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Effect of Hydrogen Peroxide on Hydrogen Production from Melon Fruit (Cucumis melo L.) Waste by Anaerobic Digestion Microbial Community

1Biotechnology Study Program, The Graduate School, Gadjah Mada University, Yogyakarta 55281, Indonesia

2Department of Chemical Engineering, Faculty of Engineering, Gadjah Mada University, Yogyakarta 55281, Indonesia

3Department of Food Sciences and Technology Department, Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta 55281, Indonesia

Received: 25 Aug 2021; Revised: 27 Sep 2021; Accepted: 2 Oct 2021; Available online: 10 Oct 2021; Published: 1 Feb 2022.
Editor(s): Rock Keey Liew
Open Access Copyright (c) 2022 The Authors. Published by Centre of Biomass and Renewable Energy (CBIORE)
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Biohydrogen (H2) production has the potential to provide clean, environmentally friendly, and cost-effective energy sources. The effect of increasing oxidative stress on biohydrogen production by acid-treated anaerobic digestion microbial communities was studied. The use of varying amounts of hydrogen peroxide (H2O2; 0.1, 0.2, and 0.4 mM) for enhancing hydrogen production from melon fruit waste was investigated. It was found that H2O2 amendment to the H2-producing mixed culture increased hydrogen production. Treatment with 0.4 mM H2O2 increased cumulative H2 output by 7.7% (954.6 mL/L), whereas treatment with 0.1 mM H2O2 enhanced H2 yield by 23.8% (228.2 mL/gVS) compared to the untreated control. All treatments showed a high H2 production rate when the pH was 4.5 – 7.0.  H2O2-treated samples exhibited greater resilience to pH reduction and maintained their H2 production rate as the system became more acidic during H2 fermentation. The application of H2O2 affected the volatile fatty acid (VFA) profile during biohydrogen fermentation, with an increase in acetic and propionic acid and a reduction in formic acid concentration. The H2O2 treatment positively affects H2 production and is proposed as an alternative way of improving H2 fermentation.
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Keywords: Biohydrogen; dark fermentation; fruit wastes; mixed culture; oxidative pressure
Funding: LPDP

Article Metrics:

  1. Amekan, Y. and Guntoro (2017) Bioethanol production using alginate from Sargassum binderi as an immobilization matrix for Saccharomyces cerevisiae D.01 cells in a batch reactor with circulation. Research Journal of Pharmaceutical, Biological and Chemical Science. 8(2), 1925-1933
  2. Amekan, Y. (2020). The influence of microbial community dynamics on anaerobic digestion efficiency and stability: A review. International Journal of Renewable Energy Development, 9(1), 85–95.
  3. Amekan, Y., Wangi, D. S. A. P., Cahyanto, M. N., Sarto, and Widada, J. (2018). Effect of different inoculum combination on biohydrogen production from melon fruit waste. International Journal of Renewable Energy Development, 7(2), 101–109.
  4. Bundhoo, Z. M. A. (2019). Potential of bio-hydrogen production from dark fermentation of crop residues: A review. International Journal of Hydrogen Energy, 44(32), 17346–17362.
  5. Cahyari, K., Hidayat, M., Syamsiah, S., and Sarto. (2019). Optimization of hydrogen production from fruit waste through mesophilic and thermophilic dark fermentation: Effect of substrate-to-inoculum ratio. Malaysian Journal of Analytical Sciences, 23(1), 116–123.
  6. Cai, G., Jin, B., Monis, P., and Saint, C. (2011). Metabolic flux network and analysis of fermentative hydrogen production. Biotechnology Advances, 29(4), 375–387.
  7. Choi, J., and Ahn, Y. (2014). Characteristics of biohydrogen fermentation from various substrates. International Journal of Hydrogen Energy, 39(7), 3152–3159.
  8. Chu, C. Y., Sen, B., Lay, C. H., Lin, Y. C., and Lin, C. Y. (2012). Direct fermentation of sweet potato to produce maximal hydrogen and ethanol. Applied Energy, 100, 10–18.
  9. Damayanti, A., Sarto, and Sediawan, W. B. (2020). Biohydrogen production by reusing immobilized mixed culture in batch system. International Journal of Renewable Energy Development, 9(1), 37–42.
  10. Das, D., Dutta, T., Nath, K., Kotay, S. M., Das, A. K., and Veziroglu, T. N. (2006). Role of Fe-hydrogenase in biological hydrogen production. Current Science, 90(12), 1627–1637
  11. Ding, C., Yang, K. L., and He, J. (2016). Biological and fermentative production of hydrogen. In J. C. Rafael Luque, Carol Sze Ki Lin, Karen Wilson (Ed.), Processes and Technologies (Second Edi, Issue December 2016). Handbook of Biofuels Production Woodhead Publishing.
  12. Eroglu, E., and Melis, A. (2011). Photobiological hydrogen production: Recent advances and state of the art. Bioresource Technology, 102(18), 8403–8413.
  13. Hallenbeck, P. C. (2009). Fermentative hydrogen production: Principles, progress, and prognosis. International Journal of Hydrogen Energy, 34(17), 7379–7389.
  14. Hao, F., and Shao, W. (2021). What really drives the deployment of renewable energy? A global assessment of 118 countries. Energy Research and Social Science, 72(November 2020), 101880.
  15. Hassan, N. S., Jalil, A. A., Vo, D. V. N., and Nabgan, W. (2020). An overview on the efficiency of biohydrogen production from cellulose. Biomass Conversion and Biorefinery.
  16. Hawkes, F. R., Dinsdale, R., Hawkes, D. L., and Hussy, I. (2002). Sustainable fermentative hydrogen production: Challenges for process optimisation. International Journal of Hydrogen Energy, 27(11–12), 1339–1347.
  17. Hillmann, F., Fischer, R. J., Saint-Prix, F., Girbal, L., and Bahl, H. (2008). PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum. Molecular Microbiology, 68(4), 848–860.
  18. Kawasaki, S., Nakagawa, T., Nishiyama, Y., Benno, Y., Uchimura, T., Komagata, K., Kozaki, M., and Niimura, Y. (1998). Effect of oxygen on the growth of Clostridium butyricum (type species of the genus Clostridium), and the distribution of enzymes for oxygen and for active oxygen species in clostridia. Journal of Fermentation and Bioengineering, 86(4), 368–372.
  19. Khanal, S. K., Chen, W. H., Li, L., and Sung, S. (2004). Biological hydrogen production: Effects of pH and intermediate products. International Journal of Hydrogen Energy, 29(11), 1123–1131.
  20. Kim, S. H., Kumar, G., Chen, W. H., and Khanal, S. K. (2021). Renewable hydrogen production from biomass and wastes (ReBioH2-2020). Bioresource Technology, 331.
  21. Kumar, G., Sen, B., Sivagurunathan, P., and Lin, C. Y. (2015). Comparative evaluation of hydrogen fermentation of de-oiled Jatropha waste hydrolyzates. International Journal of Hydrogen Energy, 40(34), 10766–10774.
  22. Lee, H. S., Salerno, M. B., and Rittmann, B. E. (2008). Thermodynamic evaluation on H2 production in glucose fermentation. Environmental Science and Technology, 42(7), 2401–2407.
  23. Li, J., Zheng, G., He, J., Chang, S., and Qin, Z. (2009). Hydrogen-producing capability of anaerobic activated sludge in three types of fermentations in a continuous stirred-tank reactor. Biotechnology Advances, 27(5), 573–577.
  24. Luo, G., Karakashev, D., Xie, L., Zhou, Q., and Angelidaki, I. (2011). Long-term effect of inoculum pretreatment on fermentative hydrogen production by repeated batch cultivations: Homoacetogenesis and methanogenesis as competitors to hydrogen production. Biotechnology and Bioengineering, 108(8), 1816–1827.
  25. Martins, F., Felgueiras, C., Smitkova, M., and Caetano, N. (2019). Analysis of fossil fuel energy consumption and environmental impacts in european countries. Energies, 12(6), 1–11.
  26. Reddy, K., Nasr, M., Kumari, S., Kumar, S., Gupta, S. K., Enitan, A. M., and Bux, F. (2017). Biohydrogen production from sugarcane bagasse hydrolysate: effects of pH, S/X, Fe2+, and magnetite nanoparticles. Environmental Science and Pollution Research, 24(9), 8790–8804.
  27. Reungsang, A., and Sreela-or, C. (2013). Bio-hydrogen production from pineapple waste extract by anaerobic mixed cultures. Energies, 6(4), 2175–2190.
  28. Saady, N. M. C. (2013). Homoacetogenesis during hydrogen production by mixed cultures dark fermentation: Unresolved challenge. International Journal of Hydrogen Energy, 38(30), 13172–13191.
  29. Shaojie, W., Zhang, T., Bao, M., Su, H., and Xu, P. (2020). Microbial Production of Hydrogen by Mixed Culture Technologies: A Review. Biotechnology Journal, 15(1), 1–8.
  30. Sivagurunathan, P., Kumar, G., Bakonyi, P., Kim, S. H., Kobayashi, T., Xu, K. Q., Lakner, G., Tóth, G., Nemestóthy, N., and Bélafi-Bakó, K. (2016). A critical review on issues and overcoming strategies for the enhancement of dark fermentative hydrogen production in continuous systems. International Journal of Hydrogen Energy, 41(6), 3820–3836.
  31. Sivagurunathan, P., Sen, B., and Lin, C. Y. (2014a). Batch fermentative hydrogen production by enriched mixed culture: Combination strategy and their microbial composition. Journal of Bioscience and Bioengineering, 117(2), 222–228.
  32. Sivagurunathan, P., Sen, B., and Lin, C. Y. (2014b). Overcoming propionic acid inhibition of hydrogen fermentation by temperature shift strategy. International Journal of Hydrogen Energy, 39(33), 19232–19241.
  33. Tanisho, S., and Ishiwata, Y. (1995). Continuous hydrogen production from molasses by fermentation using urethane foam as a support of flocks. International Journal of Hydrogen Energy, 20(7), 541–545.
  34. Wang, X., Meng, Q., Gao, L., Jin, Z., Ge, J., Liu, C., and Xing, W. (2018). Recent progress in hydrogen production from formic acid decomposition. International Journal of Hydrogen Energy, 43(14), 7055–7071.
  35. Wei, J., Liu, Z. T., and Zhang, X. (2010). Biohydrogen production from starch wastewater and application in fuel cell. International Journal of Hydrogen Energy, 35(7), 2949–2952.
  36. Xiao, B., and Liu, J. (2009). Effects of various pretreatments on biohydrogen production from sewage sludge. Chinese Science Bulletin, 54(12), 2038–2044.

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