Isolasi dan Karakterisasi Kitinase Isolat Jamur Akuatik Kitinolitik KC3 dari Kecoa (Orthoptera)

Karso Karso -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
*Wuryanti Wuryanti -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
Sriatun Sriatun -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
Published: 1 Aug 2014.
Open Access Copyright 2014 Jurnal Kimia Sains dan Aplikasi

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Abstract
Isolasi dan karakterisasi kitinase dari isolat jamur akuatik kitinolitik KC3 hasil isolasi bangkai kecoa telah dilakukan. Penelitian ini bertujuan untuk mendapatkan kitinase dari isolat jamur akuatik KC3 dan mendapatkan informasi karakter kitinase hasil isolasi yang meliputi pH optimum dan suhu optimum. Kitinase adalah enzim kompleks terdiri dari endokitinase, kitobiosidase dan N-asetilglukosaminidase. Media produksi mengandung kitin koloidal sebagai inducer. Fraksinasi dilakukan dengan amonium sulfat sampai tingkat kejenuhan 90% (F5). Aktivitas kitinase diukur dengan metode Ueda-Arai berdasarkan pengurangan substrat. Aktivitas spesifik paling tinggi diperoleh pada fraksi satu (F1) yaitu sebesar 73,258 U/mg. Hasil karakterisasi kitinase diperoleh profil pH optimum kitinase pada pH 3,8 sedangkan profil suhunya diperoleh dua puncak yaitu pada 28,5°C dan 29,5°C. Profil suhu dua puncak/titik menunjukkan jika kemungkinan terdapat dua jenis kitinase pada enzim yang diuji.
Keywords
kitinase; N-asetilglukosamin; jamur akuatik KC3; kecoa

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