Improvement of Bioactivity with Nanoparticle Fabrication: Cytotoxic Test of Ethanol, N-Hexane and Ethyl Acetate Extract from Red Galangal Rhizome (Alpinia purpurata (Vieill.) K. Schum) in Bulk and Nanoparticle size using BSLT Method

DOI: https://doi.org/10.14710/jksa.21.1.39-43

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Published: 31-01-2018
Section: Research Articles
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Some of the secondary metabolites present in red algae are terpenoids, quinones, flavonoids, alkaloids, essential oils, diarylheptanoids, steroids, cardioglycosides, oils and fats, tannins, carbohydrates. Activity of rhizomes, leaves and flowers red leaf is as antimicrobial, anti-fungal, anti-oxidants, anti-tumor, anti-cancer and vasodilator. One way to improve the physical, chemical and bioactivity properties of natural compounds was to make them into nanoparticles. In this study, the isolation of bioactive compounds contained in red laos rhizome by maceration method using ethanol solvent was done, then partitioned with n-hexane and ethyl acetate. The extracts thus obtained are fabricated into nanoparticles. Extracts in bulk and nanoparticles were then tested for cytotoxic activity using BSLT method. Results of analysis with PSA showed that ethanol extract had size 410,8 nm, n-hexane extract 220,7 nm and ethyl acetate extract 208,3 nm. The results of cytotoxic tests showed that nanoparticle size increased cytotoxic activity. Ethyl acetate extract was most active compared to ethanol and n-hexane extracts with LC50values of 17.919; 84,956; 166,526 ppm. Whereas the nanoparticle size was respectively 10,491; 74,072 and 84,197 ppm. Cytotoxic activity increases with nanoparticle fabrication.

 

Keywords

Red galangal; nanoparticles; maceration; ethanol; n-hexane; ethyl acetate; cytotoxic activity; BSLT; LC50

