Putri Khairani⁶

¹Department of Biochemistry, Faculty of Medicine, Universitas Andalas, 25163, Padang, Indonesia

²Biomedical Science Study Program, Faculty of Medicine, Universitas Andalas, 25163, Padang, Indonesia

³Faculty of Pharmacy, Universiti Teknologi MARA, 40450, Selangor, Malaysia

⁴ Faculty of Pharmacy, Universitas Andalas, 25163, Padang, Indonesia

⁵ Department of Biology, Faculty of Mathematics and Sciences, Universitas Negeri Padang, 25171, Padang, Indonesia

⁶ Biomedical Science Program Study, Faculty of Medicine, Universitas Andalas, 25163, Padang, Indonesia

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Received: 18 May 2025; Revised: 5 Nov 2025; Accepted: 6 Dec 2025; Published: 7 Feb 2026.

BibTex Citation Data :

@article{JKSA73358,
    author = {Dessy Arisanty and Zolkapli Eshak and Fatma Sri Wahyuni and Afifatul Achyar and Salsabila Putri Khairani},
    title = {The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {29},
    number = {1},
    year = {2026},
    keywords = {miR-10b; Transfection; mimic miRNA; gene expression; HOXD10 gene; PI3K gene},
    abstract = { Breast cancer remains one of the leading causes of death in women worldwide, with high incidence and mortality rates. The abnormal expression of the small non-coding RNA, microRNA-10b (miR-10b), has been associated with breast cancer progression. miR-10b is involved in various biological processes, including proliferation, metastasis, and drug resistance. However, the underlying mechanism by which miR-10b contributes to breast cancer progression remains unclear. This study aimed to investigate the effect of miR-10b on the expression of HOXD10 and PI3K genes, as well as its impact on cell viability and proliferation in breast cancer cells. In this study, T47D breast cancer cells were transfected with mimic miR-10b using the FANA-oligo transfection method at concentrations of 1, 2.5, and 5 µM. Cell viability was assessed using the MTT assay, while HOXD10 and PI3K gene expression levels were measured using RT-qPCR and ELISA. Data were analysed using paired t-tests with SPSS, and results were considered statistically significant at  p  < 0.05. The results showed that mimic miR-10b induced a dose-dependent increase in cell proliferation. Notably, only HOXD10 gene expression was significantly affected by the transfection, while PI3K gene expression remained unchanged. Cells transfected with 5 µM miR-10b mimic exhibited the highest proliferation rate compared to non-transfected controls ( p  < 0.05). In conclusion, miR-10b promotes breast cancer cell proliferation by downregulating HOXD10 expression, independent of PI3K, suggesting it is a potential target for further investigation. },
   issn = {2597-9914},  doi = {10.14710/jksa.29.1.%p},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/73358}
}

Citation Format:

Abstract

Breast cancer remains one of the leading causes of death in women worldwide, with high incidence and mortality rates. The abnormal expression of the small non-coding RNA, microRNA-10b (miR-10b), has been associated with breast cancer progression. miR-10b is involved in various biological processes, including proliferation, metastasis, and drug resistance. However, the underlying mechanism by which miR-10b contributes to breast cancer progression remains unclear. This study aimed to investigate the effect of miR-10b on the expression of HOXD10 and PI3K genes, as well as its impact on cell viability and proliferation in breast cancer cells. In this study, T47D breast cancer cells were transfected with mimic miR-10b using the FANA-oligo transfection method at concentrations of 1, 2.5, and 5 µM. Cell viability was assessed using the MTT assay, while HOXD10 and PI3K gene expression levels were measured using RT-qPCR and ELISA. Data were analysed using paired t-tests with SPSS, and results were considered statistically significant at p < 0.05. The results showed that mimic miR-10b induced a dose-dependent increase in cell proliferation. Notably, only HOXD10 gene expression was significantly affected by the transfection, while PI3K gene expression remained unchanged. Cells transfected with 5 µM miR-10b mimic exhibited the highest proliferation rate compared to non-transfected controls (p < 0.05). In conclusion, miR-10b promotes breast cancer cell proliferation by downregulating HOXD10 expression, independent of PI3K, suggesting it is a potential target for further investigation.

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Keywords: miR-10b; Transfection; mimic miRNA; gene expression; HOXD10 gene; PI3K gene

Funding: PNBP Research Grant, Faculty of Medicine, Universitas Andalas under contract 78/BBPT/PNP/FK-UNAND-2019.

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Section: Research Articles

Language : EN

In Vol 29, No 1 (2026): Volume 29 Issue 1 Year 2026

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