DOI: https://doi.org/10.14710/jksa.27.12.590-596

Biodegradation of the Ciprofloxacin Antibiotic by White Rot Fungus Trametes hirsuta D7

1Master Program in Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia

3Research Center for Applied Microbiology, Research Organization of Life Science and Environment, National Research and Innovation Agency (BRIN), Cibinong, Indonesia

4 Faculty of Medicine, IPB University, Bogor, Indonesia

View all affiliations
Received: 24 Jul 2024; Revised: 30 Dec 2024; Accepted: 30 Dec 2024; Published: 31 Dec 2024.
Open Access Copyright 2024 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract

This study highlights the potential of fungal microorganisms, particularly Trametes hirsuta D7, in addressing antibiotic contaminants, such as ciprofloxacin, in the environment. The degradation process was conducted at room temperature over 7 days, and the results demonstrated that laccase was predominant in the degradation capacity; this was evidenced by the laccase enzyme activity levels obtained, namely 93 U/L, 120 U/L, and 130 U/L, compared to manganese peroxide activity of 7 U/L, 16 U/L, and 13 U/L at concentrations of 100, 300, and 500 mg/L, respectively. Notably, the laccase enzyme of T. hirsuta D7 exhibited significant degradation of ciprofloxacin, with high degradation rates of 64% at 100 mg/L, 48% at 300 mg/L, and 26% at 300 mg/L. This indicates that laccase from T. hirsuta D7 effectively degraded ciprofloxacin at various concentrations. Furthermore, this study revealed that ciprofloxacin did not significantly affect the growth of T. hirsuta D7. This suggests that microorganisms can survive and function effectively in the presence of antibiotic contamination without being impaired by these compounds. In conclusion, this study presents a potential solution for environmental antibiotic contamination by utilizing fungal microorganisms, particularly T. hirsuta D7, and their laccase enzymes. The findings of this study provide valuable insights for developing more environmentally sustainable and efficient degradation methods for antibiotic contamination in the ecosystem.

Fulltext View|Download
Keywords: Antibiotics; biodegradation; ciprofloxacin; laccase; Trametes hirsuta D7; white rot fungus
Funding: Program House of Research Organization for Life Science and Environment, Nation Research and Innovation Agency (BRIN)

Article Metrics:

