skip to main content

PENGHAMBATAN α-GLUCOSIDASE OLEH FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN MANGROVE Sonneratia alba SEBAGAI ANTIDIABETES MELALUI PENAMBATAN MOLEKULER

*Yunita Eka Puspitasari orcid scopus  -  Program Studi Pengolahan Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Indonesia, Indonesia
Hezkiel Oktorully Tampubolon  -  Program Studi Pengolahan Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Indonesia, Indonesia
Alifiah Nur Fajrin  -  Program Studi Pengolahan Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Indonesia, Indonesia
Titik Dwi Sulistiyati  -  Program Studi Pengolahan Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Indonesia, Indonesia
Hardoko Hardoko orcid  -  Program Studi Pengolahan Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Indonesia, Indonesia

Citation Format:
Abstract

α-glucosidase memiliki peran penting dalam metabolisme karbohidrat pada tubuh manusia dengan bekerja memutus rantai oligosakarida menjadi gula sederhana seperti monosakarida.Penghambatan aktivitas α-glucosidase menjadi salah satu mekanisme untuk mengendalikan kadar glukosa dalam darah penderita diabetes. Obat herbal sebagai inhibitor α-glucosidase bersumber dari bahan alami laut khususnya mangrove menarik perhatian. Buah pedada Sonneratia alba telah diketahui mengandung senyawa fitokimia yang berpotensi sebagai antidiabetes, akan tetapi belum pernah dilaporkan kandungan senyawa bioaktif yang berperan sebagai inhibitor α-glucosidase. Kelimpahan daun pada tanaman mangrove lebih tinggi daripada buah sehingga pada penelitian ini menggunakan daun sebagai penghambat aktivitas α-glucosidase. Tujuan dari penelitian ini adalah untuk menganalisa profil senyawa fitokimia flavonoid dari ekstrak etil asetat daun pedada S. alba, dan untuk menentukan senyawa fitokimia tersebut yang berperan sebagai inhibitor α-glucosidase secara in silico melalui penambatan molekuler. Hasil penelitian menunjukkan tiga senyawa fitokimia flavonoid dari ekstrak etil asetat daun pedada S. alba seperti luteolin, apigenin, dan diosmetin telah diidentifikasi. Menurut kaidah Lipinski, analisa druglikeness dan toksisitas, maka semua senyawa tersebut aman untuk dikonsumsi, Hasil penambatan molekuler menunjukkan energi ikatan yang dihasilkan dari interaksi reseptor dan ligan uji lebih rendah daripada energi ikatan dari reseptor dan ligan kontrol maupun alami. Sehingga dapat disimpulkan bahwa luteolin, apigenin dan diosmetin dari ekstrak etil asetat daun pedada berpotensi sebagai inhibitor α-glucosidase.

 

α-glucosidase plays an important role in carbohydrate metabolism in the human body by breakdown oligosaccharides into monosaccharides. The inhibition of α-glucosidase activity is one of the mechanisms for controlling blood glucose levels in diabetes patients. Herbal drug as an α-glucosidase inhibitor from marine resources, especially mangroves is attracting interest. The fruit of Sonneratia alba contains phytochemical compounds potent as antidiabetic but no report related to their bioactive compound as inhibitor α-glucosidase. The abundance of leaves is higher than the fruit; therefore, in this study, the leaves will be used as α-glucosidase inhibitors. This study aimed to profile bioactive compounds of ethyl acetate extract of pedada S. alba leaves and to evaluate phytochemical compounds of S. alba leaves as α-glucosidase inhibitors using molecular docking in silico. The results showed that three phytochemical compounds of ethyl acetate extract of S. alba leaves, such as luteolin, apigenin, and diosmetin have been identified. According to the Lipinski’s rule, drug likeness, and toxicity test, all compounds are safe to consume. Docking studies showed that binding energy between receptor and phytochemical compounds is lower than binding energy between receptor and ligand control. In conclusion, luteolin, apigenin and diosmetin of ethyl acetate extract of pedada S. alba leaves have potent as α-glucosidase inhibitors.

