Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain

Teuku Mohamad Iqbalsyah; Muhammad A Velayati; Hira Helwati; Febriani Febriani

doi:10.14710/jksa.23.5.183-188

DOI: https://doi.org/10.14710/jksa.23.5.183-188

Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain

Teuku Mohamad Iqbalsyah

, Muhammad A Velayati, Hira Helwati

, Febriani Febriani

Biomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Indonesia

Received: 24 Feb 2020; Revised: 7 Apr 2020; Accepted: 30 Apr 2020; Published: 31 May 2020.

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Abstract

The search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method. A thermo-halophilic bacterium has been isolated from an undersea hot spring, dubbed Pria Laot Sabang (PLS) A strain. The 0.9 kb DNA Polymerase I gene fragments from the isolate were amplified, sequenced, and identified. The fragments were part of the polymerization domain of the enzyme. Homological analysis of the gene sequence showed that the PLS A strain was closely related to Bacillus caldolyticusstrain XM. However, Swissprot structural analysis reveals that PLS A strain had high homology to Geobacillus stearathermophilus. Full sequence analysis is still needed to identify the species and evaluate the intact enzyme structure.

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Keywords: DNA polymerase; Geobacillus; halophilic; thermophilic; Sabang

Funding: Universitas Syiah Kuala

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Section: 8th - International Conference of the Indonesian Chemical Society (ICICS)

Language : EN

In Vol 23, No 5 (2020): Volume 23 Issue 5 Year 2020

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Last update: 2026-09-05 03:45:11

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