Pengaruh Garam Monovalen (NaCl dan KCl) dan Divalen (CaCl2 dan MgCl2) Terhadap Aktivitas Protease Ekstraseluler Bakteri Halofilik Isolat Bittern Tambak Garam Madura

DOI: https://doi.org/10.14710/jksa.20.1.37-41
License URL: http://creativecommons.org/licenses/by-sa/4.0/

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Article Info
Published: 01-04-2017
Section: Research Articles
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Bakteri halofilik mampu tumbuh dan bertahan hidup pada lingkungan dengan kadar garam tinggi, seperti dalam air pekatan sisa tambak garam (bittern). Bakteri halofilik dapat menghasilkan enzim protease yang tahan terhadap garam (halostabil). Protease halostabil dapat dimanfaatkan pada proses degradasi limbah pada kawasan yang mengandung garam. Penelitian ini bertujuan untuk memperoleh protease ekstraseluler bakteri halofilik isolat bittern tambak garam Madura dan menentukan pengaruh garam monovalen (NaCl dan KCl) dan divalen (CaCl2 dan MgCl2) terhadap aktivitas dan profil aktivitas protease halostabil. Berdasarkan penelitian diperoleh protease halostabil memiliki aktivitas spesifik tertinggi pada fraksi 1 (0-20%) sebesar 220,83 Unit/mg protein. Pengaruh garam NaCl, CaCl2 dan MgCl2 akan cenderung meningkatkan aktivitas proteasenya sebaliknya KCl akan menurunkan aktivitas protease. Profil pengaruh penambahan garam terhadap aktivitas protease semakin meningkat sesuai dengan urutan KCl. NaCl, CaCl2, MgCl2.

Keywords

bakteri halofilik; protease halostabil; bittern; garam monovalen; divalen

  1. Siti Anisa 
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  2. Nies Suci Mulyani 
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  3. Mukhammad Asy’ari  Orcid Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
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