  1. Enny Fachriyah  Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  2. Dewi Kusrini  Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  3. Pratama Jujur Wibawa  Orcid Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  1. Ayu Ni’mah Azifa, Dewi Kusrini, Enny Fachriyah, Identifikasi Senyawa Sitotoksik dalam Ekstrak Kloroform Daun Tempuyung (Sonchus arvensis L.) Menggunakan GC-MS, Jurnal Kimia Sains dan Aplikasi, 17, 1, (2014) 23-26
  2. Ika Pratiwi Khosimah Adinata, Khairul Anam, Dewi Kusrini, Identifikasi Senyawa Metabolit Sekunder Fraksi Aktif Daun Jarak Pagar (Jatropha curcas L.) dan Uji Aktivitas Larvasida terhadap Larva Nyamuk Aedes aegypti, Jurnal Kimia Sains dan Aplikasi, 16, 2, (2013) 42-45
  3. PN Ravindran, I Balachandran, Under utilized medicinal spices, Spice India, 17, 12, (2004) 2-14
  4. Fajar Budi Laksono, Enny Fachriyah, Dewi Kusrini, Isolasi dan Uji Antibakteri Senyawa Terpenoid Ekstrak N-Heksana Rimpang Lengkuas Merah (Alpinia purpurata), Jurnal Kimia Sains dan Aplikasi, 17, 2, (2014) 37-42
  5. Yuharmen, Yum Eryanti, Nurbalatif, Uji Aktivitas Antimikroba Minyak Atsiri dan Ekstrak Metanol Lengkuas (Alpinia galanga), FMIPA, Universitas Riau, Riau
  6. Chinthamony Arul Raj, Paramasivam Ragavendran, Dominic Sophia, Muthaiyan Ahalliya Rathi, Velliyur Kanniappan Gopalakrishnan, Evaluation of in vitro antioxidant and anticancer activity of Alpinia purpurata, Chinese Journal of Natural Medicines, 10, 4, (2012) 263-268 http://dx.doi.org/10.1016/S1875-5364(12)60053-3
  7. CP Victório, RM Kuster, CLS Lage, Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography, Revista Brasileira de Plantas Medicinais, 11, 2, (2009) 147-153
  8. Ratna Susilaningsih, Isolasi, Identifikasi dan Uji Toksisitas Senyawa Alkaloid Fraksi Etil Asetat Rimpang Lengkuas Merah (Alpinia galanga), Kimia, Universitas Diponegoro, Semarang
  9. Enock Kiage Oirere, Palanirajan Anusooriya, Chinthamony Arul Raj, Velliyur Kanniappan Gopalakrishnan, Phytochemical Analysis of N-Hexane Leaf Extract of Alpinia purpurata (Vieill.) K. Schum Using Uv-Vis, FTIR and GC-MS, International Journal of Pharmacy and Pharmaceutical Sciences 7, 8, (2015) 387-389
  10. Yuharmen, Yum Eryanti, Nurbalatif, Uji Aktivitas Antimikroba Minyak Atsiri dan Ekstrak Metanol Lengkuas (Alpinia galanga), in, Jurusan Kimia, FMIPA, Universitas Riau, 2002.
  11. KP Kochuthressia, S John Britto, MO Jaseentha, L Joelri Michael Raj, SR Senthilkumar, Antimicrobial efficacy of extracts from Alpinia purpurata (Vieill.) K. Schum. against human pathogenic bacteria and fungi, Agriculture and Biology Journal of North America, 1, 6, (2010) 1249-1252 http://dx.doi.org/10.5251/abjna.2010.1.6.1249.1252
  12. T. Juntachote, E. Berghofer, Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal, Food Chemistry, 92, 2, (2005) 193-202 http://dx.doi.org/10.1016/j.foodchem.2004.04.044
  13. Hideji Itokawa, Koichi Takeya, Antitumor substances from higher plants, ChemInform, 24, 52, (1993)
  14. Cristiane Pimentel Victório, Ricardo Machado Kuster, Roberto Soares de Moura, Celso Luiz Salgueiro Lage, Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro, Brazilian Journal of Pharmaceutical Sciences, 45, 3, (2009) 507-514
  15. R. Kelmani Chandrakanth, C. Ashajyothi, A. K. Oli, C. Prabhurajeshwar, Potential Bactericidal Effect of Silver Nanoparticles Synthesised from Enterococcus Species, Oriental Journal of Chemistry, 30, 3, (2014) 1253-1262 http://dx.doi.org/10.13005/ojc/300341
  16. Rainer H. Müller, Sven Gohla, Cornelia M. Keck, State of the art of nanocrystals – Special features, production, nanotoxicology aspects and intracellular delivery, European Journal of Pharmaceutics and Biopharmaceutics, 78, 1, (2011) 1-9 http://dx.doi.org/10.1016/j.ejpb.2011.01.007
  17. Matthew N. Rhyner, Andrew M. Smith, Xiaohu Gao, Hui Mao, Lily Yang, Shuming Nie Nie, Quantum dots and multifunctional nanoparticles: new contrast agents for tumor imaging, Nanomedicine, 1, 2, (2006) 209-217 http://dx.doi.org/10.2217/17435889.1.2.209
  18. Huanhuan Li, Quansheng Chen, Jiewen Zhao, Khulal Urmila, Enhancing the antimicrobial activity of natural extraction using the synthetic ultrasmall metal nanoparticles, Scientific reports, 5, (2015) 11033 http://dx.doi.org/10.1038/srep11033
  19. Rupesh Kumar Basniwal, Harpreet Singh Buttar, V. K. Jain, Nidhi Jain, Curcumin Nanoparticles: Preparation, Characterization, and Antimicrobial Study, Journal of Agricultural and Food Chemistry, 59, 5, (2011) 2056-2061 http://dx.doi.org/10.1021/jf104402t
  20. B. N. Meyer, N. R. Ferrigni, J. E. Putnam, L. B. Jacobsen, D. E. Nichols, J. L. McLaughlin, Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents, Planta Med, 45, 05, (1982) 31-34 http://dx.doi.org/10.1055/s-2007-971236
  21. Inbar Yariv, Anat Lipovsky, Aharon Gedanken, Rachel Lubart, Dror Fixler, Enhanced pharmacological activity of vitamin B12 and penicillin as nanoparticles, International journal of nanomedicine, 10, (2015) 3593
  22. Stephanie M Reimann, Matti Manninen, Electronic structure of quantum dots, Reviews of Modern Physics, 74, 4, (2002) 1283