Fulltext View|Download
Keywords: Sonneratia alba; diabetes; α-glucosidase
Funding: Hibah Penelitian Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya No. 3/UN10.F06/PP/2022

Article Metrics:

  1. Alfian, S. D., Sukandar, H., Arisanti, N., & Abdulah, R. (2016). Complementary and alternative medicine use decreases adherence to prescribed medication in diabetes patients. Annals of Tropical Medicine and Public Health, 9(3), 174–179
  2. Banerjee, P., Dehnbostel, F. O., & Preissner, R. (2018). Prediction Is a Balancing Act : Importance of Sampling Methods to Balance Sensitivity and Specificity of Predictive Models Based on Imbalanced Chemical Data Sets. 6(August), 1–11. https://doi.org/10.3389/fchem.2018.00362
  3. Banerjee, P., Eckert, A. O., Schrey, A. K., & Preissner, R. (2018). ProTox-II : a webserver for the prediction of toxicity of chemicals. 46(April), 257–263. https://doi.org/10.1093/nar/gky318
  4. Bibi, S. N., Fawzi, M. M., Gokhan, Z., Rajesh, J., Nadeem, N., R, R. K. R., & Albuquerque, R. D. D. G. (2019). Ethnopharmacology, Phytochemistry, and Global Distribution of Mangroves-A Comprehensive Review. Marine Drugs, 17(231). https://doi.org/10.3390/md17040231
  5. Dona, R., Fadhli, H., Furi, M., & Viryana, T. (2021). Uji Ekstrak Etanol serta Fraksi Buah Kedabu (Sonneratia ovata Backer) Sebagai Inhibitor Enzim α-glukosidase. Jurnal Farmasi Sains Dan Praktis, 7(1), 2579–4558. http://journal.ummgl.ac.id/index.php/pharmacy
  6. Drwal, M. N., Banerjee, P., Dunkel, M., Wettig, M. R., & Preissner, R. (2014). ProTox : a web server for the in silico prediction of rodent oral toxicity. 42(May), 53–58. https://doi.org/10.1093/nar/gku401
  7. Gaspersz, N., & Sohilait, M. R. (2019). Penambatan Molekuler α , β , and γ - Mangostin Sebagai Inhibitor α -Amilase Pankreas Manusia. Indo. J. Chem. Res, 6(2), 59–66
  8. Hardoko, H., Sasmito, B. B., & Fitriani, E. N. (2020). Studi Aktivitas Antidiabet Cuka Buah Mangrove Pedada (Sonneratia alba) Secara In Vivo. Journal of Fisheries and Marine Research, 4(3), 399–407. https://doi.org/10.21776/ub.jfmr.2020.004.03.13
  9. Hardoko, Puspitasari, Y. E., & Suprayitno, E. (2015). α-glucosidase inhibitory activities of Rhizophora mucronata fruitpowder. International Journal of ChemTech Research, 8(1), 211–215
  10. Hardoko, Sasmito, B. B., & Puspitasari, Y. E. (2017). Tannin extract characterization of young mangrove Rhizophora mucronata leaves as ingredients for diabetic functional food. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 19(2), 331–336
  11. Hossain, M. K., Dayem, A. A., Han, J., Yin, Y., Kim, K., Saha, S. K., Yang, G. M., Choi, H. Y., & Cho, S. G. (2016). Molecular mechanisms of the anti-obesity and anti-diabetic properties of flavonoids. International Journal of Molecular Sciences, 17(4). https://doi.org/10.3390/ijms17040569
  12. Li, H., Song, F., Xing, J., Tsao, R., Liu, Z., & Liu, S. (2009). Screening and Structural Characterization of α-Glucosidase Inhibitors from Hawthorn Leaf Flavonoids Extract by Ultrafiltration LC-DAD-MSn and SORI-CID FTICR MS. Journal of the American Society for Mass Spectrometry, 20(8), 1496–1503. https://doi.org/10.1016/j.jasms.2009.04.003
  13. Lipinski, C. A., Lombardo, F., Dominy, B. W., & Feeney, P. J. (1997). Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Advanced Drug Delivery Reviews, 23, 3–25. https://doi.org/https://doi.org/10.1016/S0169-409X(96)00423-1
