1Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
2Department of Chemistry, Bandung Institute of Technology, Indonesia
3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Indonesia
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@article{JKSA18885, author = {Agustina Aminin and Akhmaloka Akhmaloka and Hendro Pramono}, title = {DNA Polimerase sebagai Model Kajian Biomolekul Enzim Termostabil Isolat Lokal}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {3}, number = {2}, year = {2000}, keywords = {Ekstremofil; enzim termostabil; DNA polimerase; kloning; pustaka genom}, abstract = {Ekstremozim merupakan bahan kajian penelitian yang banyak dilakukan dalam dekade terakhir. Enzim ini merupakan biokatalis yang sangat efektif digunakan dalam proses industri. Salah satu sumber ekstremozim yang cukup potensial adalah bakteri termofilik, tumbuh pada sumber air panas. Indonesia merupakan negara yang kaya akan sumber air panas, tetapi sampai saat ini belum banyak peneliti yang mengeksplorasi bakteri termofilik. Salah satu bakteri termofilik dari Cimanggu, suatu sumber air panas di sekitar Bandung yang memiliki suhu sekitar 80°C telah berhasil diisolasi. DNA polimerase dipilih sebagai enzim model untuk mempelajari struktur fungsi dari enzim termostabil. Kloning terhadap gen pengkode DNA polimerase dilakukan dengan pendekatan pustaka genom dan amplifikasi genom menggunakan teknik PCR. Primer dirancang berdasarkan homologi urutan nukleotida gen pengkode DNA polimerase dari Thermus aquaticus dan Thermus thermophilus. Primer P1-P3 mengamplifikasi empat fragmen berukuran 1 kb, 1,4 kb, 1,6 kb dan 1,8 kb yang tidak saling overlap. Dalam hal ini fragmen DNA 1 kb telah ditentukan urutan nukleotidanya.}, issn = {2597-9914}, pages = {79--84} doi = {10.14710/jksa.3.2.79-84}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/18885} }
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