Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

*Astrilia Damayanti orcid scopus  -  Department of Chemical Engineering, Universitas Negeri Semarang, Kampus Sekaran, Gunungpati, Semarang,, Indonesia
Sarto Sarto  -  Department of Chemical Engineering Universitas Gadjah Mada, Yogyakarta, Indonesia
Wahyudi Budi Sediawan  -  Department of Chemical Engineering Universitas Gadjah Mada, Yogyakarta, Indonesia
Received: 16 Jun 2019; Revised: 17 Nov 2019; Accepted: 16 Jan 2020; Published: 18 Feb 2020; Available online: 15 Feb 2020.
Open Access Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract
Biohydrogen production via dark fermentation is a prospective renewable energy technology. In the process, reused of immobilized mixed culture is very important as their activities greatly influencehydrogen production. The aim of this work was to evaluate the reuse of alginate beads affecting the biohydrogen production for 45 days. This study in batch reactor were performed using glucose 10 M as substrate, alginate and activated carbon as immobilization matrix materials, chicken eggshell as buffer, and cow dung biodigester as mixed culture. Hydrogen and pH on fermentation product are investigated by gas chromatography (GC) technique and pH meter, respectively. The colony diameter on immobilized and co-immobilized mixed culture was observed using optical microscope and colony diameter was measured using Image-Pro Plus Software v4.5.0.29. The surface morphology of immobilization and co-immobilization beads were determined using scanning electron microscope (SEM). The results showed that the colonies growth observed using optical microscopy or SEM was apparent only in the immobilization of mixed culture. The average growth and diameter of colonies per day were 90 colonies and 0.025 mm, respectively. The weight of beads and pH during the 45-day fermentation process for bead immobilization of mixed culture were 1.32–1.95 g and 6.25–6.62, correspondingly, meanwhile, the co-immobilizations of the mixed culture were 1.735–2.21g and 6.25–6.61, respectively. In addition, the average hydrogen volume of glucose fermented using an eggshell buffer and reusing the immobilization and co-immobilization beads was 18.91 mL for 15 cycles.©2020. CBIORE-IJRED. All rights reserved
Keywords: biohydrogen; reused beads; immobilization; mixed culture; batch

