DOI: https://doi.org/10.14710/jksa.26.5.160-165

Biosensor based on Cellulose Acetate/Glutaraldehyde Membrane Electrodes for detection of organophosphorus pesticides

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, 93132, Indonesia

2Magister program of Chemistry, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, 93132, Indonesia

3Department of Physics, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, 93132, Indonesia

4 Department of Biology, Faculty of Biology, Gadjah Mada University, Yogyakarta, 55281, Indonesia

View all affiliations
Received: 20 Jan 2023; Revised: 4 Jun 2023; Accepted: 22 Jun 2023; Published: 31 Jul 2023.
Open Access Copyright 2023 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract

In recent years, sensor applications have been critical in many fields, especially food safety and pesticides. Organophosphorus pesticides (OPPs) can be detected using a potentiometric biosensor with a membrane electrode made of a new natural material based on cellulose acetate (CA). Acetylcholinesterase was immobilized to 15% modified CA membrane electrodes using glutaraldehyde (GTA) as crosslinking agent and gold (Au) electrode. An indirect method used an acetylthiocholine chloride (ATCl) substrate to find OPPs like chlorpyrifos, profenophos, and diazinon. The working electrode was an CA/GTA membrane electrode, and the reference electrode was an Ag/AgCl electrode, whose potential value was measured with a potentiometer. The surface morphology of the biosensor membrane was investigated using scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDX). It showed that the CA membrane has a smooth, porous surface and is very dense, and its structure consists of 71.27% carbon (C) and 28.73% oxygen (O) with an average diameter of 562.33 nm. A potentiometric biosensor based on AChE inhibition for the detection of OPPs showed a limit of detection (LoD) of 1×10−6 μg/L with a linearity range of 1×10−6–1.0 μg/L. The %inhibition value for the chlorpyrifos pesticide was 14.44 to 73.08%, profenophos was 11.98 to 77.98%, and diazinon was 18.58 to 83.27%. Therefore, higher inhibitor concentrations (OPPs) have a greater ability to prevent the AChE enzyme from breaking down the acetylcholine substrate. The biosensor with the CA membrane has a wide linearity range and a low detection limit. The potentiometer rapidly detects pesticide residues.

Note: This article has supplementary file(s).

Fulltext View|Download |  Copyright Transfer Agreement
Inhibition of Organophosphate Pesticide against Acetylcholinesterase based on Immobilized in Cellulose Acetate Membrane Electrodes
Subject COPYRIGHT TRANSFER AGREEMENT
Type Copyright Transfer Agreement
  Download (666KB)    Indexing metadata
Keywords: acetylcholinesterase; biosensor; cellulose acetate; organophosphate; potentiometric
Funding: Ministry of Education, Culture, Research, and Technology (Kemdikbudristek) of the Republic of Indonesia in the years 2021–2022

Article Metrics:

Article Info
Section: Research Articles
Language : EN
Recent articles
Molecular Docking of Active Compounds of Syzygium myrtifolium Walp. Leaves on Leukotriene A4 Hydrolase Receptors as Colorectal Anticancer Synthesis of Molecularly Imprinted Membrane Glucose for Selective Membrane Transport The potential of Myricitrin, a Flavonoid Compound in Eugenia polyantha from Indonesia, as an Antiviral Drug for SARS-Cov-2 through the Molecular Docking Analysis More recent articles
Most cited articles
Aktivitas Antioksidan dan Antibakteri Produk Fermentasi Susu Kedelai dan Whey Tahu menggunakan Bakteri Asam Laktat Komersial Skrining Metabolit Sekunder Bakteri Endofit yang Berfungsi sebagai Antidiabetes dari Daun Mimba (Azadirachta Indica) Preparasi Katalis Zeolit Alam Asam sebagai Katalis dalam Proses Pirolisis Katalitik Polietilena Dimerisasi Metilisoeugenol dengan Katalis HCl Isolasi Bakteri Termofilik Sumber Air Panas Gedongsongo dengan Media Pengaya MB (Minimal Broth) dan TS (Taoge Sukrosa) serta Identifikasi Fenotip dan Genotip More cited articles
  1. Sassolas Audrey, Prieto-Simón Beatriz, Marty Jean-Louis, Biosensors for pesticide detection: new trends, American Journal of Analytical Chemistry, 3, 3, (2012), http://dx.doi.org/10.4236/ajac.2012.33030
  2. C. S. Pundir, Ashish Malik, Preety, Bio-sensing of organophosphorus pesticides: A review, Biosensors and Bioelectronics, 140, (2019), 111348 https://doi.org/10.1016/j.bios.2019.111348
  3. Brajesh K. Singh, Organophosphorus-degrading bacteria: ecology and industrial applications, Nature Reviews Microbiology, 7, (2009), 156-164 https://doi.org/10.1038/nrmicro2050
  4. Paul Yager, Thayne Edwards, Elain Fu, Kristen Helton, Kjell Nelson, Milton R. Tam, Bernhard H. Weigl, Microfluidic diagnostic technologies for global public health, Nature, 442, (2006), 412-418 https://doi.org/10.1038/nature05064
  5. S. M. Zakir Hossain, Roger E. Luckham, Meghan J. McFadden, John D. Brennan, Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples, Analytical Chemistry, 81, 21, (2009), 9055-9064 https://doi.org/10.1021/ac901714h
  6. Mohamed EI Badawy, Ahmed F. El-Aswad, Bioactive paper sensor based on the acetylcholinesterase for the rapid detection of organophosphate and carbamate pesticides, International Journal of Analytical Chemistry, 2014, (2014), 536823 https://doi.org/10.1155/2014/536823
  7. C. Anagnostopoulos, G. E. Miliadis, Development and validation of an easy multiresidue method for the determination of multiclass pesticide residues using GC–MS/MS and LC–MS/MS in olive oil and olives, Talanta, 112, (2013), 1-10 https://doi.org/10.1016/j.talanta.2013.03.051
  8. Yousheng Huang, Ting Shi, Xiang Luo, Hualiang Xiong, Fangfang Min, Yi Chen, Shaoping Nie, Mingyong Xie, Determination of multi-pesticide residues in green tea with a modified QuEChERS protocol coupled to HPLC-MS/MS, Food Chemistry, 275, (2019), 255-264 https://doi.org/10.1016/j.foodchem.2018.09.094
  9. Nho-Eul Song, Jun Young Lee, Ahmad Rois Mansur, Hae Won Jang, Min-Cheol Lim, Yunyeol Lee, Miyoung Yoo, Tae Gyu Nam, Determination of 60 pesticides in hen eggs using the QuEChERS procedure followed by LC-MS/MS and GC-MS/MS, Food Chemistry, 298, (2019), 125050 https://doi.org/10.1016/j.foodchem.2019.125050
  10. Meng Li, Da-Wei Li, Guangli Xiu, Yi-Tao Long, Applications of screen-printed electrodes in current environmental analysis, Current Opinion in Electrochemistry, 3, 1, (2017), 137-143 https://doi.org/10.1016/j.coelec.2017.08.016
  11. Yawen Rong, Huanhuan Li, Qin Ouyang, Shujat Ali, Quansheng Chen, Rapid and sensitive detection of diazinon in food based on the FRET between rare-earth doped upconversion nanoparticles and graphene oxide, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 239, (2020), 118500 https://doi.org/10.1016/j.saa.2020.118500
  12. Duygu Akyüz, Atıf Koca, An electrochemical sensor for the detection of pesticides based on the hybrid of manganese phthalocyanine and polyaniline, Sensors and Actuators B: Chemical, 283, (2019), 848-856 https://doi.org/10.1016/j.snb.2018.11.155
  13. Silvana Andreescu, Jean-Louis Marty, Twenty years research in cholinesterase biosensors: from basic research to practical applications, Biomolecular Engineering, 23, 1, (2006), 1-15 https://doi.org/10.1016/j.bioeng.2006.01.001
  14. M. D. Luque De Castro, M. C. Herrera, Enzyme inhibition-based biosensors and biosensing systems: questionable analytical devices, Biosensors and Bioelectronics, 18, 2-3, (2003), 279-294 https://doi.org/10.1016/S0956-5663(02)00175-6
  15. Karolina Sipa, Mariola Brycht, Andrzej Leniart, Paweł Urbaniak, Agnieszka Nosal-Wiercińska, Bartłomiej Pałecz, Sławomira Skrzypek, β–Cyclodextrins incorporated multi-walled carbon nanotubes modified electrode for the voltammetric determination of the pesticide dichlorophen, Talanta, 176, (2018), 625-634 https://doi.org/10.1016/j.talanta.2017.07.084
