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Antioxidant and Antimicrobial Screening of Endophytic Fungi Culture Filtrate from Purwoceng (Pimpinella alpina Molk) Leaf

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia

Received: 21 Apr 2020; Revised: 13 Aug 2020; Accepted: 7 Sep 2020; Published: 30 Sep 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
This is a preliminary study to determine the bioactivity potential of purwoceng leaf endophytic fungal metabolites. Endophytic fungi were isolated from purwoceng leaf and their secondary metabolite from culture filtrate were subjected to identify the antimicrobial, antioxidant, and phytochemical screening. The antioxidant activity was screened by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antimicrobial activity was screened using a good agar method toward Salmonella typhi, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, dan Candida albicans. This study obtained five distinctive endophytic fungi isolates named A, B, C, D, and E. The endophytic fungal culture filtrate of C has the most extensive antimicrobial activity with phytochemical screening showing alkaloids, saponins, and terpenoids. The antioxidant potential of all culture filtrates seemed low because the DPPH amount was interfered with by pigment compounds. Culture filtrate of fungi A showed the highest antioxidant activity and contained phenolic and alkaloid compounds.
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Keywords: purwoceng; endophyte; fungi; antioxidant; antimicrobial
Funding: Diponegoro University

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  1. K. Heyne, Tumbuhan berguna Indonesia, Yayasan Sarana Wana Jaya, Departemen Kehutanan, Jakarta, 1988
  2. Ireng Darwati, Ika Roostika, Status penelitian purwoceng (Pimpinella alpina Molk.) di Indonesia, Buletin Plasma Nutfah, 12, 1, (2016), 9-15 http://dx.doi.org/10.21082/blpn.v12n1.2006.p9-15
  3. Agustina D. R. Nurcahyanti, Issam J. Nasser, Frank Sporer, Bernhard Wetterauer, Ireng D. Kadarso, Jurgen Reichling, Michael Wink, Essential oil composition, in vivo antioxidant, and antimicrobial activities of Pimpinella pruatjan from West Java, Indonesia, The Natural Products Journal, 8, 1, (2018), 61-69 https://doi.org/10.2174/2210315507666170620093210
  4. Gary Strobel, Bryn Daisy, Bioprospecting for Microbial Endophytes and Their Natural Products, Microbiology and Molecular Biology Reviews, 67, 4, (2003), 491 https://doi.org/10.1128/MMBR.67.4.491-502.2003
  5. Wildiani Wilson, Yekti Asih Pawestri, Langkah Sembiring, Isolasi, karakterisasi dan skrining antimikroba bakteri endofit tanaman Purwoceng (Pimpinella pruatjan Molk), Jurnal Labora Medika, 1, 1, (2017), 1-6
  6. Yekti Asih Purwestri, Nur’aini Kartikasari, Sartika Gunawan Putri, Wildiani Wilson, Langkah Sembiring, Metabolic profiling of endophytic bacteria from Purwoceng (Pimpinella pruatjan Molkend) root and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, AIP Conference Proceedings, 2016 https://doi.org/10.1063/1.4953537
  7. Eris Septiana, Fauzy Rachman, Yatri Hapsari, Yadi Yadi, Bustanussalam Bustanussalam, Siti Irma Rahmawati, Partomuan Simanjuntak, The potential of Endophytic Fungal Extract Isolated from Cinnamon (Cinnamomum burmannii) as Antidiabetic and Antioxidant, Jurnal Kimia Sains dan Aplikasi, 22, 6, (2019), 275-282 https://doi.org/10.14710/jksa.22.6.275-282
  8. R. Abass Tolulope, Adeleye Isaac Adeyemi, M. Adongbede Erute, T. Seriki Abiodun, Isolation and screening of endophytic fungi from three plants used in traditional medicine in Nigeria for antimicrobial activity, International Journal of Green Pharmacy (IJGP), 9, 1, (2015), 58-62 https://doi.org/10.4103/0973-8258.150929
  9. Jeffrey B. Harborne, Phytochemical Methods, 3Island Press, 1980
  10. Akanksha Bhardwaj, Deeksha Sharma, Nitesh Jadon, Pavan Kumar Agrawal, Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus roxburghii, Archives of Clinical Microbiology, 6, 3, (2015), 1-9
  11. Sudipta Roy, Debdulal Banerjee, Isolation of antimicrobial compound by endophytic bacteria from Vinca rosea, International Journal of Current Research, 5, (2010), 47-51
  12. Joanne M. Willey, Linda Sherwood, Christopher J. Woolverton, Prescott, Harley, and Klein's Microbiology, 7th ed., McGraw-Hill Higher Education, 2008
  13. Paul M. Dewick, Medicinal Natural Products: A Biosynthetic Approach, Wiley, 1997
  14. Patrik C. Eklund, Otto K. Långvik, Johan P. Wärnå, Tapio O. Salmi, Stefan M. Willför, Rainer E. Sjöholm, Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans, Organic & Biomolecular Chemistry, 3, 18, (2005), 3336-3347 http://dx.doi.org/10.1039/B506739A
  15. Hiroe Kikuzaki, Masashi Hisamoto, Kanae Hirose, Kayo Akiyama, Hisaji Taniguchi, Antioxidant Properties of Ferulic Acid and Its Related Compounds, Journal of Agricultural and Food Chemistry, 50, 7, (2002), 2161-2168 https://doi.org/10.1021/jf011348w
  16. Prasanna Kumara, Sunil K, Arun Kumar B, Determination of DPPH Free Radical Scavenging Activity by RP-HPLC, Rapid Sensitive Method for the Screening of Berry Fruit Juice Freeze Dried Extract, Natural Products Chemistry & Research, 6, 5, (2018), 1000341 https://doi.org/10.4172/2329-6836.1000341
  17. Lars Müller, Kathleen Theile, Volker Böhm, In vitro antioxidant activity of tocopherols and tocotrienols and comparison of vitamin E concentration and lipophilic antioxidant capacity in human plasma, Molecular Nutrition & Food Research, 54, 5, (2010), 731-742 https://doi.org/10.1002/mnfr.200900399
  18. Donghong Liu, John Shi, Alejandra Colina Ibarra, Yukio Kakuda, Sophia Jun Xue, The scavenging capacity and synergistic effects of lycopene, vitamin E, vitamin C, and β-carotene mixtures on the DPPH free radical, LWT - Food Science and Technology, 41, 7, (2008), 1344-1349 https://doi.org/10.1016/j.lwt.2007.08.001
  19. Daniel M. Cornforth, Kevin R. Foster, Competition sensing: the social side of bacterial stress responses, Nature Reviews Microbiology, 11, 4, (2013), 285-293 https://doi.org/10.1038/nrmicro2977

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