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Section: Original Research Article
Language: EN
In Vol 9, No 1 (2020): February 2020
Statistics: 1051 626
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  1. Akil, K. and Jayanthi, S. (2015), The Biohydrogen Potential of Distillery Wastewater by Dark Fermentation in an Anaerobic Sequencing Batch Reactor, International Journal of Green Energy, 11(1), 28–39
  2. Alonso, S., Rendueles, M. and Díaz, M. (2015), A Novel Approach to Monitor Stress-Induced Physiological Responses in Immobilized Microorganisms, Applied Microbiology and Biotechnology, 99 (8), 3573–3583
  3. Cai, J. and Wang, G. (2016), Comparison of Different Pre-Treatment Methods for Enriching Hydrogen-Producing Bacteria from Intertidal Sludge, International Journal of Green Energy, 13(3), 292–297
  4. Cheong, D.-Y., Hansen, C.. and Stevens, D. (2006), Production of Bio-Hydrogen by Mesophilic Anaerobic Fermentation in an Acid-Phase Sequencing Batch Reactor, Biotechnology and Bioengineering,96,421–432
  5. Covarrubias, S.A., De-Bashan, L.E., Moreno, M. and Bashan, Y. (2012), Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae, Applied Microbiology and Biotechnology,93(6), 2669–80
  6. Damayanti, A., Sarto, Sediawan, W.B. and Syamsiah, S. (2018), Performance analysis of immobilized and co-immobilized enriched-mixed culture for hydrogen production, Journal of Mechanical Engineering and Sciences, 12(1), 3515–3528
  7. Damayanti, A., Sarto, Syamsiah, S. and Sediawan, W.B. (2017), The Influence of Chicken Eggshell Powder As A Buffer on Biohydrogen Production from Rotten Orange (Citrus Nobilis Var. Microcarpa) with Immobilized Mixed Culture, The 3rd International, Vol. 1855, AIP Publishing, In Conference on Engineering, Technology, and Industrial Application (ICETIA), Surakarta, Indonesia, pp. 070006-1–7
  8. Das, D. (2009), Advances in biohydrogen production processes : An approach towards commercialization, International Journal of Hydrogen Energy, 34(17), 7349–7357
  9. Davila-Vazquez, G., de León-Rodríguez, A., Alatriste-Mondragón, F. and Razo-Flores, E. (2011), The buffer composition impacts the hydrogen production and the microbial community composition in non-axenic cultures, Biomass and Bioenergy, 35(7),3174–3181
  10. Deublein, D. and Steinhauser, A. (2008), Biogas from Waste and Renewable Resources : An Introduction, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. pp.114
  11. Dzionek, A., Wojcieszyńska, D. and Guzik, U. (2016), Natural carriers in bioremediation: A review, Electronic Journal of Biotechnology, 23, 28–36
  12. Hassan, A.F., Abdel-Mohsen, A.M. and Fouda, M.M.G. (2014), Comparative study of calcium alginate, activated carbon, and their composite beads on methylene blue adsorption, Carbohydrate Polymers, 102, 192–8
  13. Hu, B., Liu, Y., Chi, Z. and Chen, S. (2007), Biological Hydrogen Production Via Bacteria Immobilized in Calcium Alginate Gel Beads, Biological Engineering, 1, 25–37
  14. Jamali, N.S., Jahim, J.M. and Isahak, W.N.R.W. (2016), Biofilm formation on granular activated carbon in xylose and glucose mixture for thermophilic biohydrogen production, International Journal of Hydrogen Energy, 41(46), 21617–21627
  15. Kumar, A., Jain, S.R., Sharma, C.B., Joshi, A.P. and Kalia, V.C. (1995), Increased H2 production microorganisms by immobilized, World Journal of Mcrobiology & Biotechnology, 11, 156–159
  16. Lee, K.Y. and Mooney, D.J. (2012), Alginate: Properties and Biomedical Applications, Progress in Polymer Science, 37(1), 106–126
  17. Luo, G., Xie, L., Zou, Z., Wang, W. and Zhou, Q. (2010), Exploring optimal conditions for thermophilic fermentative hydrogen production from cassava stillage, International Journal of Hydrogen Energy, 35(12), 6161–6169
  18. Mesran, M.H., Mamat, S., Pang, Y.R., Hong, T.Y., Muneera-Z, Ghazali, N.F.M., Ali, Md, A., et al. (2014), Preliminary Studies on Immobilized Cells-Based Microbial Fuel Cell System on Its Power Generation Performance, Journal of Asian Scientific Research, 4 (8), 428–435
  19. Muñoz-Páez, K.M., Poggi-Varaldo, H.M., García-Mena, J., Ponce-Noyola, M.T., Ramos-Valdivia, A.C., Barrera-Cortés, J., Robles-González, I. V., et al. (2014), Cheese whey as substrate of batch hydrogen production: Effect of temperature and addition of buffer, Waste Management and Research, 32(5), 434–440
  20. Neunzehn, J., Szuwart, T. and Wiesmann, H.-P. (2015), Eggshells as natural calcium carbonate source in combination with hyaluronan as beneficial additives for bone graft materials , an in vitro study, Head & Face Medicine, 11:12 (12), 1–10
  21. Ng, F.L., Phang, S.M., Periasamy, V., Yunus, K. and Fisher, A.C. (2017), Enhancement of Power Output by using Alginate Immobilized Algae in Biophotovoltaic Devices, Scientific Reports, Springer US, 7(1), 1–8
  22. Penniston, J. and Kana, E.B.G. (2018), Impact of medium pH regulation on biohydrogen production in dark fermentation process using suspended and immobilized microbial cells, Biotechnology and Biotechnological Equipment, 32(1), 204–212
  23. Reyhani, S.. and Zilouei, H. (2013), Enhanced Biohydrogen Production From Wastewater And The Influence Of Operating Parameters, International Journal of Green Energy, 10, 321–336
  24. Saripan, A.F. and Reungsang, A. (2014), Thermophilic Fermentative Biohydrogen Production From Xylan by Anaerobic Mixed Cultures in Elephant Dung, International Journal of Green Energy, 12(9), 900–907
  25. Sekoai, P.T., Awosusi, A.A., Yoro, K.O., Singo, M., Oloye, O., Ayeni, A.O., Bodunrin, M., et al. (2017), Microbial cell immobilization in biohydrogen production: a short overview, Critical Reviews in Biotechnology, 38(2), 1–15
  26. Sekoai, P.T., Yoro, K.O. and Daramola, M.O. (2016), Batch Fermentative Biohydrogen Production Process Using Immobilized Anaerobic Sludge from Organic Solid Waste, Environments, 3(4), 38
  27. Shoichet, M.S., Li, R.H., White, M.L. and Winn, S.R. (1996), Stability of Hydrogels Used in Cell Encapsulation : An In Vitro Comparison of Alginate and Agarose, Biotechnology and Bioengineering, 50, 374–381
  28. Siahpush, A.R., Lin, J.E. and Wang, H.Y. (1992), Effect of Adsorbents on Degradation of Toxic Organic Compounds by Coimmobilized Systems, Biotechnology and Bioengineering, 39, 619–628
  29. Singh, L. and Wahid, Z.A. (2014), Methods for enhancing bio-hydrogen production from biological process: A review, Journal of Industrial and Engineering Chemistry, 21, 70–80
  30. Sivagurunathan, P., Sen, B. and Lin, C.Y. (2014), Batch fermentative hydrogen production by enriched mixed culture: Combination strategy and their microbial composition., Journal of Bioscience and Bioengineering, 117(2), 222–8
  31. Smidsrød, O. and Skjåk-Braek, G. (1990), Alginate as Immobilization Matrix for Cells, Trend Biotechnol, 8(3),71–8
  32. Wang, S., Ma, Z., Zhang, T., Bao, M. and Su, H. (2017), Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch, Frontiers of Chemical Science and Engineering, 11(1), 100–106
  33. Wu, S.Y., Lin, C.N., Chang, J.S., Lee, K.S. and Lin, P.J. (2002), Microbial hydrogen production with immobilized sewage sludge., Biotechnology Progress, 18 (5), 921–926
  34. Wu, X., Yao, W. and Zhu, J. (2010), Effect of pH on Continuous Biohydrogen Production From Liquid Swine Manure with Glucose Supplement Using An Anaerobic Sequencing Batch Reactor, International Journal of Hydrogen Energy, 35(13), 6592–6599
  35. Zhang, C., Kang, X., Liang, N. and Abdullah, A. (2017), Improvement of Biohydrogen Production from Dark Fermentation by Cocultures and Activated Carbon Immobilization, Energy and Fuels, 31(11), 12217–12222
  36. Zhu, H., Parker, W., Basnar, R., Proracki, A., Falletta, P., Béland, M. and Seto, P. (2009), Buffer requirements for enhanced hydrogen production in acidogenic digestion of food wastes, Bioresource Technology, 100(21), 5097–5102
  37. Zohar-Perez, C., Chet, I. and Nussinovitch, A. (2004), Unexpected distribution of immobilized microorganisms within alginate beads, Biotechnology and Bioengineering, 88(5), 671–674

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