Antiglycation and Antioxidant Activity from Methanol Extract and Fraction of Xylocarpus granatum Stem

Intan Noviarni  -  Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Indonesia
*Irmanida Batubara orcid scopus  -  Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Indonesia
Sastia Prama Putri orcid scopus  -  Biotechnology Study Program, Faculty of Engineering, Osaka University, Japan
Received: 4 Oct 2019; Revised: 30 Dec 2019; Accepted: 31 Dec 2019; Published: 31 Jan 2020; Available online: 31 Jan 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0

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Abstract
The objective of this research was to determine the antioxidant and antiglycation activity of methanol crude extract of Xylocarpus granatum stem and its fraction. The methanol crude extract was fractioned by liquid-liquid extraction to obtain three fractions, which were n-hexane fraction, ethyl acetate fraction, and methanol fraction. Antioxidant activity was performed using scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antiaging activity was identified based on the ability to inhibit the formation of advanced glycation end products (AGEs). The results of the study showed that methanol crude extract and all fractions had an antioxidant and antiglycation activity that significantly different from each other (P<0.05). The results show that the most active antiglycation and antioxidant activity was the methanol fraction with IC50 were 71.55 ppm and 8.52 ppm, respectively. The methanol fraction contained an alkaloid, flavonoid, phenolic, and triterpenoid compounds. Subsequent fractionation of the methanol fraction guided by bioassay showed that sub-fraction 1 had the best antiglycation and antioxidant activity. Based on the color of the chromatogram obtained at UV 366 nm, the chemical component contained in the most active fraction was the flavonoid group.
Keywords: advanced glycation end products; antioxidants; DPPH; Xylocarpus granatum
Funding: Ministry of Research Technology and Higher Education of Republic of Indonesia

Article Metrics:

  1. Wendy del Dzib-Guerra, Fabiola Escalante-Erosa, Karlina Garcia-Sosa, Severine Derbre, Patricia Blanchard, Pascal Richomme and Luis Pena-Rodriguez, Anti-Advanced glycation end-product and free radical scavenging activity of plants from the yucatecan flora, Pharmacognosy Research, 8, 4, (2016) 276-280 https://doi.org/10.4103/0974-8490.188883
  2. Gugulethu Ndlovu, Gerda Fouche, Malefa Tselanyane, Werner Cordier and Vanessa Steenkamp, In vitro determination of the anti-aging potential of four southern African medicinal plants, BMC Complementary and Alternative Medicine, 13, 1, (2013) 304 https://doi.org/10.1186/1472-6882-13-304
  3. Izabela Grzegorczyk-Karolak, Krzysztof Gołąb, Jakub Gburek, Halina Wysokińska and Adam Matkowski, Inhibition of Advanced Glycation End-Product Formation and Antioxidant Activity by Extracts and Polyphenols from Scutellaria alpina L. and S. altissima L, Molecules, 21, 6, (2016) https://doi.org/10.3390/molecules21060739
  4. Christiane Ott, Kathleen Jacobs, Elisa Haucke, Anne Navarrete Santos, Tilman Grune and Andreas Simm, Role of advanced glycation end products in cellular signaling, Redox Biology, 2, (2014) 411-429 https://doi.org/10.1016/j.redox.2013.12.016
  5. Chan-Sik Kim, Sok Park and Junghyun Kim, The role of glycation in the pathogenesis of aging and its prevention through herbal products and physical exercise, Journal of Exercise Nutrition & Biochemistry, 21, 3, (2017) 55-61 https://doi.org/10.20463/jenb.2017.0027
  6. Mami Yokota, Hitoshi Masaki, Yuri Okano and Yoshihiro Tokudome, Effect of glycation focusing on the process of epidermal lipid synthesis in a reconstructed skin model and membrane fluidity of stratum corneum lipids, Dermato-Endocrinology, 9, 1, (2017) e1338992 https://doi.org/10.1080/19381980.2017.1338992
  7. Sakina Yagi, Natalia Drouart, Frédéric Bourgaud, Max Henry, Yves Chapleur and Dominique Laurain-Mattar, Antioxidant and antiglycation properties of Hydnora johannis roots, South African Journal of Botany, 84, (2013) 124-127 https://doi.org/10.1016/j.sajb.2012.10.006
  8. Ashok Shinde, Jayashree Ganu and Pankaja Naik, Effect of free radicals & antioxidants on oxidative stress: a review, Journal of Dental and Allied Sciences, 1, 2, (2012) 63-66 https://doi.org/10.4103/2277-4696.159144
  9. Mohamad Gazali, Neviaty P Zamani and Irmanida Batubara, Potensi limbah kulit buah nyirih Xylocarpus granatum sebagai inhibitor tirosinase, DEPIK Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan, 3, 3, (2014) 187-194 https://doi.org/10.13170/depik.3.3.5711
  10. I Batubara, LK Darusman, T Mitsunaga, M Rahminiwati and E Djauhari, Potency of Indonesian medicinal plants as tyrosinase inhibitor and antioxidant agent, Journal of Biological Sciences, 10, 2, (2010) 138-144 http://dx.doi.org/10.3923/jbs.2010.138.144
  11. K. Darusman Latifah, Batubara Irmanida and Lopolisa Catharina, Screening Marker Components of Tyrosinase Inhibitor From Xylocarpus Granatum Stem, Jurnal Kimia Valensi, 2, 3, (2011) 409-413 https://doi.org/10.15408/jkv.v2i3.111
  12. Udom Kokpol, Warinthorn Chavasiri, Santi Tip-pyang, Gaysorn Veerachato, Fenglin Zhao, Jim Simpson and Rex T. Weavers, A limonoid from Xylocarpus granatum, Phytochemistry, 41, 3, (1996) 903-905 https://doi.org/10.1016/0031-9422(95)00724-5
  13. Alison F. Stallings and Mary P. Lupo, Practical Uses of Botanicals in Skin Care, J Clin Aesthet Dermatol, 2, 1, (2009) 36-40
  14. Jeffrey B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Springer Netherlands, 2013
  15. Adawiah Adawiah, Kandungan Fitokimia dan Bioaktivitas Ekstrak Metanol Biji Palem Putri (Veitchia merillii), Jurnal Kimia Valensi, 2, 1, (2016) 63-70 http://dx.doi.org/10.15408/jkv.v2i1.3076
  16. Nasapon Povichit, Ampai Phrutivorapongkul, Maitree Suttajit and Pimporn Leelapornpisid, Antiglycation and antioxidant activities of oxyresveratrol extracted from the heartwood of Artocarpus lakoocha Roxb, Maejo International Journal of Science and Technology, 4, 3, (2010) 454-461
  17. Deni Ramdani, Marjuki Majuki and Siti Chuzaemi, Pengaruh perbedaan jenis pelarut dalam proses ekstraksi buah mengkudu (Morinda citrifolia L.) pada pakan terhadap viabilitas protozoa dan produksi gas in-vitro, Jurnal Ilmu-Ilmu Peternakan, 27, 2, (2017) 54-62 https://doi.org/10.21776/ub.jiip.2017.027.02.07
  18. Buyung Rukmantara Susena Putra, Dewi Kusrini and Enny Fachriyah, Isolasi Senyawa Antioksidan dari Fraksi Etil Asetat Daun Tempuyung (Sonchus arvensis L), Jurnal Kimia Sains dan Aplikasi, 16, 3, (2013) 69-70 https://doi.org/10.14710/jksa.16.3.69-72
  19. Irmanida Batubara, Ummi Zahra, Latifah K. Darusman and Akhirudin Maddu, Minyak atsiri daun zingiberaceae sebagai antioksidan dan antiglikasi, Indonesian Journal of Essential Oil, 1, 1, (2016) 44-52 http://dx.doi.org/10.21776/ub.ijeo.2016.001.01.05
