skip to main content

Optimization of Enzymatic Bioremediation of Oil Contaminated Soil by Laccase from Marasmiellus palmovorus using Response Surface Methodology

*Agus Jatnika Effendi orcid  -  Institut Teknologi Bandung, Indonesia
Sri Harjati Suhardi  -  Institut Teknologi Bandung, Indonesia
Yollanda Chekti Kirana Arun Surya Widi  -  Institut Teknologi Bandung, Indonesia
Khomaini Hasan  -  Institut Teknologi Bandung, Indonesia
Dion Awfa  -  Institut Teknologi Sumatera, Indonesia
Rendana Saputra  -  Institut Teknologi Bandung, Indonesia

Citation Format:
Abstract

Crude oil contamination is considered highly toxic and poses a significant environmental problem. As an alternative, the laccase enzyme showed high performance for removing various toxic contaminants, particularly oil-contaminated soil (i.e., total petroleum hydrocarbon). However, previous studies mainly tested the performance of laccase under irrelevant environmental conditions (i.e., artificially contaminated soil) with a limited number of soil samples, which can lead to bias optimization results for assessing laccase performance. Two types of natural oil-contaminated soils were tested under various reaction times and various concentrations of laccase extracted from Marasmiellus palmovorus. In addition, response surface methodology was used to find the maximum degradation of total petroleum hydrocarbon (TPH). The maximum degradation of TPH from soil A and soil B were 48.57% and 54.1%, respectively. Moreover, the performance of the laccase enzyme for oil recovery was also tested, with the percentage of oil recovery being 9.89% and 10.1 % for soil A and soil B, respectively. SARA fraction analysis indicated that laccase enzyme preferentially degraded highly polar SARA fraction (i.e., asphaltene and resin). In general, the application of laccase for the enzymatic remediation of oil-contaminated soils was practical. Hence, the use of laccase for environmental application is still promising.

Fulltext View|Download
Keywords: Laccase, Enzyme, Total Petroleum Hydrocarbon, Bioremediation
Funding: Ministry of Research and Technology of Indonesia (BRIN)

Article Metrics:

