skip to main content

Rosella Petal Extract (Hibiscus sabdariffa Linn.) as a Reducing Agent in Silver Nanoparticle Synthesis and Its Antibacterial Activity

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Tanjungpura, Pontianak, Indonesia

Received: 24 Sep 2023; Revised: 11 Dec 2023; Accepted: 21 Dec 2023; Published: 23 Dec 2023.
Open Access Copyright 2023 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
kelopak Hibiscus sabdarifa Linn. dlakukan ekstraksi dengan metanol. Ekstrak digunakan sebagai reduktor pada sintesis nanopartikel perak (NPAg). Karakterisasi dilakukan terhadap NPAg dan dilakukan uji antibakteri terhadap S. aereus dan E. coli
Abstract

Silver nanoparticles have superior properties that attract much attention because they can increase their effectiveness and efficiency compared to their macroscopic size. Silver nanoparticles have several benefits, one of which is as an antibacterial agent. Synthesis of silver nanoparticles was carried out by reducing silver ions (Ag+) to uncharged silver (Ag0) using a reducing agent from Hibiscus sabdariffa Linn. petal extract. This research aims to synthesize silver nanoparticles by studying several parameters that influence their formation, such as the pH of Hibiscus sabdariffa Linn. petal extract, reaction time, silver nitrate concentration, and Hibiscus sabdariffa Linn. petal extract concentration. The silver nanoparticles formed were then tested for their antibacterial activity. The experimental results showed that the best synthesis was performed using Hibiscus sabdariffa Linn. petal extract with pH 10, reaction time of 45 minutes, silver nitrate concentration of 1×10-4 M, and Hibiscus sabdariffa Linn. petal extract concentration of 0.004%. The synthesized silver nanoparticles, examined through a transmission electron microscope, exhibited a size distribution ranging from 2 to 26 nm with an average size of 12 ± 3 nm, which was stable with a storage time of 3 months. Silver nanoparticles showed antibacterial activity against Staphylococcus aureus. Hibiscus sabdariffa Linn. petal extract is a promising reducing agent for producing antibacterial silver nanoparticles, showcasing its potential in this application.

Fulltext View|Download
Keywords: Silver nanoparticles; Hibiscus sabdariffa Linn.; antibacterial

Article Metrics:

