skip to main content

Pengaruh Konsentrasi NaCl Terhadap Aktivitas Spesifik Protease Ekstraseluler dan Pertumbuhan Bakteri Halofilik Isolat Bittern Tambak Garam Madura

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Oct 2017.
Open Access Copyright 2017 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0/.

Citation Format:
Abstract
Protease halofilik dapat dimanfaatkan pada proses fermentasi makanan seperti pada pembuatan kecap ikan. Penelitian ini bertujuan untuk memperoleh bakteri halofilik dari isolat bittern tambak garam Madura dan mengisolasi protease halofilik ekstraseluler serta menentukan pengaruh konsentrasi NaCl terhadap aktivitas spesifik protease halofilik. Bakteri halofilik ditumbuhkan pada media HSB (Halophlie Synthetic Broth). Penentuan aktivitas protease dilakukan dengan menggunakan substrat azokasein dan kadar protein ditentukan dengan menggunakan metode Lowry. Berdasarkan penelitian diperoleh bakteri halofilik isolat bittern tambak garam Madura yang tumbuh optimal pada konsentrasi NaCl 4 %(b/v), dengan aktivitas spesifik protease halofilik ekstraseluler tertinggi pada fraksi 4 (60-80 %) sebesar 58,537 Unit/mg protein. Adanya penambahan garam NaCl dapat meningkatkan aktivitas protease halofilik. Pada penelitian ini, aktivitas spesifik protease halofilik meningkat menjadi 113,78 Unit/mg protein dengan konsentrasi optimal NaCl 0,750 M
Fulltext View|Download
Keywords: bakteri halofilik,; protease; azokasein; NaCl; aktivitas spesifik

Article Metrics:

  1. Aharon Oren, Halophilic Microorganisms and their Environments, Springer Science & Business Media, 2002
  2. Shiladitya DasSarma, Priya DasSarma, Halophiles, in: Encyclopedia of Life Science, Wiley, 2006
  3. Neil D. Rawlings, Protease Families, Evolution and Mechanism of Action, in: K. Brix, W. Stöcker (Eds.) Proteases: Structure and Function, Springer Vienna, Vienna, 2013, pp. 1-36
  4. Jesse Charney, Rudolph M. Tomarelli, A colorimetric method for the determination of the proteolytic activity of duodenal juice, Journal of Biological Chemistry, 171, 2, (1947) 501-505
  5. Clive Edwards, Microbiology of extreme environments, McGraw-Hill, 1990
  6. Janos K. Lanyi, Salt-dependent properties of proteins from extremely halophilic bacteria, Bacteriological Reviews, 38, 3, (1974) 272-290
  7. Janos K. Lanyi, Joann Stevenson, Studies of the Electron Transport Chain of Extremely Halophilic Bacteria: IV. Role of Hydrophobic Forces in The Structure of Menadione Reductase, Journal of Biological Chemistry, 245, 16, (1970) 4074-4080
  8. Malashetty Vidyasagar, S Prakash, Carol Litchfield, K Sreeramulu, Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101, Archaea, 2, 1, (2006) 51-57
  9. Werasit Kanlayakrit, Preeyanuch Bovornreungroj, Takuji Oka, Masatoshi Goto, Production and characterization of protease from an extremely halophilic Halobacterium sp. PB407, Kasetsart Journal: Natural Science, 38, 5, (2004) 15-20

Last update:

  1. Characterization of extracellular protease from halophilic microbes isolated from Bledug Kuwu, Grobogan, Central Java

    Nur Faridah, Suharti Suharti. INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020), 2353 , 2021. doi: 10.1063/5.0052644
  2. Exploration and characterization of proteases produced by halophilic microbes isolated from salt-concentrating pond at Pasuruan, East Java

    Nailar Rokhmah, Nur Faridah, Evi Susanti, Suharti Suharti. INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020), 2353 , 2021. doi: 10.1063/5.0052651
  3. Characterization of a Halostable Metalloprotease from the Halophilic Bacterium Bacillus clausii J1G-0%B

    Annisa Dila Febriyanti, Mukhammad Asy'ari. Jurnal Kimia Sains dan Aplikasi, 27 (8), 2024. doi: 10.14710/jksa.27.8.395-402

Last update: 2024-10-13 19:18:58

No citation recorded.