  1. Aranda, E., Godoy, P., Reina, R., Badia-Fabregat, M., Rosell, M., Marco-Urrea, E., García-Romera, I., 2017. Isolation of Ascomycota fungi with the capability to transform PAHs: Insights into the biodegradation mechanisms of Penicillium oxalicum. International Biodeterioration and Biodegradation. 122, 141–150
  2. Arca-Ramos, A., Eibes, G., Moreira, M.T., Feijoo, G., Lema, J.M., 2012. Surfactant-assisted two-phase partitioning bioreactors for laccase-catalyzed degradation of anthracene. Process Biochemistry. 47, 1115–1121
  3. Arora, D.S., Gill, P.K., 2001. Effects of various media and supplements on laccase production by some white-rot fungi. Bioresource Technology. 77, 89–91
  4. Benguenab, A., & Chibani, A. (2020). Biodegradation of petroleum hydrocarbons by filamentous fungi (Aspergillus ustus and Purpureocillium lilacinum) isolated from used engine oil contaminated soil. Acta Ecologica Sinica
  5. Bhattacharya, M., Guchhait, S., Biswas, D., Singh, R., 2019. Evaluation of a microbial consortium for crude oil spill bioremediation and its potential uses in enhanced oil recovery. Biocatalysis and Agricultural Biotechnology. 18, 101034
  6. Chaudhary, D.K., Kim, J., 2019. New insights into bioremediation strategies for oil-contaminated soil in cold environments. International Biodeterioration and Biodegradation. 142, 58–72
  7. Concetta Tomei, M., Daugulis, A.J., 2013. Ex situ bioremediation of contaminated soils: An overview of conventional and innovative technologies. Critical Reviews in Environmental Science and Technology. 43, 2107–2139
  8. Coronel Vargas, G., Au, W.W., Izzotti, A., 2020. Public health issues from crude-oil production in the Ecuadorian Amazon territories. Science of the Total Environment. 719, 134647
  9. Eibes, G., Arca-Ramos, A., Feijoo, G., Lema, J.M., Moreira, M.T., 2015. Enzymatic technologies for remediation of hydrophobic organic pollutants in soil. Applied Microbiology and Biotechnology. 99, 8815–8829
  10. Joonaki, E., Buckman, J., Burgass, R., Tohidi, B., 2019. Water versus Asphaltenes; Liquid–Liquid and Solid–Liquid Molecular Interactions Unravel the Mechanisms behind an Improved Oil Recovery Methodology. International Journal Of Scientific Reports. 9, 1–13
  11. Karthick, A., Roy, B., Chattopadhyay, P., 2019. A review on the application of chemical surfactant and surfactant foam for remediation of petroleum oil contaminated soil. Journal of Environmental Management. 243, 187–205
  12. Kucharzyk, K.H., Benotti, M., Darlington, R., Lalgudi, R., 2018. Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads. Journal of Hazardous Materials. 357, 498–505
  13. Lin, T.C., Pan, P.T., Cheng, S.S., 2010. Ex situ bioremediation of oil-contaminated soil. Journal of Hazardous Materials. 176, 27–34
  14. Megharaj, M., Ramakrishnan, B., Venkateswarlu, K., Sethunathan, N., Naidu, R., 2011. Bioremediation approaches for organic pollutants: A critical perspective. Environment International. 37, 1362–1375
  15. Mohammadi-Sichani, M. M., Assadi, M. M., Farazmand, A., Kianirad, M., Ahadi, A. M., & Ghahderijani, H. H. (2017). Bioremediation of soil contaminated crude oil by Agaricomycetes. Journal of Environmental Health Science and Engineering, 15(1), 0–6
  16. Mori, T., Watanabe, M., Taura, H., Kuno, T., Kamei, I., Kondo, R., 2015. Degradation of chlorinated dioxins and polycyclic aromatic hydrocarbons (PAHs) and remediation of PAH-contaminated soil by the entomopathogenic fungus, Cordyceps militaris. Journal of Environmental Chemical Engineering. 3, 2317–2322
  17. Polyak, Y.M., Bakina, L.G., Chugunova, M. V., Mayachkina, N. V., Gerasimov, A.O., Bure, V.M., 2018. Effect of remediation strategies on biological activity of oil-contaminated soil - A field study. Int. International Biodeterioration and Biodegradation. 126, 57–68
  18. Quintella, C.M., Mata, A.M.T., Lima, L.C.P., 2019. Overview of bioremediation with technology assessment and emphasis on fungal bioremediation of oil contaminated soils. Journal of Environmental Management. 241, 156–166
  19. Rahmani, H., Lakzian, A., Karimi, A., Halajnia, A., 2020. Efficient removal of 2,4-dinitrophenol from synthetic wastewater and contaminated soil samples using free and immobilized laccases. Journal of Environmental Management. 256, 109740
  20. Rayu, S., Karpouzas, D.G., Singh, B.K., 2012. Emerging technologies in bioremediation: Constraints and opportunities. Biodegradation 23, 917–926
  21. Roshandel, F., Saadatmand, S., Iranbakhsh, A., & Ardebili, Z. O. (2021). Mycoremediation of oil contaminant by Pleurotus florida (P.Kumm) in liquid culture. Fungal Biology, xxxx. https://doi.org/10.1016/j.funbio.2021.04.002
  22. Strong, P.J., ClauReferences
  23. Aranda, E., Godoy, P., Reina, R., Badia-Fabregat, M., Rosell, M., Marco-Urrea, E., García-Romera, I., 2017. Isolation of Ascomycota fungi with the capability to transform PAHs: Insights into the biodegradation mechanisms of Penicillium oxalicum. International Biodeterioration and Biodegradation. 122, 141–150
  24. Arca-Ramos, A., Eibes, G., Moreira, M.T., Feijoo, G., Lema, J.M., 2012. Surfactant-assisted two-phase partitioning bioreactors for laccase-catalyzed degradation of anthracene. Process Biochemistry. 47, 1115–1121
