Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA16754, author = {Rahmad Budiharjo and Purbowatiningrum Sarjono and Mukhammad Asy’ari}, title = {Pengaruh Konsentrasi NaCl Terhadap Aktivitas Spesifik Protease Ekstraseluler dan Pertumbuhan Bakteri Halofilik Isolat Bittern Tambak Garam Madura}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {20}, number = {3}, year = {2017}, keywords = {bakteri halofilik,; protease; azokasein; NaCl; aktivitas spesifik}, abstract = {Protease halofilik dapat dimanfaatkan pada proses fermentasi makanan seperti pada pembuatan kecap ikan. Penelitian ini bertujuan untuk memperoleh bakteri halofilik dari isolat bittern tambak garam Madura dan mengisolasi protease halofilik ekstraseluler serta menentukan pengaruh konsentrasi NaCl terhadap aktivitas spesifik protease halofilik. Bakteri halofilik ditumbuhkan pada media HSB ( Halophlie Synthetic Broth ). Penentuan aktivitas protease dilakukan dengan menggunakan substrat azokasein dan kadar protein ditentukan dengan menggunakan metode Lowry. Berdasarkan penelitian diperoleh bakteri halofilik isolat bittern tambak garam Madura yang tumbuh optimal pada konsentrasi NaCl 4 %(b/v), dengan aktivitas spesifik protease halofilik ekstraseluler tertinggi pada fraksi 4 (60-80 %) sebesar 58,537 Unit/mg protein. Adanya penambahan garam NaCl dapat meningkatkan aktivitas protease halofilik. Pada penelitian ini, aktivitas spesifik protease halofilik meningkat menjadi 113,78 Unit/mg protein dengan konsentrasi optimal NaCl 0,750 M}, issn = {2597-9914}, pages = {142--145} doi = {10.14710/jksa.20.3.142-145}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/16754} }
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