1Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
2Chemistry Department, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Indonesia
BibTex Citation Data :
@article{JKSA29767, author = {Martha Sari and Dirayanti Dirayanti and Eris Septiana and Bustanussalam Bustanussalam and Apon Zaenal Mustopa}, title = {The Combination of Carbon Source and the Addition of Phenylacetic Acid (PAA) to Growth Medium Penicillium chrysogenum to Enhance of Penicillin (Pen G) Production}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {23}, number = {9}, year = {2020}, keywords = {Carbon source; PAA; P. chrysogenum; Pen G; Disk diffusion}, abstract = { The nutrition factor governs the growth and production of Penicillin G (Pen G) by Penicillium chrysogenum in a broth medium. Proper nutrition can improve Pen G antibiotic production. In this research, the optimal condition for Pen G production from P. chrysogenum in a standard culture medium and various carbon sources medium (glucose, lactose, maltose, and sucrose) were done for ten days. Phenylacetic Acid (PAA) precursor at 0.0 – 0.6 gL -1 (increment 0.1) was used to improve Pen G production. The Pen G was detected by HPLC, compared with the standard (Penicillin G Sodium Salt). The results showed that the PDB standard medium and lactose medium (150 rpm, at 30°C) produced 0.425 gL -1 and 0.107 gL -1 Pen G. Addition of 0.6 gL -1 PAA improved the Pen G production up to 0.045 gL -1 in the PDB medium, become the final concentration of 0.470 gL -1 and 2.460 gL -1 in the lactose medium, become the final concentration of 2.565 gL -1 . The antibiotic’s activity against the pathogenic bacteria, i.e., B. subtilis , S. aureus , and S. typhi employing the disk diffusion method, has been done. The TLC method’s detection of the potential Pen G spots was conducted with ethyl acetate: distilled water: acetic acid (60:20:20) as the mobile phase. The Pen G extracts could inhibit the growth of all tested bacteria in Rf 0.65. This study informs the proper combination of carbon source and precursor effects and increases the bioproduction of Pen G from P. chrysogenum . }, issn = {2597-9914}, pages = {312--318} doi = {10.14710/jksa.23.9.312-318}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/29767} }
Refworks Citation Data :
The nutrition factor governs the growth and production of Penicillin G (Pen G) by Penicillium chrysogenum in a broth medium. Proper nutrition can improve Pen G antibiotic production. In this research, the optimal condition for Pen G production from P. chrysogenum in a standard culture medium and various carbon sources medium (glucose, lactose, maltose, and sucrose) were done for ten days. Phenylacetic Acid (PAA) precursor at 0.0 – 0.6 gL-1 (increment 0.1) was used to improve Pen G production. The Pen G was detected by HPLC, compared with the standard (Penicillin G Sodium Salt). The results showed that the PDB standard medium and lactose medium (150 rpm, at 30°C) produced 0.425 gL-1 and 0.107 gL-1 Pen G. Addition of 0.6 gL-1 PAA improved the Pen G production up to 0.045 gL-1 in the PDB medium, become the final concentration of 0.470 gL-1 and 2.460 gL-1 in the lactose medium, become the final concentration of 2.565 gL-1. The antibiotic’s activity against the pathogenic bacteria, i.e., B. subtilis, S. aureus, and S. typhi employing the disk diffusion method, has been done. The TLC method’s detection of the potential Pen G spots was conducted with ethyl acetate: distilled water: acetic acid (60:20:20) as the mobile phase. The Pen G extracts could inhibit the growth of all tested bacteria in Rf 0.65. This study informs the proper combination of carbon source and precursor effects and increases the bioproduction of Pen G from P. chrysogenum.
Article Metrics:
Last update:
Last update: 2024-11-21 20:38:48
As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.
Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).
Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.
The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.
Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners
JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.
Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