  25. Arora, D.S., Gill, P.K., 2001. Effects of various media and supplements on laccase production by some white-rot fungi. Bioresource Technology. 77, 89–91
  26. Benguenab, A., & Chibani, A. (2020). Biodegradation of petroleum hydrocarbons by filamentous fungi (Aspergillus ustus and Purpureocillium lilacinum) isolated from used engine oil contaminated soil. Acta Ecologica Sinica
  27. Bhattacharya, M., Guchhait, S., Biswas, D., Singh, R., 2019. Evaluation of a microbial consortium for crude oil spill bioremediation and its potential uses in enhanced oil recovery. Biocatalysis and Agricultural Biotechnology. 18, 101034
  28. Chaudhary, D.K., Kim, J., 2019. New insights into bioremediation strategies for oil-contaminated soil in cold environments. International Biodeterioration and Biodegradation. 142, 58–72
  29. Concetta Tomei, M., Daugulis, A.J., 2013. Ex situ bioremediation of contaminated soils: An overview of conventional and innovative technologies. Critical Reviews in Environmental Science and Technology. 43, 2107–2139
  30. Coronel Vargas, G., Au, W.W., Izzotti, A., 2020. Public health issues from crude-oil production in the Ecuadorian Amazon territories. Science of the Total Environment. 719, 134647
  31. Eibes, G., Arca-Ramos, A., Feijoo, G., Lema, J.M., Moreira, M.T., 2015. Enzymatic technologies for remediation of hydrophobic organic pollutants in soil. Applied Microbiology and Biotechnology. 99, 8815–8829
  32. Joonaki, E., Buckman, J., Burgass, R., Tohidi, B., 2019. Water versus Asphaltenes; Liquid–Liquid and Solid–Liquid Molecular Interactions Unravel the Mechanisms behind an Improved Oil Recovery Methodology. International Journal Of Scientific Reports. 9, 1–13
  33. Karthick, A., Roy, B., Chattopadhyay, P., 2019. A review on the application of chemical surfactant and surfactant foam for remediation of petroleum oil contaminated soil. Journal of Environmental Management. 243, 187–205
  34. Kucharzyk, K.H., Benotti, M., Darlington, R., Lalgudi, R., 2018. Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads. Journal of Hazardous Materials. 357, 498–505
  35. Lin, T.C., Pan, P.T., Cheng, S.S., 2010. Ex situ bioremediation of oil-contaminated soil. Journal of Hazardous Materials. 176, 27–34
  36. Megharaj, M., Ramakrishnan, B., Venkateswarlu, K., Sethunathan, N., Naidu, R., 2011. Bioremediation approaches for organic pollutants: A critical perspective. Environment International. 37, 1362–1375
  37. Mohammadi-Sichani, M. M., Assadi, M. M., Farazmand, A., Kianirad, M., Ahadi, A. M., & Ghahderijani, H. H. (2017). Bioremediation of soil contaminated crude oil by Agaricomycetes. Journal of Environmental Health Science and Engineering, 15(1), 0–6
  38. Mori, T., Watanabe, M., Taura, H., Kuno, T., Kamei, I., Kondo, R., 2015. Degradation of chlorinated dioxins and polycyclic aromatic hydrocarbons (PAHs) and remediation of PAH-contaminated soil by the entomopathogenic fungus, Cordyceps militaris. Journal of Environmental Chemical Engineering. 3, 2317–2322
  39. Polyak, Y.M., Bakina, L.G., Chugunova, M. V., Mayachkina, N. V., Gerasimov, A.O., Bure, V.M., 2018. Effect of remediation strategies on biological activity of oil-contaminated soil - A field study. Int. International Biodeterioration and Biodegradation. 126, 57–68
  40. Quintella, C.M., Mata, A.M.T., Lima, L.C.P., 2019. Overview of bioremediation with technology assessment and emphasis on fungal bioremediation of oil contaminated soils. Journal of Environmental Management. 241, 156–166
  41. Rahmani, H., Lakzian, A., Karimi, A., Halajnia, A., 2020. Efficient removal of 2,4-dinitrophenol from synthetic wastewater and contaminated soil samples using free and immobilized laccases. Journal of Environmental Management. 256, 109740
  42. Rayu, S., Karpouzas, D.G., Singh, B.K., 2012. Emerging technologies in bioremediation: Constraints and opportunities. Biodegradation 23, 917–926
  43. Roshandel, F., Saadatmand, S., Iranbakhsh, A., & Ardebili, Z. O. (2021). Mycoremediation of oil contaminant by Pleurotus florida (P.Kumm) in liquid culture. Fungal Biology, xxxx. https://doi.org/10.1016/j.funbio.2021.04.002
  44. Strong, P.J., Claus, H., 2011. Laccase: A review of its past and its future in bioremediation. Crit. Rev. Environ. Sci. Technol. 41, 373–434. https://doi.org/10.1080/10643380902945706
  45. Sun, X., Zhang, Y., Cui, G., Duan, X., Zhao, C., 2014. Feasibility study of enhanced foamy oil recovery of the Orinoco Belt using natural gas. Journal of Petroleum Science and Engineering. 122, 94–107
  46. Wu, Y., Teng, Y., Li, Z., Liao, X., Luo, Y., 2008. Potential role of polycyclic aromatic hydrocarbons (PAHs) oxidation by fungal laccase in the remediation of an aged contaminated soil. Soil Biology and Biochemistry. 40, 789–796
  47. Xu, Y., Lu, M., 2011. Microbially enhanced oil recovery at simulated reservoir conditions by use of engineered bacteria. Journal of Petroleum Science and Engineering. 78, 233–238
  48. Zhang, Y., Lin, D.F., Hao, J., Zhao, Z.H., Zhang, Y.J., 2020. The crucial role of bacterial laccases in the bioremediation of petroleum hydrocarbons. World Journal of Microbiology and Biotechnology. 36, 1–10

Last update:

No citation recorded.

Last update:

No citation recorded.