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Validation of High-Performance Liquid Chromatography Method for Determination of Vitamin B1 in Powder Milk

Department of Chemical Analysis, Politeknik AKA Bogor, Indonesia

Received: 8 Dec 2019; Revised: 6 Apr 2020; Accepted: 20 Apr 2020; Published: 31 May 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
Vitamin B1 plays an important role in the co-enzymatic reactions for energy-rich compounds called ATP (Adenosine Tri Phosphate). Therefore, it should be added to various food products, for example, milk powder. One method that can be used to determine vitamin B1 is SNI number 3751: 2009, but the method is intended for wheat flour. If the method is to be used for the analysis from other samples, such as milk powder, optimization, and validation, are needed. This experiment was carried out using HPLC, C18 column, and UV detector with a wavelength of 254 nm. The mobile phase used is methanol: acetic acid: bi-distilled water = 32:1:67 (v/v/v), flow rate = 1 mL/minute, isocratic, and reverse phased technique. Method validation parameters include tests of system suitability, linearity, the limit of detection, the limit of quantitation, precision (repeatability), and accuracy. The results showed that the system suitability test was obtained relative standard deviations (% RSD) for retention time and peak area, tailing factor, resolution, separation factor was 0.297%, 1.476%, 1.113, 6.693, and 4.406 respectively. The validation test gets a correlation coefficient (R) of 0.9996, the limit of detection and limit of quantitation were 0.0122 mg/100 mL and 0.0244 mg/100 mL, respectively. The precision test obtained Horwitz's ratio of 0.27%. Accuracy test using CRM obtained % recovery of 93.79-97.77%. All these results meet the requirements of method validation, so it can be concluded that the method of SNI number 3751: 2009 is valid for the determination of vitamin B1 in milk powder and can be used for routine analysis procedure.
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Keywords: HPLC validation; chromatography; vitamin B1; milk powder
Funding: Politeknik AKA Bogor

Article Metrics:

  1. Badan Standardisasi Nasional, Susu Bubuk, in, Badan Standardisasi Nasional, Jakarta, 2015
  2. William Alexander Newman Dorland and A.A. Mahode, Kamus saku kedokteran Dorland, 31st ed., Elsevier (Singapore), 2012
  3. F.G. Winarno, Kimia Pangan dan gizi, P.T. Gramedia, 1984
  4. L. Gámiz-Gracia and M. D. Luque de Castro, An HPLC Method for the Determination of Vitamin B1, Caffeine, Acetylsalicylic Acid, and The Impurities of Salicylic Acid in a Pharmaceutical Preparation, Journal of Liquid Chromatography & Related Technologies, 20, 13, (1997), 2123-2133 https://doi.org/10.1080/10826079708005570
  5. Amidzic Rada, Brboric Jasmina, Cudina Olivera and Vladimirov Sote, RP-HPLC Determination of vitamins B1, B3, B6, folic acid and B12 in multivitamin tablets, Journal of the Serbian Chemical Society, 70, 10, (2005), 1229–1235
  6. Novi Yantih, Diah Widowati, Wartini and Tiwi Aryani, Validation of HPLC method for determination of thiamine hydrochloride, riboflavin, nicotinamide, and pyridoxine hydrochloride in syrup preparation, Canadian Journal on Scientific and Industrial Research, 2, 7, (2011), 269-278
  7. Ahmet Haşİmoğlu and Shantanu B Ghodke, A novel RP-HPLC method for simultaneous determination of vitamins B1, B2, B3, B6 and C in oral powder for veterinary consumption, Marmara Pharmaceutical Journal, 22, 4, (2018), 484-492 https://doi.org/10.12991/jrp.2018.89
  8. Eurachem, The Fitness for Purpose of Analytical Methods: A Laboratory Guide to Method Validation and Related Topics, Eurachem, 2014
  9. AOAC, AOAC Guidelines for Single Laboratory; Validation of Chemical Methods for Dietary Supplements and Botanicals, in, 2002,
  10. Yandi Syukri, Ronny Martien, Endang Lukitaningsih and Agung Endro Nugroho, Quantification of Andrographolide Isolated from Andrographis paniculata Nees Obtained from Traditional Market in Yogyakarta Using Validated HPLC, Indonesian Journal of Chemistry, 16, 2, (2016), 190-197 https://doi.org/10.22146/ijc.21163
  11. Bhaskar Kurangi, Sunil Jalalpure and Satveer Jagwani, A validated stability-indicating HPLC method for simultaneous estimation of resveratrol and piperine in cubosome and human plasma, Journal of Chromatography B, 1122-1123, (2019), 39-48 https://doi.org/10.1016/j.jchromb.2019.05.017
  12. Retno Puji Lestari, Resi Gifrianto and Anwar Hadi, Penentuan Limit Deteksi Pengujian Gas Hidrogen Klorida (HCl) dari Emisi Sumber Tidak Bergerak Menggunakan Kromatografi Ion, Ecolab, 11, 1, (2017), 32-41 https://doi.org/10.20886/jklh.2017.11.1.32-41
  13. National Association of Testing Authorities (NATA), Specific Accreditation Criteria: ISO/IEC 17025 Application Document, Manufactured Goods - Annex, Physical performance testing - General in, National Association of Testing Authorities (NATA), Australia, 2018
  14. Singapore Accreditation Council, Technical Guide 2: A Guide on Measurement Uncertainty in Chemical & Microbiological Analysis, in, Singapore Accreditation Council, Singapore, 2019
  15. Eurachem, EURACHEM/CITAC Guide Quantifying Uncertainty in Analytical Measurement, in, 2012

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