DOI: https://doi.org/10.14710/ijfst.15.1.80-85

STATUS HEMATOLOGI DAN RESPON IMUN IKAN KOI (Cyprinus carpio) YANG TERINFEKSI Myxobolus sp. DENGAN TREATMENT DIMILIN (Hematological Status and Immune Response of Koi Fish (Cyprinus carpio) Infected by Myxobolus sp. With Treatment of Dimilin)

*feri Setiawan  -  Brawijaya University, Indonesia
Uun Yanuhar  -  Brawijaya University, Indonesia
Andi Kurniawan  -  Brawijaya University, Indonesia

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Abstract

Koi merupakan ikan hias favorit di pasar nasional dan internasional. Proses budidaya untuk menghasilkan koi dengan kualitas terbaik masih menjadi kendala, salah satunya adanya infeksi parasit Myxobolus sp. yang dapat menyebabkan kematian massal dalam waktu singkat. Myxobolus yang menyerang jaringan ikan dapat menyebabkan kerusakan prah pada jaringan. Banyak cara dilakukan oleh pembudidaya Ikan Koi untuk menghindari infeksi Myxobolus, yaitu dengan pengobatan secara alami dan menggunakan bahan kimia. Penelitian ini bertujuan untuk mengetahui efek hematologi dan respon imun pada Ikan Koi yang terinfeksi parasit Myxobolus dengan pengobatan dimilin. Metode yang digunakan adalah metode eksperimental dengan perlakuan kontrol ikan sehat (A), Ikan koi yang terinfeksi oleh Myxobolus sp. tanpa perlakuan dimilin (B), ikan koi terinfeksi Myxobolus sp dengan perlakuan dimilin 0,02 mg (C), dan ikan yang terinfeksi Myxobolus sp dengan perlakuan dimilin 0,01 mg (D). Pengambilan darah ikan dilakukan dengan metode punksi pembuluh darah bagian caudal dan dilakukan pengamatan hematologi, serta dilakukan pengamatan imunohistokima dengan melihat persentase Diaminobenzidine (DAB) pada ikan. Hasil rata-rata pada perhitungan hematologi leukosit dan eritrosit tertinggi  ditemukan pada ikan yang terinfeksi Myxobolus sp tanpa perlakuan dimilin (B), dengan eritrosit 2.913.334 sel / mm3 dan leukosit 197.184 sel / mm3. Hasil respon imun berdasarkan pengamatan imunohistokimia didapatkan nilai DAB perlakuan A adalah 16%, nilai DAB perlakuan B 42,7%, nilai DAB perlakuan C 25,6%, dan nilai DAB perlakuan D 31,4%. Kesimpulan penelitian ini adalah pemberian dimilin dapat mempengaruhi respon hematologi dan dapat mempengaruhi respon imun pada ikan yang dapat ditunjukkan dengan Interleukin-6 pada ikan dengan hasil DAB immunoratio.

 

Koi is a favorite ornamental fish in the national and international markets. Cultivation process to produce koi with the best quality is still have problem, one of the problem is parasitic infection of Myxobolus sp. which can cause mass death in a short time. Myxobolus attacks fish tissue can cause severe damage to tissue. Many ways were used by koi fish farmers to avoid Myxobolus infection which  with natural treatment and using chemicals drug. This study aims to determine the hematological effects and immune respons in the koi fish infected with the Myxobolus and treated with dimilin. Experimental method was used in this research with control treatment of healthy fish (A), koi fish infected by Myxobolus sp. without treatment (B), koi fish infected by Myxobolus sp with dimilin treatment 0.02 mg (C), and koi fish infected by Myxobolus sp with dimilin treatment 0.01 mg (D). fish Blood samples were collected with puncture of caudal vessel method and then haematological observations to blood samples, and immunohistokima observations by looking percentage of Diaminobenzidine (DAB) in fish. Highest hematological Results of leukocytes and erythrocytes were found in treatment B, with erythrocytes result 2,913,334 cells / mm3 and leukocyte 197,184 cells / mm3. Immune response result based on immunohistochemical observations with DAB value of treatment A is 16%, treatment B is 42.7%, treatment C is 25.6%, and treatment D is 31.4%. Conclusion in this study that dimilin treatment can affect hematological response and immune response in fish which can be shown with Interleukin-6 in fish with DAB immunoratio results. 