Article Info
Section: Research Articles
Language : EN
Recent articles
Thermodynamic Study of Chitosan as a Corrosion Inhibitor for Carbon Steel in Chloride Solutions (NaCl and HCl) at Various Temperatures and Concentrations Green Synthesis of 4-Nitro-4’-Methoxy Chalcone by Grinding Technique and its Antibacterial Activity Cigarette Filter-Based Membranes with Tannin and FeCl₃ Additives for Enhancing the Antifouling Properties of Oil Emulsion Filtration More recent articles
Most cited articles
Skrining Metabolit Sekunder Bakteri Endofit yang Berfungsi sebagai Antidiabetes dari Daun Mimba (Azadirachta Indica) Modifikasi Zeolit Alam Menggunakan TiO2 sebagai Fotokatalis Zat Pewarna Indigo Carmine Sintesis Metil Eugenol dan Benzil Eugenol dari Minyak Daun Cengkeh Isolasi Bakteri Termofilik Sumber Air Panas Gedongsongo dengan Media Pengaya Minimal YT (Yeast Tripton) serta Identifikasi Genotipik dan Fenotipik Penggunaan Zeolit Alam Terdealuminasi Sebagai Adsorben Senyawa Aromatik More cited articles
  1. Akashdeep Singh Oberoi, Yanyan Jia, Huiqun Zhang, Samir Kumar Khanal, Hui Lu, Insights into the Fate and Removal of Antibiotics in Engineered Biological Treatment Systems: A Critical Review, Environmental Science & Technology, 53, 13, (2019), 7234-7264 https://doi.org/10.1021/acs.est.9b01131
  2. Kela P. Weber, Michael R. Mitzel, Robin M. Slawson, Raymond L. Legge, Effect of ciprofloxacin on microbiological development in wetland mesocosms, Water Research, 45, 10, (2011), 3185-3196 https://doi.org/10.1016/j.watres.2011.03.042
  3. Tanu Shree Roy, Surya Akter, Monabbir Rafsan Fahim, Md Abdul Gafur, Tahmina Ferdous, Incorporation of Ag-doped ZnO nanorod through Graphite hybridization: Effective approach for degradation of Ciprofloxacin, Heliyon, 9, 2, (2023), e13130 https://doi.org/10.1016/j.heliyon.2023.e13130
  4. Feng-Yang Bai, Shuang Ni, Yi-Zhen Tang, Xiu-Mei Pan, Zhen Zhao, Ciprofloxacin transformation in aqueous environments: Mechanism, kinetics, and toxicity assessment during •OH-mediated oxidation, Science of The Total Environment, 699, (2020), 134190 https://doi.org/10.1016/j.scitotenv.2019.134190
  5. Xi Hu, Xinjiang Hu, Qingqing Peng, Lu Zhou, Xiaofei Tan, Luhua Jiang, Chunfang Tang, Hui Wang, Shaoheng Liu, Yaqin Wang, Ziqi Ning, Mechanisms underlying the photocatalytic degradation pathway of ciprofloxacin with heterogeneous TiO2, Chemical Engineering Journal, 380, (2020), 122366 https://doi.org/10.1016/j.cej.2019.122366
  6. Daniel R. Wagner, Kevin Ament, Lina Mayr, Thomas Martin, André Bloesser, Holger Schmalz, Roland Marschall, Friedrich E. Wagner, Josef Breu, Terrestrial solar radiation driven photodecomposition of ciprofloxacin in clinical wastewater applying mesostructured iron(III) oxide, Environmental Science and Pollution Research, 28, 5, (2021), 6222-6231 https://doi.org/10.1007/s11356-020-10899-6
  7. Mohamed Hussein Abdurahman, Ahmad Zuhairi Abdullah, Noor Fazliani Shoparwe, A comprehensive review on sonocatalytic, photocatalytic, and sonophotocatalytic processes for the degradation of antibiotics in water: Synergistic mechanism and degradation pathway, Chemical Engineering Journal, 413, (2021), 127412 https://doi.org/10.1016/j.cej.2020.127412
  8. Eleni Routoula, Siddharth V. Patwardhan, Degradation of Anthraquinone Dyes from Effluents: A Review Focusing on Enzymatic Dye Degradation with Industrial Potential, Environmental Science & Technology, 54, 2, (2020), 647-664 https://doi.org/10.1021/acs.est.9b03737
  9. Chu-Wen Yang, Chien Liu, Bea-Ven Chang, Biodegradation of Amoxicillin, Tetracyclines and Sulfonamides in Wastewater Sludge, Water, 12, 8, (2020), 2147 https://doi.org/10.3390/w12082147
  10. Lanqin Yang, Benle Liu, Yuanyuan Lu, Fangyi Lu, Xiangyang Wu, Wenhua You, Biao Huang, Bioavailability of cadmium to celery (Apium graveolens L.) grown in acidic and Cd-contaminated greenhouse soil as affected by the application of hydroxyapatite with different particle sizes, Chemosphere, 240, (2020), 124916 https://doi.org/10.1016/j.chemosphere.2019.124916
  11. Nan Gao, Chun-Xiao Liu, Qiu-Man Xu, Jing-Sheng Cheng, Ying-Jin Yuan, Simultaneous removal of ciprofloxacin, norfloxacin, sulfamethoxazole by co-producing oxidative enzymes system of Phanerochaete chrysosporium and Pycnoporus sanguineu, Chemosphere, 195, (2018), 146-155 https://doi.org/10.1016/j.chemosphere.2017.12.062
  12. Agnieszka Cuprys, Paisley Thomson, Gayatri Suresh, Tarek Roussi, Satinder Kaur Brar, Patrick Drogui, Potential of agro-industrial produced laccase to remove ciprofloxacin, Environmental Science and Pollution Research, 29, 7, (2022), 10112-10121 https://doi.org/10.1007/s11356-021-13578-2
  13. Nedjwa Mansouri, Ouided Benslama, WITHDRAWN: Molecular docking exploration of the degradation activity of some synthetic hydrocarbons polymers by the laccase enzyme of Streptomyces, Materials Today: Proceedings, 54, (2022), 970-973 https://doi.org/10.1016/j.matpr.2021.12.169
  14. Xianghua Wen, Yannan Jia, Jiaxi Li, Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium, Journal of Hazardous Materials, 177, 1, (2010), 924-928 https://doi.org/10.1016/j.jhazmat.2010.01.005