  16. Dorothee Grieshaber, Robert MacKenzie, Janos Vörös, Erik Reimhult, Electrochemical biosensors-sensor principles and architectures, Sensors, 8, 3, (2008), 1400-1458 https://doi.org/10.3390/s80314000
  17. Nidhi Chauhan, Chandra Shekhar Pundir, An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides, Analytica Chimica Acta, 701, 1, (2011), 66-74 https://doi.org/10.1016/j.aca.2011.06.014
  18. Miroslav Pohanka, Kamil Musilek, Kamil Kuca, Progress of biosensors based on cholinesterase inhibition, Current medicinal chemistry, 16, 14, (2009), 1790-1798 http://dx.doi.org/10.2174/092986709788186129
  19. Yvan Boublik, Pascale Saint-Aguet, Andrée Lougarre, Muriel Arnaud, François Villatte, Sandino Estrada-Mondaca, Didier Fournier, Acetylcholinesterase engineering for detection of insecticide residues, Protein Engineering, Design and Selection, 15, 1, (2002), 43-50 https://doi.org/10.1093/protein/15.1.43
  20. Vikas Dhull, Anjum Gahlaut, Neeraj Dilbaghi, Vikas Hooda, Acetylcholinesterase biosensors for electrochemical detection of organophosphorus compounds: a review, Biochemistry Research International, 2013, (2013), 731501 https://doi.org/10.1155/2013/731501
  21. Graziella L. Turdean, Ionel Catalin Popescu, Liviu Oniciu, Daniel R. Thevenot, Sensitive detection of organophosphorus pesticides using a needle type amperometric acetylcholinesterase-based bioelectrode. Thiocholine electrochemistry and immobilised enzyme inhibition, Journal of Enzyme Inhibition and Medicinal Chemistry, 17, 2, (2002), 107-115 https://doi.org/10.1080/14756360290026469
  22. Derya Y. Koseoglu-Imer, Nadir Dizge, Ismail Koyuncu, Enzymatic activation of cellulose acetate membrane for reducing of protein fouling, Colloids and Surfaces B: Biointerfaces, 92, (2012), 334-339 https://doi.org/10.1016/j.colsurfb.2011.12.013
  23. Jamaliah Aburabie, Boor Lalia, Raed Hashaikeh, Proton conductive, low methanol crossover cellulose-based membranes, Membranes, 11, 7, (2021), 539 https://doi.org/10.3390/membranes11070539
  24. Shuo Wu, Xiaoqin Lan, Wei Zhao, Yuping Li, Lihui Zhang, Hainan Wang, Mei Han, Shengyang Tao, Controlled immobilization of acetylcholinesterase on improved hydrophobic gold nanoparticle/Prussian blue modified surface for ultra-trace organophosphate pesticide detection, Biosensors and Bioelectronics, 27, 1, (2011), 82-87 https://doi.org/10.1016/j.bios.2011.06.020
  25. T. H. Vignesh Kumar, Ashok K. Sundramoorthy, Electrochemical biosensor for methyl parathion based on single-walled carbon nanotube/glutaraldehyde crosslinked acetylcholinesterase-wrapped bovine serum albumin nanocomposites, Analytica Chimica Acta, 1074, (2019), 131-141 https://doi.org/10.1016/j.aca.2019.05.011
  26. Mashuni, L. O. A. N. Ramadhan, M. Jahiding, Herniati, Analysis of diazinon pesticide using potentiometric biosensor based on enzyme immobilized cellulose acetate membrane in gold electrode, IOP Conference Series: Materials Science and Engineering, 2016 https://doi.org/10.1088/1757-899X/107/1/012013
  27. Mashuni, L. O. A. N. Ramadhan, Y. Marlina, M. Jahiding, Optimization of Cellulose Acetate Membrane in Biosensor Electrode of Profenofos Pesticide, The 5th Annual Basic Science International Conference, 2015
  28. Nidhi Chauhan, Jagriti Narang, C. S. Pundir, Immobilization of rat brain acetylcholinesterase on ZnS and poly (indole-5-carboxylic acid) modified Au electrode for detection of organophosphorus insecticides, Biosensors and Bioelectronics, 29, 1, (2011), 82-88 https://doi.org/10.1016/j.bios.2011.07.070
  29. Subramanian Viswanathan, Hanna Radecka, Jerzy Radecki, Electrochemical biosensor for pesticides based on acetylcholinesterase immobilized on polyaniline deposited on vertically assembled carbon nanotubes wrapped with ssDNA, Biosensors and Bioelectronics, 24, 9, (2009), 2772-2777 https://doi.org/10.1016/j.bios.2009.01.044
  30. Joseph Wang, Electrochemical glucose biosensors, Chemical Reviews, 108, 2, (2008), 814-825 https://doi.org/10.1021/cr068123a
  31. Avi A. Weinbroum, Pathophysiological and clinical aspects of combat anticholinesterase poisoning, British Medical Bulletin, 72, 1, (2004), 119-133 https://doi.org/10.1093/bmb/ldh038
  32. Lin Zhang, Aidong Zhang, Dan Du, Yuehe Lin, Biosensor based on Prussian blue nanocubes/reduced graphene oxide nanocomposite for detection of organophosphorus pesticides, Nanoscale, 4, (2012), 4674-4679 https://doi.org/10.1039/C2NR30976A
  33. Neetu Jha, S. Ramaprabhu, Development of MWNT based disposable biosensor on glassy Carbon electrode for the detection of organophosphorus nerve agents, Journal of Nanoscience and Nanotechnology, 9, 9, (2009), 5676-5680 https://doi.org/10.1166/jnn.2009.1200

Last update:

No citation recorded.

Last update: 2024-05-07 21:19:55

No citation recorded.