  1. Sourav Bhattacharjee, DLS and zeta potential – What they are and what they are not?, Journal of Controlled Release, 235, (2016), 337-351 https://doi.org/10.1016/j.jconrel.2016.06.017
  2. Xi-Feng Zhang, Zhi-Guo Liu, Wei Shen, Sangiliyandi Gurunathan, Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches, International Journal of Molecular Sciences, 17, 9, (2016), 1534 https://doi.org/10.3390/ijms17091534
  3. Dattu Singh, Vandana Rathod, Shivaraj Ninganagouda, Jyothi Hiremath, Ashish Kumar Singh, Jasmine Mathew, Optimization and Characterization of Silver Nanoparticle by Endophytic Fungi Penicillium sp. Isolated from Curcuma longa (Turmeric) and Application Studies against MDR E. coli and S. aureus, Bioinorganic Chemistry and Applications, 2014, (2014), 408021 https://doi.org/10.1155/2014/408021
  4. Lilis Sulistiawaty, Sri Sugiarti, Noviyan Darmawan, Detection of Hg2+ Metal Ions Using Silver Nanoparticles Stabilized by Gelatin and Tween-20, Indonesian Journal of Chemistry, 15, 1, (2015), 1-8 https://doi.org/10.22146/ijc.21216
  5. Gusti Ayu Dewi Lestari, Iryanti Eka Suprihatin, James Sibarani, Sintesis Nanopartikel Perak (NPAg) Menggunakan Ekstrak Air Buah Andaliman (Zanthoxylum acanthopodium DC.) dan Aplikasinya pada Fotodegradasi Indigosol Blue, Jurnal Kimia Sains dan Aplikasi, 22, 5, (2019), 200-205 https://doi.org/10.14710/jksa.22.5.200-205
  6. Sang Hun Lee, Bong-Hyun Jun, Silver Nanoparticles: Synthesis and Application for Nanomedicine, International Journal of Molecular Sciences, 20, 4, (2019), 865 https://doi.org/10.3390/ijms20040865
  7. G. Krishna, V. Pranitha, Reeja Sundaram, M. A. Singara Charya, Biogenic Synthesis of Silver Nanoparticles and Their Applications, in: Functionalized Nanomaterials I, CRC Press, 2020,
  8. Gusrizal Gusrizal, Sri Juari Santosa, Eko Sri Kunarti, Bambang Rusdiarso, Dual function of p-hydroxybenzoic acid as reducing and capping agent in rapid and simple formation of stable silver nanoparticles, International Journal of ChemTech Research, 9, 9, (2016), 472-482
  9. Khairul Nazri Yusof, Siti Salwa Alias, Zawati Harun, Hatijah Basri, Faiz Hafeez Azhar, Parkia speciosa as Reduction Agent in Green Synthesis Silver Nanoparticles, ChemistrySelect, 3, 31, (2018), 8881-8885 https://doi.org/10.1002/slct.201801846
  10. Shabir Ahmad, Sidra Munir, Nadia Zeb, Asad Ullah, Behramand Khan, Javed Ali, Muhammad Bilal, Muhammad Omer, Muhammad Alamzeb, Syed Muhammad Salman, Green nanotechnology: A review on green synthesis of silver nanoparticles—An ecofriendly approach, International journal of nanomedicine, (2019), 5087-5107 https://doi.org/10.2147/IJN.S200254
  11. Sista Kameswara Srikar, Deen Dayal Giri, Dan Bahadur Pal, Pradeep Kumar Mishra, Siddh Nath Upadhyay, Green synthesis of silver nanoparticles: a review, Green and Sustainable Chemistry, 6, 1, (2016), 34-56 http://dx.doi.org/10.4236/gsc.2016.61004
  12. Anastasia Wheni Indrianingsih, Vita Taufika Rosyida, Wuri Apriyana, Septi Nur Hayati, Cici Darsih, Khoirun Nisa, Dwi Ratih, Antioxidant and antibacterial properties of bacterial cellulose— Indonesian plant extract composites for mask sheet, Journal of Applied Pharmaceutical Science, 10, 7, (2020), 037-042 http://dx.doi.org/10.7324/JAPS.2020.10705
  13. Irma Yunitasari, Agustina L. N. Aminin, Khairul Anam, Aktivitas Inhibisi α-Glukosidase dan Identifikasi Senyawa dalam Fraksi Aktif Bunga Rosella (Hibiscus Sabdariffa L.), Jurnal Kimia Sains dan Aplikasi, 18, 3, (2015), 110-115 https://doi.org/10.14710/jksa.18.3.110-115