  20. Agnieszka Piwowar, Anna Rorbach-Dolata and Izabela Fecka, The Antiglycoxidative Ability of Selected Phenolic Compounds—An In Vitro Study, Molecules, 24, 15, (2019) https://doi.org/10.3390/molecules24152689
  21. Shaikh J. Uddin, Lutfun Nahar, Jamil A. Shilpi, Mohammad Shoeb, Tomasz Borkowski, Simon Gibbons, Moira Middleton, Maureen Byres and Satyajit D. Sarker, Gedunin, a limonoid from Xylocarpus granatum, inhibits the growth of CaCo-2 colon cancer cell line In Vitro, Phytotherapy Research, 21, 8, (2007) 757-761 https://doi.org/10.1002/ptr.2159
  22. Musa Chacha, Flavanol derivatives with antioxidant activity from the stem bark of Xylocarpus granatum, International Journal of Biological and Chemical Sciences, 4, 2, (2010) 371-376 http://dx.doi.org/10.4314/ijbcs.v4i2.58127
  23. Shijie Du, Mingan Wang, Wen Zhu and Zhaohai Qin, A new fungicidal lactone from Xylocarpus granatum (Meliaceae), Natural Product Research, 23, 14, (2009) 1316-1321 https://doi.org/10.1080/14786410902726183
  24. Sweta Misra, Meenakshi Verma, Sunil Kumar Mishra, Shishir Srivastava, Vijai Lakshmi and Shailja Misra-Bhattacharya, Gedunin and photogedunin of Xylocarpus granatum possess antifilarial activity against human lymphatic filarial parasite Brugia malayi in experimental rodent host, Parasitology Research, 109, 5, (2011) 1351 https://doi.org/10.1007/s00436-011-2380-x
  25. Qiang Gao, Yuan Gao, Han Song, Jianli Li, Yibing Wu, Xiaowei Shi, Haishui Shi and Yuxia Ma, Cipadesin A, a bioactive ingredient of Xylocarpus granatum, produces antidepressant-like effects in adult mice, Neuroscience Letters, 633, (2016) 33-39 https://doi.org/10.1016/j.neulet.2016.08.062
  26. Xi Yin, Xin Li, Yaoguang Hao, Yiwen Zhao, Jinghong Zhou and Haishui Shi, Xylocarpin H, a Limonoid of Xylocarpus granatum, Produces Antidepressant-Like Activities in Mice, Journal of Behavioral and Brain Science, 5, 11, (2015) 524-532 https://doi.org/10.4236/jbbs.2015.511050
  27. Yi-Guo Dai, Jun Wu, Krishna Pillai Padmakumar and Li Shen, Sundarbanxylogranins A–E, five new limonoids from the Sundarban Mangrove, Xylocarpus granatum, Fitoterapia, 122, (2017) 85-89 https://doi.org/10.1016/j.fitote.2017.08.013
  28. Haroon Ahmad, Ibrar Khan and Wagma Nisar, Antioxidation and Antiglycation Properties of Bunium Bulbocastanum Fruits Various Fractions and its Possible Role in Reducing Diabetes Complication and Ageing, Vitamins & Minerals, 3, 1, (2014) 1000118 https://doi.org/10.4172/2376-1318.1000118
  29. Ummi Zahra, Yuni Kartika, Irmanida Batubara, Latifah K Darusman and Akhiruddin Maddu, Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent, Nusantara Bioscience, 8, 2, (2016) 221-225 https://doi.org/10.13057/nusbiosci/n080214
  30. Luc Séro, Lionel Sanguinet, Patricia Blanchard, T. Bach Dang, Sylvie Morel, Pascal Richomme, Denis Séraphin and Séverine Derbré, Tuning a 96-Well Microtiter Plate Fluorescence-Based Assay to Identify AGE Inhibitors in Crude Plant Extracts, Molecules, 18, 11, (2013) https://doi.org/10.3390/molecules181114320
  31. Philip Molyneux, The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin Journal of Science and Technology (SJST), 26, 2, (2004) 211-219
  32. Dita Widia Ningrum, Dewi Kusrini and Enny Fachriyah, Uji Aktivitas Antioksidan Senyawa Flavonoid dari Ekstrak Etanol Daun Johar (Senna siamea Lamk), Jurnal Kimia Sains dan Aplikasi, 20, 3, (2017) 123-129 https://doi.org/10.14710/jksa.20.3.123-129
  33. Haslina Haslina and Murtiari Eva, Extract Corn Silk with Variation of Solvents on Yield, Total Phenolics, Total Flavonoids and Antioxidant Activity, Indonesian Food and Nutrition Progress, 14, 1, (2017) 21-28 https://doi.org/10.22146/ifnp.24280
  34. Askal Maimulyanti and Anton Restu Prihadi, Chemical composition of essential oil and hexane extract and antioxidant activity of various extracts of Acmella uliginosa (Sw.) Cass flowers from Indonesia, Agriculture and Natural Resources, 50, 4, (2016) 264-269 https://doi.org/10.1016/j.anres.2015.11.002
  35. Do Hoang Thu Trang, Hoang Le Son and Phung Van Trung, Investigation on the in vitro antioxidant capacity of methanol extract, fractions and flavones from Oroxylum indicum Linn bark, Brazilian Journal of Pharmaceutical Sciences, 54, 1, (2018) 1-7 http://dx.doi.org/10.1590/s2175-97902018000117178
  36. K.R. Markham, Techniques of flavonoid identification, Academic Press, 1982
  37. Swagat Kumar Das, Dibyajyoti Samantaray, Jayanta Kumar Patra, Luna Samanta and Hrudayanath Thatoi, Antidiabetic potential of mangrove plants: a review, Frontiers in Life Science, 9, 1, (2016) 75-88 https://doi.org/10.1080/21553769.2015.1091386

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