Antioxidant from Turmeric Fermentation Products (Curcuma longa) by Aspergillus Oryzae

DOI: https://doi.org/10.14710/jksa.21.1.13-18
License URL: http://creativecommons.org/licenses/by-sa/4.0/

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Article Info
Published: 31-01-2018
Section: Research Articles
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The antioxidant capacity of natural materials can be improved by fermentation. In this study, turmeric rhizome was fermented by Aspergillus oryzae. Fermentation products were obtained with fermentation time of 14, 21 and 28 hours. Furthermore, the fermentation product was extracted using ethanol and determined its antioxidant capacity by DPPH damping method, total phenolate determination and phytochemical screening. The results showed that the antioxidant capacity of fermentation products was higher than without fermentation. Antioxidant activity increased with length of fermentation with the value of antioxidant capacity without fermentation and with incubation for 14, 21, and 28 days respectively of 17.0; 27.3; 33.3; and 34.1 mg quercetin/gram extract. The total phenolate of fermentation products was 261; 324,3; 361; 374.3 mg of gallic acid/gram extract. Ethanol extracts of fermented and non-fermented products all contain entirely positive alkaloids, saponins, flavonoids, tannins, quinones, and steroids.

Keywords

turmeric; fermentation; Aspergillus oryzae; antioxidant; total phenolic; phytochemicals

  1. Sulasiyah Sulasiyah 
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  2. Purbowatiningrum Ria Sarjono  Orcid Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  3. Agustina L. N. Aminin  Orcid Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  1. M Karamac, R Amarowicz, Antioxidant activity of BHA, BHT and TBHQ examined with Miller's test, Grasas y aceites, 48, 2, (1997) 83-86 http://dx.doi.org/10.3989/gya.1997.v48.i2.772
  2. Buyung Rukmantara Susena Putra, Dewi Kusrini, Enny Fachriyah, Isolasi Senyawa Antioksidan dari Fraksi Etil Asetat Daun Tempuyung (Sonchus arvensis L), Jurnal Kimia Sains dan Aplikasi, 16, 3, (2013) 69-72
  3. Dita Widia Ningrum, Dewi Kusrini, Enny Fachriyah, Uji Aktivitas Antioksidan Senyawa Flavonoid dari Ekstrak Etanol Daun Johar (Senna siamea Lamk), Jurnal Kimia Sains dan Aplikasi, 20, 3, (2017) 123-129
  4. I Kuncahyono, Uji Aktivitas Antioksidan Ekstrak Belimbing Wuluh (Averrhoa bilimbi, l.) terhadap 1, 1-Diphenyl-2-Picrylhidrazyl (DPPH), D-III. Yogyakarta: Teknologi Farmasi Fakultas Teknik Universitas Setia Budi, (2007)
  5. Agustina Arpintasari, Wuryanti Wuryanti, Wasino Hadi Rahmanto, Isolasi dan Uji Potensi L-Asparaginase dari Rimpang Kunyit Putih (Curcuma mangga Vall) terhadap Leukimia Tipe K562, Jurnal Kimia Sains dan Aplikasi, 11, 3, (2008) 57-62
  6. Jeffrey Barry Harborne, Metode fitokimia, Padmawinata K, S. I, Institut Teknologi Bandung, Bandung, 1987.
  7. Philip Molyneux, The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin Journal of Science and Technology, 26, 2, (2004) 211-219
  8. E Berghofer, B Grzeskowiak, N Mundigler, WB Sentall, J Walcak, Antioxidative properties of faba bean-, soybean-and oat tempeh, International Journal of Food Sciences and Nutrition, 49, 1, (1998) 45-54
  9. Linghong Liang, Xiangyang Wu, Ting Zhao, Jiangli Zhao, Fang Li, Ye Zou, Guanghua Mao, Liuqing Yang, In vitro bioaccessibility and antioxidant activity of anthocyanins from mulberry (Morus atropurpurea Roxb.) following simulated gastro-intestinal digestion, Food Research International, 46, 1, (2012) 76-82 http://dx.doi.org/10.1016/j.foodres.2011.11.024
  10. AA Prabhu, CM Mrudula, J Rajesh, Effect of yeast fermentation on nutraceutical and antioxidant properties of rice bran, Int J Agric Food Sci, 4, (2014) 59-65
  11. MS Brewer, Natural antioxidants: sources, compounds, mechanisms of action, and potential applications, Comprehensive reviews in food science and food safety, 10, 4, (2011) 221-247
  12. Dejian Huang, Boxin Ou, Ronald L Prior, The chemistry behind antioxidant capacity assays, Journal of agricultural and food chemistry, 53, 6, (2005) 1841-1856 http://dx.doi.org/10.1021/jf030723c
  13. V. Bondet, W. Brand-Williams, C. Berset, Kinetics and Mechanisms of Antioxidant Activity using the DPPH.Free Radical Method, LWT - Food Science and Technology, 30, 6, (1997) 609-615 http://dx.doi.org/10.1006/fstl.1997.0240