  14. Khwaja Salahuddin Siddiqi, Azamal Husen, Rifaqat A. K. Rao, A review on biosynthesis of silver nanoparticles and their biocidal properties, Journal of Nanobiotechnology, 16, (2018), 14 https://doi.org/10.1186/s12951-018-0334-5
  15. V. V. Makarov, A. J. Love, O. V. Sinitsyna, S. S. Makarova, I. V. Yaminsky, M. E. Taliansky, N. O. Kalinina, “Green” Nanotechnologies: Synthesis of Metal Nanoparticles Using Plants, ActaNaturae, 6, 1, (2014), 35-44 https://doi.org/10.32607/20758251-2014-6-1-35-44
  16. Kiran Jadhav, Sharada Deore, Dinesh Dhamecha, Rajeshwari H R, Satveer Jagwani, Sunil Jalalpure, Raghvendra Bohara, Phytosynthesis of Silver Nanoparticles: Characterization, Biocompatibility Studies, and Anticancer Activity, ACS Biomaterials Science & Engineering, 4, 3, (2018), 892-899 https://doi.org/10.1021/acsbiomaterials.7b00707
  17. Zhiwen Xu, Cai Zhang, Xiang Wang, Dingbin Liu, Release Strategies of Silver Ions from Materials for Bacterial Killing, ACS Applied Bio Materials, 4, 5, (2021), 3985-3999 https://doi.org/10.1021/acsabm.0c01485
  18. Benjamin Le Ouay, Francesco Stellacci, Antibacterial activity of silver nanoparticles: A surface science insight, Nano Today, 10, 3, (2015), 339-354 https://doi.org/10.1016/j.nantod.2015.04.002
  19. A. J. Harborne, Phytochemical Methods A Guide to Modern Techniques of Plant Analysis, 3 ed., Springer Dordrecht, 1998,
  20. Amin Baghizadeh, Shahla Ranjbar, Vinod Kumar Gupta, Mohammad Asif, Shahram Pourseyedi, Mohammad J. Karimi, Reza Mohammadinejad, Green synthesis of silver nanoparticles using seed extract of Calendula officinalis in liquid phase, Journal of Molecular Liquids, 207, (2015), 159-163 https://doi.org/10.1016/j.molliq.2015.03.029
  21. Risa Nofiani, Alexandra J. Weisberg, Takeshi Tsunoda, Ruqiah Ganda Putri Panjaitan, Ridho Brilliantoro, Jeff H. Chang, Benjamin Philmus, Taifo Mahmud, Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts, International Journal of Microbiology, 2020, (2020), 8898631 https://doi.org/10.1155/2020/8898631
  22. V. Ravichandran, S. Vasanthi, S. Shalini, Syed Adnan Ali Shah, M. Tripathy, Neeraj Paliwal, Green synthesis, characterization, antibacterial, antioxidant and photocatalytic activity of Parkia speciosa leaves extract mediated silver nanoparticles, Results in Physics, 15, (2019), 102565 https://doi.org/10.1016/j.rinp.2019.102565
  23. Naheed Ahmad, Seema Sharma, Md K. Alam, V. N. Singh, S. F. Shamsi, B. R. Mehta, Anjum Fatma, Rapid synthesis of silver nanoparticles using dried medicinal plant of basil, Colloids and Surfaces B: Biointerfaces, 81, 1, (2010), 81-86 https://doi.org/10.1016/j.colsurfb.2010.06.029
  24. M. Sundarrajan, A. Jeelani, V. Santhanam, S. Durgadevi, S. Abirami, Effect of Concentration, pH and Time on the Morphology of Silver Nanoparticles Synthesized by Green Method using Phyllanthus Niruri and Solanum Nigrum Leaf Extracts, International Journal of Current Research and Review, 10, 21, (2018), 25-29 http://dx.doi.org/10.31782/IJCRR.2018.2529
  25. Komang Tri Aksari Dewi, Kartini Kartini, Johan Sukweenadhi, Christina Avanti, Karakter fisik dan aktivitas antibakteri nanopartikel perak hasil green synthesis menggunakan ekstrak air daun sendok (Plantago major L.), Pharmaceutical Sciences and Research (PSR), 6, 2, (2019), 69-81 https://doi.org/10.7454/psr.v6i2.4220
