Batch and Fed-Batch Fermentation System on Ethanol Production from Whey using Kluyveromyces marxianus
Nowadays reserve of fossil fuel has gradually depleted. This condition forces many researchers to find energy alternatives which is renewable and sustainable in the future. Ethanol derived from cheese industrial waste (whey) using fermentation process can be a new perspective in order to secure both energy and environment. The aim of this study was to compare the operation modes (batch and fed-batch) of fermentation system on ethanol production from whey using Kluyveromyces marxianus. The result showed that the fermentation process for ethanol production by fed-batch system was higher at some point of parameters compared with batch system. Growth rate and ethanol yield (YP/S) of fed-batch fermentation were 0.122/h and 0.21 gP/gS respectively; growth rate and ethanol yield (YP/S) of batch fermentation were 0.107/h, and 0.12 g ethanol/g substrate, respectively. Based on the data of biomass and ethanol concentrations, the fermentation process for ethanol production by fed-batch system were higher at some point of parameters compared to batch system. Periodic substrate addition performed on fed-batch system leads the yeast growth in low substrate concentrations and consequently increasing their activity and ethanol productivity.
Keywords: batch; ethanol; fed-batch; fermentation;Kluyveromyces marxianus, whey
- Ariyanti, D. & Hadiyanto (2013) Ethanol production from whey by Kluyveromyces marxianus in batch fermentation system: kinetics parameters estimation. Bulletin of Chemical Engineering and Catalysis, 7(3), 179-184.
- Becerra, M. & Siso, M.I.G. (2006) Yeast β-Galaktosidase in solid-state fermentations. Journal of Enzyme and Microbial Technology, 19, 39-44.
- Cheng, N. G., Hasan, M., Kumoro, A. C., Ling, C. F. & Tham, M. (2009) Production of ethanol by fed-batch fermentation. Journal Science and Technology Pertanika, 17(2), 399-408.
- Foda, M. I., Joun, H. & Li, Y. (2010) Study the Suitability of cheese whey for biobutanol production by ClostridiaI. Journal of American Science, 39-46.
- Goshima, T., Tsuji, M., Inoue, H., Yano, S., Hoshino, T. & Matsushika, A. 2013. Bioethanol production from lignocellulosic biomass by a novel Kluyveromyces marxianus strain. Bioscience, Biotechnology and Biochemical, 77(7), 1505-1510.
- Guimarães, P.M.R., Teixeira, J.A. & Domingues, L. 2010, Fermentation of lactose to bio-ethanol by yeasts as part of integrated solutions for the valorization of cheese whey. Biotechnology Advances, 28, 375–384.
- Kourkoutas, Y., Dimitropoulou, S., Kanellaki, M., Marchant, R., Nigam, P., Banat, I. M. & Koutinas, A.A. (2002) High Temperature alcoholic fermentation of whey using Kluyveromyces marxianus IMB3 yeast immobilized on delignified cellulosic material. Journal of Bioresources Technology, 82, 177-181.
- Lukondeh, T., Ashbolt, N.J. & Rogers, P.L. (2005) Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium. Journal of Industrial Microbiology and Biotechnology, 32(7), 284-288.
- Rech R. & Ayub, M.A.Z. (2006) Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey. Brazilian Journal of Chemical Engineering, 23 (04), 435 - 442.
- Rech, R. & Ayub, M.A.Z. (2007) Simplified feeding strategies for fed-batch cultivation of Kluyveromyces marxianus in cheese whey. Journal of Elsevier, 42 (5), 873–877.
- Saarela, U., Leiviska, K. & Juuso, E. (2003) Modelling of a fed-batch fermentation process. Report A No. 21, June 2003. Control Engineering Laboratory, University of Oulu Finland.
- Shahani, K.M. & Friend, B.A. (1980) Fuel Alcohol Production from whey and grain mixtures. Department of Food Science and Technology University of Nebraska Lincoln. American Chemical Society Division.
- Toyoda, T. & Kazuhisa O. (2008). Production of ethanol from lactose by Kluyveromyces lactis NBRC 1903. Thammasat International Journal of Science and Technology, 13, 30-35.
- Zafar, S. & Owais, M. (2005) Ethanol production from crude whey by Kluyveromyces marxianus. Biochemisal Engineering Journal, 27, 295-298.
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to International Journal of Renewable Energy Development and Center of Biomass and Renewable Energy, Department of Chemical Engineering Diponegoro University as publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from International Journal of Renewable Energy Development and Center of Biomass and Renewable Energy, Department of Chemical Engineering Diponegoro University.
International Journal of Renewable Energy Development and Center of Biomass and Renewable Energy, Department of Chemical Engineering Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the International Journal of Renewable Energy Development are sole and exclusive responsibility of their respective authors and advertisers.