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Keywords: Cyprinus carpio; Myxobolus sp.; Interleukin-6; Hematologi; Eritrosit; Leukosit; Dimilin

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  1. Ashley, N. T., Weil, Z. M., dan Nelson, R. J. 2012. Inflammation: Mechanisms, Costs, and Natural Variation. Annual Review of Ecology, Evolution, and Systematics, 43(1), 385–406. DOI: 10.1146/annurev-ecolsys-040212-092530
  2. Bachtiar, I.Y. dan Lentera, T., 2002. Mencemerlangkan Warna Koi. AgroMedia
  3. Bachtiar, Y. 2002. Pembesaran Ikan Mas Di Kolam Air Deras. Jakarta: Agromedia Pustaka
  4. Blaxhall, P.C. and Daisley, K.W., 1973. Routine Haematological Methods for Use With Fish Blood. Journal of fish biology, 5(6), pp.771-781. DOI: 10.1111/j.1095-8649.1973.tb04510.x
  5. Clauss, T.M., Dove, A.D.M., Arnold, J.E., 2008. Hematologic Disorders of Fish. Vet. Clin.North.Am. Exot. Anim. Pract. 11, 445–462. DOI: 10.1016/j.cvex.2008.03.007
  6. Davinia, M., dan S. A. Mackenzie. 2011. Is There A Direct Role For Erythrocytes in The Immune Response? Veterinary Research, 42(1), 89. DOI: 10.1186/1297-9716-42-89
  7. Davis, A. K., D. L. Maney, and J. C. Maerz. 2008. The Use Of Leukocyte Profiles To Measure Stress In Vertebrates: A Review For Ecologists. Functional Ecology, 22(5), 760-772. DOI: 10.1111/j.1365-2435.2008.01467.x
  8. Djajadiredja, R., A. Panjaitan, A. Rukyani, D. Sarono, Satyani, and H. Supriyadi. 1982. Fish Quarantine and Fish Diseases in Southeast Asia : Report of A Workshop Held In Jakarta. Indonesia, 7-10 P. 79 P. : 21pp,
  9. Direktorat Jenderal Perikanan Budidaya. 2018. Laporan Kinerja (LKj) Direktorat Jenderal Perikanan Budidaya Tahun 2018
  10. Eisler, R. 2007. Eisler’s Encyclopedia of Environmentally Hazardous Priority Chemicals. Elsevier Science
  11. Fischer, S. A., and Hall, L. W. 1992. Environmental Concentrations And Aquatic Toxicity Data On Diflubenzuron (Dimilin). Critical Reviews In Toxicology, 22(1), 45-79. DOI: 10.3109/10408449209145321
  12. Hoole, D, D. Bucke, P. Burgess, and I. Wellby. 2001. Diseases of carp and other cyprinid fishes. Oxford: Fishing News Books
  13. Hoole, D., Bucke, D., Burgess, P., & Wellby, I. 2001. Diseases Of Carp And Other Cyprinid Fishes. Oxford: Fishing News Books
  14. Insivitawati, E., Mahasri, G. dan Kusnoto, K., 2015. Gambaran Darah dan Histopatologi Insang, Usus Dan Otak Ikan Koi (Cyprinus carpio) yang Diinfeksi Spora Myxobolus koi secara Oral [Haematology and Histopatology of Gills, Intestine And Brain Koi Fish (Cyprinus carpio) Myxobolus koi Orally Infected]. Jurnal Ilmiah Perikanan dan Kelautan, 7(2), pp.225-234
  15. Kent, M. L., K. B. Andree, J. L. Bartholomew, M. El‐Matbouli, S. S. Desser, R. H. Devlin, S. W. Feist. 2001. Recent Advances in Our Knowledge of the Myxozoa. Journal of Eukaryotic Microbiology 48, no. 4: 395-413. DOI: 10.1111/j.1550-7408.2001.tb00173.x
  16. Kusrini, E., Cindelaras, S. dan Prasetio, A.B., 2015. Pengembangan Budidaya Ikan Hias Koi (Cyprinus Carpio) Lokal Di Balai Penelitian Dan Pengembangan Budidaya Ikan Hias Depok. Media Akuakultur, 10(2), pp.71-78
