Determination of Shelf Life of Herbal Products from the Combination of Stevia rebaudiana, Curcuma zanthorrhiza and Honey (Stekurmin MD) through the Accelerated Shelf Life Test (ASLT) Method

*Yohanes Martono scopus  -  Department of Chemistry, Faculty of Science and Mathematics, Satya Wacana Christian University, Indonesia
Fidelia Novitasari  -  Department of Chemistry, Satya Wacana Christian University, Indonesia
November Rianto Aminu  -  Department of Chemistry, Satya Wacana Christian University, Indonesia
Received: 21 Apr 2020; Revised: 1 Sep 2020; Accepted: 7 Sep 2020; Published: 30 Sep 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0

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Section: Research Articles
Language: EN
Statistics: 137 40
Abstract
Stekurmin MD is a syrup preparation formulated from the extract of Stevia rebaudiana leaves, temu lawak (Curcuma zanthorrhiza), and honey. One of the active compounds in Stekurmin MD products is phenolic and flavonoid compounds in which the active phenolic and flavonoid compounds can be degraded during the storage period. This study aimed to determine the shelf life of Stekurmin MD products based on the degradation of phenolic compounds and flavonoids. The Accelerated Shelf Life Test (ASLT) method was used in determining the shelf life kinetics of this product. Phenolic and flavonoid concentrations were determined using the UV-Vis spectrophotometric method with the gallic acid standard for phenolic and quercetin standard for flavonoids. Degradation of phenolic and flavonoid compounds was determined every 7 days during 35 days of storage at 25, 35, 45, and 55°C ± 2°C. In natural ingredients, there is a multi-mechanism reaction. The shelf life of Stekurmin MD products based on the degradation of phenolic and flavonoid active ingredients at room temperature were 39.909 days and 23.53 hours, respectively.
Keywords: Accelerated Shelf Life Test (ASLT); Stevia rebaudiana; shelf-life; phenolic; flavonoids

Article Metrics:

  1. Zhewen Fan, Lanju Zhang, One- and Two-Stage Arrhenius Models for Pharmaceutical Shelf Life Prediction, Journal of Biopharmaceutical Statistics, 25, 2, (2015), 307-316 https://doi.org/10.1080/10543406.2014.972743
  2. Panitia Farmakope Indonesia, Farmakope Indonesia, Departemen Kesehatan Republik Indonesia, 2014
  3. Yohanes Martono, J. Muninggar, Optimasi Proses Produksi Minuman Fungsional Stevia Sebagai Obat Herbal Terstandar (OHT) Yang Berkhasiat Antidiabetes, Universitas Kristen Satya Wacana, Salatiga, 2017
  4. Subarkah Arif, Susinggih Wijana, Arie Febrianto Mulyadi, Pendugaan Umur Simpan Minuman Sari Buah Sirsak (Annona muricata L.) berdasarkan Parameter Kerusakan Fisik dan Kimia dengan Metode Accelerated Shelf Life Testing (ASLT), Industria: Jurnal Teknologi dan Manajemen Agroindustri, 4, 2, (2016), 89-96
  5. Rika Diananing Putri, Ratih Yuniastri, Pendugaan Umur Simpan Menggunakan Metode Accelerated Shelf Life Test (ASLT) Model Arrhenius pada Jamu “Sari Rapet Super”, Journal of Food Technology and Agroindustry, 1, 2, (2019), 37-42 https://doi.org/10.24929/jfta.v1i2.781
  6. Abisatya Widya Swadana, Sudarminto Setyo Yuwono, Pendugaan Umur Simpan Minuman Berperisa Apel Menggunakan Metode Accelerated Shelf Life Testing (ASLT) dengan Pendekatan Arrhenius, Jurnal Pangan dan Agroindustri, 2, 3, (2014), 203-213
  7. J Prchalová, H Čížková, R Ševčík, K Hanušová, A Rajchl, Evaluation of shelf-life of fruit baby food, Agronomy Research, 14, 2, (2016), 556-568
  8. Yohanes Martono, Dewi K. A .K. H., Optimization of Production Process Stevia Beverages with Antidiabetic Activity, The 2nd International Conference of the Indonesian Chemical Society, Surakarta, 2013
  9. Yohanes Martono, Sugeng Riyanto, Abdul Rohman, Sudibyo Martono, Improvement method of fast and isocratic RP-HPLC analysis of major diterpene glycoside from Stevia rebaudiana leaves, AIP Conference Proceedings, 1755, 1, (2016), 080001 https://doi.org/10.1063/1.4958509
  10. Sri Mulyanti, Optimasi Klarifikasi Ekstrak Air Stevia rebaudiana (Bert.) dengan Metode Elektrokoagulasi Menggunakan Elektroda Al dan Zn, Departemen Fisika, Universitas Kristen Satya Wacana, Salatiga, 2018
  11. Ki Won Lee, Young Jun Kim, Hyong Joo Lee, Chang Yong Lee, Cocoa Has More Phenolic Phytochemicals and a Higher Antioxidant Capacity than Teas and Red Wine, Journal of Agricultural and Food Chemistry, 51, 25, (2003), 7292-7295 https://doi.org/10.1021/jf0344385
  12. Jianguo Chen, Per Bendix Jeppesen, Reziwanggu Abudula, Stig E. U. Dyrskog, Michele Colombo, Kjeld Hermansen, Stevioside does not cause increased basal insulin secretion or β-cell desensitization as does the sulphonylurea, glibenclamide: Studies in vitro, Life Sciences, 78, 15, (2006), 1748-1753 https://doi.org/10.1016/j.lfs.2005.08.012
  13. Pramudita Dwi Apsari, Hari Susanti, Perbandingan Kadar Fenolik Total Ekstrak Metanol Kelopak Merah dan Ungu Bunga Rosella (Hibiscus sabdariffa Linn) secara Spektrofotometri, Prosiding Seminar Nasional Home Care, 2011
  14. Sri Wahdaningsih, Subagus Wahyuono, Sugeng Riyanto, Retno Murwanti, Penetapan Kadar Fenolik Total dan Flavonoid Total Ekstrak Metanol dan Fraksi Etil Asetat Kulit Buah Naga Merah (Hylocereus Polyrhizus (Fac Weber) Britton dan Rose), PHARMACON, 6, 3, (2017), 295-301
  15. Khadijah Khadijah, Ahmad Muchsin Jayali, Sudir Umar, Iin Sasmita, Penentuan Total Fenolik dan Aktivitas Antioksidan Ekstrak Etanolik Daun Samama (Anthocephalus macrophylus) Asal Ternate, Maluku Utara, Jurnal Kimia Mulawarman, 15, 1, (2017), 11-18 https://doi.org/10.30872/jkm.v15i1.495
  16. Xirley Pereira Nunes, Fabrício Souza Silva, Jackson Roberto Guedes da S Almeida, José Maria Barbosa Filho, Julianeli Tolentino de Lima, Luciano Augusto de Araújo Ribeiro, Lucindo José Quintans Júnior, Biological oxidations and antioxidant activity of natural products, in: V. Rao (Ed.) Phytochemicals as Nutraceuticals; Global Approaches to Their Role in Nutrition and Health, INTECH Open Access Publisher, New York, 2012
  17. Jeremy S. Barnes, Characterization of Flavonoid Reactivities and Degradation Products Using Online Continuous Flow Kinetic Measurements and Higher Order Mass Spectrometry, PhD Dissertations, Departement of Chemistry, University of Texas at Arlington, Arlington, 2014
  18. Sneha Sehwag, Rohit Upadhyay, Madhusweta Das, Optimization and multivariate accelerated shelf life testing (MASLT) of a low glycemic whole jamun (Syzygium cumini L.) confection with tailored quality and functional attributes, Journal of Food Science and Technology, 55, 12, (2018), 4887-4900 https://doi.org/10.1007/s13197-018-3423-4
  19. Guy B. Marin, Gregory S. Yablonsky, Denis Constales, Kinetics of chemical reactions: Decoding complexity, John Wiley & Sons, 2019
  20. Arief Fadillah, Agung Rahmadani, Laode Rijai, Analisis Kadar Total Flavonoid dan Uji Aktivitas Antioksidan Ekstrak Daun Kelubut (Passiflora foetida L.), Proceeding of Mulawarman Pharmaceuticals Conferences, 2017 https://doi.org/10.25026/mpc.v5i1.217
  21. Dyah Nur Azizah, Endang Kumolowati, Fahrauk Faramayuda, Penetapan kadar flavonoid metode AlCl3 pada ekstrak metanol kulit buah kakao (Theobroma cacao L.), Kartika: Jurnal Ilmiah Farmasi, 2, 2, (2014), 45-49 http://dx.doi.org/10.26874/kjif.v2i2.14
  22. Hongbin Zhu, Yuzhi Wang, Yuxuan Liu, Yalin Xia, Tian Tang, Analysis of Flavonoids in Portulaca oleracea L. by UV–Vis Spectrophotometry with Comparative Study on Different Extraction Technologies, Food Analytical Methods, 3, 2, (2010), 90-97 https://doi.org/10.1007/s12161-009-9091-2
  23. Harmita Harmita, Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya, Pharmaceutical Sciences and Research (PSR), 1, 3, (2004), 117-135 http://dx.doi.org/10.7454/psr.v1i3.3375
  24. Jing Wang, Xin-Huai Zhao, Degradation kinetics of fisetin and quercetin in solutions as effected by pH, temperature and coexisted proteins, Journal of the Serbian Chemical Society, 81, 3, (2016), 243-253 https://doi.org/10.2298/JSC150706092W
  25. Marco Ciulu, Rosa Quirantes-Piné, Nadia Spano, Gavino Sanna, Isabel Borrás-Linares, Antonio Segura-Carretero, Evaluation of new extraction approaches to obtain phenolic compound-rich extracts from Stevia rebaudiana Bertoni leaves, Industrial Crops and Products, 108, (2017), 106-112 https://doi.org/10.1016/j.indcrop.2017.06.024
  26. Amriteswori Rajbhandari, Margaret F. Roberts, The Flavonoids of Stevia rebaudiana, Journal of Natural Products, 46, 2, (1983), 194-195 https://doi.org/10.1021/np50026a010