  26. Chris Octavianus, Imelda Hotmarisi Silalahi, Gusrizal Gusrizal, Synthesis of Silver Nanoparticles Using Premna serratifolia Linn. Leaf Extract as Reducing Agent and Their Antibacterial Activity, Jurnal Farmasi Sains dan Komunitas, 19, 1, (2022), 34-40 https://doi.org/10.24071/jpsc.003185
  27. Aparajita Verma, Mohan Singh Mehata, Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity, Journal of Radiation Research and Applied Sciences, 9, 1, (2016), 109-115 https://doi.org/10.1016/j.jrras.2015.11.001
  28. Ian L. Gunsolus, Maral P. S. Mousavi, Kadir Hussein, Philippe Bühlmann, Christy L. Haynes, Effects of Humic and Fulvic Acids on Silver Nanoparticle Stability, Dissolution, and Toxicity, Environmental Science & Technology, 49, 13, (2015), 8078-8086 https://doi.org/10.1021/acs.est.5b01496
  29. M. H. El-Rafie, M. E. El-Naggar, M. A. Ramadan, Moustafa M. G. Fouda, Salem S. Al-Deyab, A. Hebeish, Environmental synthesis of silver nanoparticles using hydroxypropyl starch and their characterization, Carbohydrate Polymers, 86, 2, (2011), 630-635 https://doi.org/10.1016/j.carbpol.2011.04.088
  30. Rizky Noviani Ridwan, Gusrizal Gusrizal, Nurlina Nurlina, Sri Juari Santosa, Sintesis dan Studi Stabilitas Nanopartikel Perak Tertudung Asam Salisilat, Indonesian Journal of Pure and Applied Chemistry, 1, 3, (2019), 83-90 https://dx.doi.org/10.26418/indonesian.v1i3.34195
  31. Heerak Chugh, Damini Sood, Ishita Chandra, Vartika Tomar, Gagan Dhawan, Ramesh Chandra, Role of gold and silver nanoparticles in cancer nano-medicine, Artificial Cells, Nanomedicine, and Biotechnology, 46, sup1, (2018), 1210-1220 https://doi.org/10.1080/21691401.2018.1449118
  32. Tiwi Prasetyaningtyas, Agung Tri Prasetya, Nuni Widiarti, Sintesis Nanopartikel Perak Termodifikasi Kitosan dengan Bioreduktor Ekstrak Daun Kemangi (Ocimum basilicum L.) dan Uji Aktivitasnya sebagai Antibakteri, Indonesian Journal of Chemical Science, 9, 1, (2020), 37-43
  33. Abdalrahim Alahmad, Armin Feldhoff, Nadja C. Bigall, Pascal Rusch, Thomas Scheper, Johanna-Gabriela Walter, Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities, Nanomaterials, 11, 2, (2021), 487 https://doi.org/10.3390/nano11020487
  34. R. M. Silverstein, F. X. Webster, D. Kiemle, Spectrometric Identification of Organic Compounds, 7th Edition, Wiley, 2005,
  35. Gusrizal Gusrizal, Sri Juari Santosa, Eko Sri Kunarti, Bambang Rusdiarso, Synthesis of Silver Nanoparticles by Reduction of Silver Ion with m-Hydroxybenzoic Acid, Asian Journal of Chemistry, 29, 7, (2017), 1417-1422 https://doi.org/10.14233/ajchem.2017.20436
  36. Syahruddin Kasim, Paulina Taba, Ruslan, Romi Anto, Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Eceng Gondok (Eichornia crassipes) Sebagai Bioreduktor, KOVALEN: Jurnal Riset Kimia, 6, 2, (2020), 126-133 https://doi.org/10.22487/kovalen.2020.v6.i2.15137
  37. Bipul Biswas, Kimberly Rogers, Fredrick McLaughlin, Dwayne Daniels, Anand Yadav, Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria, International Journal of Microbiology, 2013, (2013), 746165 https://doi.org/10.1155/2013/746165
  38. Praphulla Rao, M. S. Chandraprasad, Y. N. Lakshmi, Jahanvi Rao, P. Aishwarya, Srinidhi Shetty, Biosynthesis of silver nanoparticles using lemon extract and its antibacterial activity, International Journal of Multidisciplinary and Current Research, 2, (2014), 165-169

Last update:

No citation recorded.

Last update: 2024-06-20 18:36:35

No citation recorded.