1Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
2Otolaryngology Department, Faculty of Medicine, Diponegoro University, Indonesia
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@article{JKSA17430, author = {Putri Wardani and Suprihati Suprihati and Wuryanti Wuryanti}, title = {Uji Antikanker Isolat Bioaktif L-asparaginase dari Kunyit (Curcuma domestica Val) terhadap Sel Kanker Serviks}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {14}, number = {3}, year = {2011}, keywords = {Kanker serviks; L-asparaginase; antikanker; kunyit; nilai LC50}, abstract = {Kanker serviks merupakan penyebab kematian terbesar pada wanita di negara berkembang. Berbagai metode terapi penyakit ini telah dilakukan, di antaranya dengan penggunaan senyawa bioaktif sebagai agen antikanker, salah satunya adalah kunyit ( Curcuma domestica Val). Penelitian ini bertujuan untuk mengisolasi, memurnikan, menentukan nilai aktivitas spesifik tertinggi, nilai LC 50 dan potensi antikanker enzim L- asparaginase dari kunyit pada kultur sel kanker serviks (HeLa). Enzim L- asparaginase diisolasi dengan metode ekstraksi kemudian dimurnikan dengan fraksinasi dan dialisis. Uji aktivitas L- asparaginase menggunakan metode Nessler, sedangkan penentuan kadar protein dilakukan dengan metode Lowry. Aktivitas spesifik ditentukan melalui perbandingan unit aktivitas terhadap kadar protein. Fraksi enzim L- asparaginase yang memiliki aktivitas spesifik tertinggi diuji sitotoksisitasnya terhadap sel HeLa dengan metode MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazoliumbromida) dan potensi antikankernya ditentukan dengan menghitung nilai LC 50 . Hasil penelitian disimpulkan bahwa enzim L- asparaginase dapat diisolasi dan dimurnikan dari kunyit dengan aktivitas spesifik tertinggi pada fraksi 4 sebesar 284,040 unit/mg protein. Uji sitotoksisitas pada sel HeLa menunjukkan nilai LC 50 sebesar 91,833 μg/mL, sehingga isolat enzim L- asparaginase fraksi 4 dari kunyit mampu menghambat pertumbuhan sel HeLa, tetapi kurang berpotensi sebagai agen antikanker berdasarkan standar NCI (National Cancer Institute).}, issn = {2597-9914}, pages = {89--93} doi = {10.14710/jksa.14.3.89-93}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/17430} }
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