  14. Puspitasari, Y. E., Hardoko, H., Sulistiyati, T. D., Fajrin, N. A., & Tampubolon, H. O. (2022). Identifikasi Senyawa Fitokimia dari Daun Mangrove Sonneratia alba dan Analisis in Silico Sebagai Antidiabetes (Phytochemical Compound Identification of Mangrove Leaves Sonneratia alba and in Silico Analysis as Antidiabetic). Jurnal Perikanan Dan Kelautan, 27(2), 241–248. https://doi.org/10.31258/jpk.27.2.241-248
  15. Sadeghi, M., Moradi, M., Madanchi, H., & Johari, B. (2021). In silico study of garlic (Allium sativum L.)-derived compounds molecular interactions with α-glucosidase. In In Silico Pharmacology (Vol. 9, Issue 1). https://doi.org/10.1007/s40203-020-00072-9
  16. Sarkar, B. K., Barman, S. K., Akhter, S., Akter, R., Das, J., Sarkar, A. P., Akter, R., & Kundu, S. K. (2019). Evaluation of in vitro anti diabetic activity of two mangrove plant extracts : Heritiera fomes and Sonneratia apetala. Journal of Pharmacognosy and Phytochemistry, 8(6), 2376–2380
  17. Sasmito, B. B., Puspitasari, Y. E., & Hardoko, H. (2016). Antidiabetic and antioxidant activities of tannin extract of Rhizophora mucronata leaves. Journal of Chemical and Pharmaceutical Research, 8(3), 143–148
  18. Simlai, A., Rai, A., Mishra, S., Mukherjee, K., & Roy, A. (2014). Antimicrobial and antioxidative activities in the bark extracts of Sonneratia caseolaris, A mangrove plant. EXCLI Journal, 13, 997–1010
  19. Sivakumar, K., Kannappan, S., Vijayakumar, B., Jithendran, K. P., Balasubramaniam, S., & Panigrahi, A. (2021). Molecular docking study of bio-inhibitors extracted from marine macro-alga Ulva fasciata against hemolysin protein of luminescence disease-causing Vibrio harveyi. Archives of Microbiology, 203(7), 4243–4258. https://doi.org/10.1007/s00203-021-02408-1
  20. Taha, M., Alshamrani, F. J., Rahim, F., Hayat, S., Ullah, H., Zaman, K., Imran, S., Khan, K. M., & Naz, F. (2019). Synthesis of novel triazinoindole-based thiourea hybrid: A study on α-glucosidase inhibitors and their molecular docking. Molecules, 24(21). https://doi.org/10.3390/molecules24213819
  21. The International Diabetes Federation. (2021). Diabetes facts & figures. The International Diabetes Federation. https://www.idf.org/aboutdiabetes/what-is-diabetes/facts-figures.html
  22. Udrea, A. M., Gradisteanu Pircalabioru, G., Boboc, A. A., Mares, C., Dinache, A., Mernea, M., & Avram, S. (2021). Advanced bioinformatics tools in the pharmacokinetic profiles of natural and synthetic compounds with anti-diabetic activity. Biomolecules, 11(11), 1–31. https://doi.org/10.3390/biom11111692
  23. Williams, L. K., Li, C., Withers, S. G., & Brayer, G. D. (2012). Order and disorder: Differential structural impacts of myricetin and ethyl caffeate on human amylase, an antidiabetic target. Journal of Medicinal Chemistry, 55(22), 10177–10186. https://doi.org/10.1021/jm301273u
  24. Wu, L., Liu, Y., Qin, Y., Wang, L., & Wu, Z. (2019). HPLC-ESI-qTOF-MS/MS characterization, antioxidant activities and inhibitory ability of digestive enzymes with molecular docking analysis of various parts of raspberry (Rubus ideaus L.). Antioxidants, 8(8), 1–18. https://doi.org/10.3390/antiox8080274

Last update:

No citation recorded.

Last update: 2024-03-28 08:16:20

No citation recorded.