  15. Caroline Aparecida Vaz de Araujo, Giselle Maria Maciel, Elidiane Andressa Rodrigues, Larissa Lachi Silva, Roselene Ferreira Oliveira, Tatiane Brugnari, Rosane Marina Peralta, Cristina Giatti Marques de Souza, Simultaneous Removal of the Antimicrobial Activity and Toxicity of Sulfamethoxazole and Trimethoprim by White Rot Fungi, Water, Air, & Soil Pollution, 228, 9, (2017), 341 https://doi.org/10.1007/s11270-017-3525-z
  16. Sushil Kumar Singh, Robinka Khajuria, Loveleen Kaur, Biodegradation of ciprofloxacin by white rot fungus Pleurotus ostreatus, 3 Biotech, 7, (2017), 69 https://doi.org/10.1007/s13205-017-0684-y
  17. Piyangkun Lueangjaroenkit, Churapa Teerapatsakul, Kazuo Sakka, Makiko Sakka, Tetsuya Kimura, Emi Kunitake, Lerluck Chitradon, Two Manganese Peroxidases and a Laccase of Trametes polyzona KU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System, Mycobiology, 47, 2, (2019), 217-229 https://doi.org/10.1080/12298093.2019.1589900
  18. A. I. Rodarte-Morales, G. Feijoo, M. T. Moreira, J. M. Lema, Degradation of selected pharmaceutical and personal care products (PPCPs) by white-rot fungi, World Journal of Microbiology and Biotechnology, 27, 8, (2011), 1839-1846 https://doi.org/10.1007/s11274-010-0642-x
  19. Rafiqul Alam, Fenny Clara Ardiati, Nissa Nurfajrin Solihat, Md Badrul Alam, Sang Han Lee, Dede Heri Yuli Yanto, Takashi Watanabe, Sunghwan Kim, Biodegradation and metabolic pathway of anthraquinone dyes by Trametes hirsuta D7 immobilized in light expanded clay aggregate and cytotoxicity assessment, Journal of Hazardous Materials, 405, (2021), 124176 https://doi.org/10.1016/j.jhazmat.2020.124176
  20. Sita Heris Anita, Fahriya Puspita Sari, Dede Heri Yuli Yanto, Decolorization of synthetic dyes by ligninolytic enzymes from Trametes hirsuta D7, Makara Journal of Science, 23, 1, (2019), 44-50
  21. Tamilvendan Manavalan, Arulmani Manavalan, Klaus Heese, Characterization of Lignocellulolytic Enzymes from White-Rot Fungi, Current Microbiology, 70, 4, (2015), 485-498 https://doi.org/10.1007/s00284-014-0743-0
  22. Alessandra Piscitelli, Paola Giardina, Vincenzo Lettera, Cinzia Pezzella, Giovanni Sannia, Vincenza Faraco, Induction and Transcriptional Regulation of Laccases in Fungi, Current Genomics, 12, 2, (2011), 104-112 http://dx.doi.org/10.2174/138920211795564331
  23. Ailette Prieto, Monika Möder, Rosario Rodil, Lorenz Adrian, Ernest Marco-Urrea, Degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products, Bioresource Technology, 102, 23, (2011), 10987-10995 https://doi.org/10.1016/j.biortech.2011.08.055
  24. Fei Zheng, Qi An, Ge Meng, Xue-Jun Wu, Yu-Cheng Dai, Jing Si, Bao-Kai Cui, A novel laccase from white rot fungus Trametes orientalis: Purification, characterization, and application, International Journal of Biological Macromolecules, 102, (2017), 758-770 https://doi.org/10.1016/j.ijbiomac.2017.04.089
  25. Marina M. Atilano-Camino, Luis H. Álvarez-Valencia, Alcione García-González, Refugio B. García-Reyes, Improving laccase production from Trametes versicolor using lignocellulosic residues as cosubstrates and evaluation of enzymes for blue wastewater biodegradation, Journal of Environmental Management, 275, (2020), 111231 https://doi.org/10.1016/j.jenvman.2020.111231
  26. Claudia M. Rivera-Hoyos, Edwin David Morales-Álvarez, Raúl A. Poutou-Piñales, Aura Marina Pedroza-Rodríguez, Refugio RodrÍguez-Vázquez, Julio M. Delgado-Boada, Fungal laccases, Fungal Biology Reviews, 27, 3, (2013), 67-82 https://doi.org/10.1016/j.fbr.2013.07.001
  27. Ayda Maadani Mallak, Amir Lakzian, Elham Khodaverdi, Gholam Hossein Haghnia, Shima Mahmoudi, Effect of Pleurotus ostreatus and Trametes versicolor on triclosan biodegradation and activity of laccase and manganese peroxidase enzymes, Microbial Pathogenesis, 149, (2020), 104473 https://doi.org/10.1016/j.micpath.2020.104473
  28. Purvi Mathur, Doyeli Sanyal, Pannalal Dey, Optimization of growth conditions for enhancing the production of microbial laccase and its application in treating antibiotic contamination in wastewater, 3 Biotech, 11, 2, (2021), 81 https://doi.org/10.1007/s13205-020-02627-1
  29. Kavitha Keshava Navada, Ananda Kulal, Enzymatic degradation of chloramphenicol by laccase from Trametes hirsuta and comparison among mediators, International Biodeterioration & Biodegradation, 138, (2019), 63-69 https://doi.org/10.1016/j.ibiod.2018.12.012
  30. Monika Čvančarová, Monika Moeder, Alena Filipová, Tomáš Cajthaml, Biotransformation of fluoroquinolone antibiotics by ligninolytic fungi – Metabolites, enzymes and residual antibacterial activity, Chemosphere, 136, (2015), 311-320 https://doi.org/10.1016/j.chemosphere.2014.12.012
  31. Rayana Manasfi, Serge Chiron, Nicola Montemurro, Sandra Perez, Monica Brienza, Biodegradation of fluoroquinolone antibiotics and the climbazole fungicide by Trichoderma species, Environmental Science and Pollution Research, 27, 18, (2020), 23331-23341 https://doi.org/10.1007/s11356-020-08442-8

Last update:

No citation recorded.

Last update: 2025-01-21 11:50:03

No citation recorded.