  17. Liu, X., Hua, C., Zhang, Q., Zhao, Y., Zhang, D. and Zhang, J., 2017. Myxobolus taibaiensis sp. (Myxozoa: Myxosporea) Infecting the Intestinal Wall of Common Carp Cyprinus carpio Linnaeus in China. Folia parasitologica, 64, p.1. DOI: 10.14411/fp.2017.001
  18. Mahasri, G. 2017. Development of Spore Protein Of Myxobolus Koi As An Immunostimulant For Prevent Of Myxobolusis On Gold Fish (Cyprinus Carpio Linn) By Oral Immunisation. 2nd Int. Conf. Trop. Coast. Reg. Eco Dev. DOI: 10.1088/1755-1315/55/1/012009
  19. Mariyono, Puspitasari dan Sutomo. 2000. Tehnik Uji Ketahanan Bibit Ikan Nila Dan Nila Terhadap Bakteri Aeromonas Hydrophila Dengan Berbagai Kepadatan. Buletin Tehnik Pertanian, 5(II) : 77-78
  20. Morera, D., N. Roher, L. Ribas, J. C. Balasch, DoNate, C., Callol, A. 2011. Rnaseq Reveals An Integrated Immune Response In Nucleated Erythrocytes. Plos One, 6(10), E26998. DOI: 10.1371/journal.pone.0026998
  21. Moyle, P.B And J.J Cech. 2004. Fish An Introduction To Ichthyology Fifth Edition. Prentice Hall: New Jersey
  22. Oge, S. 2002. Cheomotheraphy For Parasites Of Freshwater Fish. J. Turkish Parazitol, Vol. 26, Pp. 113–118
  23. Pandey, D.G., 2013. Treatment for Certain Parasitic Diseases of Fishes. Universal Journal of Pharmacy, 2(02), pp.1-3
  24. Prihartini, N. C. And Alfiyah. 2017. Myxosporeasis pada Ikan Koi (Cyprinus Carpio). Samakia J. Ilmu Perikan., Vol. 8, No. 1, Pp. 6–10
  25. Roberts, R. J. 2012. Fish Pathology. John Wiley & Sons
  26. Rukyani, A. 1990. Histopathological Changes In The Gill Of Common Carp (Cyprinus carpio L.) Infected With The Myxosporean Parasite Myxobolus Koi Kudo,1920,. Asian Fish. Sci. 3, Pp. 337–341
  27. Soltani-Mazouni, N. 1994. Effects of Ingested Diflubenzuron on Ovarian Development During the Sexual Maturation of Mealworms. Tissue And Cell, 26(3), 439–445. DOI: 10.1016/0040-8166(94)90027-2
  28. Steckler, N. dan R. P. E. Yanong. 2012. Argulus ( Fish Louse ) Infections In Fish. Univ. Florida IFAS Ext., Pp. 1–5,
  29. Supriyadi , H. dan T. Lentera. 2004.Membuat Ikan Hias Tampil Sehat & Prima. Jakarta: Agromedia
  30. Svobodova, Z., and B. Vykusova. 1991. Diagnostics, Prevention And Therapy of Fish Diseases and Intoxications. Manual for International Training Course on Fresh-Water Fish Diseases and Intoxications (in Czech). Research Institute of Fish Culture and Hydrobiology, Vodnany
  31. Syawal H, Syaifriadiman, S. Hidayah. 2008. Pemberian Ekstrak Kayu Siwak (Salvadora persica L.) Untuk Meningkatkan Kekebalan Ikan Mas (Cyprinus carpio L.) yang Dipelihara dalam Keramba. ISSN : 1412-033X Januari 2008
  32. Wang, T., dan C. J. Secombes. (2009). Identification and Expression Analysis of Two Fish-Specific IL-6 Cytokine Family Members, the Ciliary Neurotrophic Factor (CNTF)-Like and M17 Genes, in Rainbow Trout Oncorhynchus Mykiss. Molecular Immunology, 46(11-12), 2290-2298. DOI: 10.1016/j.molimm.2009.04.003
  33. Witeska, M. 2005. Stress In Fish-Hematological and Immunological Effects Of Heavy Metals. Electronic Journal of Ichthyology, 1(1), 35-41
  34. Zou, J. Dan C. Secombes. 2016. The Function Of Fish Cytokines, Biology (Basel)., Vol. 5, No. 2, P. 23. DOI: 10.3390/biology5020023

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