Isolasi Enzim L-Asparaginase dari Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) dan Uji Potensi terhadap Kultur Sel Leukemia Tipe K562

Oktarina Puspitasari; Wuryanti Wuryanti

doi:10.14710/jksa.13.2.61-65

DOI: https://doi.org/10.14710/jksa.13.2.61-65

Isolasi Enzim L-Asparaginase dari Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) dan Uji Potensi terhadap Kultur Sel Leukemia Tipe K562

Oktarina Puspitasari, Wuryanti Wuryanti

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia

Published: 1 Aug 2010.

BibTex Citation Data :

@article{JKSA15917,
    author = {Oktarina Puspitasari and Wuryanti Wuryanti},
    title = {Isolasi Enzim L-Asparaginase dari Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) dan Uji Potensi terhadap Kultur Sel Leukemia Tipe K562},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {13},
    number = {2},
    year = {2010},
    keywords = {L-Asparaginase; antikanker; temulawak; MTT; ELISA Reader},
    abstract = {Enzim L- Asparaginase  merupakan enzim yang menghidrolisis L-asparagin menjadi asam L-aspartat dan amonia. L- Asparaginase  dapat digunakan untuk mengobati penyakit kanker, yang bekerja dengan cara menghambat sintesis protein sel kanker tanpa merusak sel normal. Salah satu tumbuhan sumber L- Asparaginase  adalah temulawak ( Curcuma xanthorrhiza  Roxb.). Tujuan penelitian ini yaitu mengisolasi L- Asparaginase  dari rimpang temulawak, menentukan nilai aktivitas spesifik enzim L- Asparaginase  hasil isolasi, dan mengetahui potensi antikanker enzim L- Asparaginase  hasil isolasi terhadap sel leukemia tipe K562. Isolasi dan uji potensi enzim L- Asparaginase  dari rimpang temulawak dilakukan beberapa tahap. Tahap pertama yaitu isolasi dan pemurnian parsial enzim L- Asparaginase  dari rimpang temulawak. Tahap kedua yaitu uji aktivitas spesifik enzim L- Asparaginase  pada kondisi optimum (suhu 37°C, pH 8,6, dan waktu inkubasi 30 menit). Pada enzim dengan aktivitas spesifik tertinggi dilakukan uji antikanker terhadap sel leukemia tipe K562 dengan metode viabilitas sel dengan penambahan MTT dan pengukuran menggunakan ELISA  Reader . Enzim L- Asparaginase  dari rimpang temulawak mempunyai aktivitas spesifik sebesar 77,18 unit/mg protein pada fraksi 4 (fraksi dengan tingkat kejenuhan 60-80%). Namun fraksi 4 tersebut kurang potensial untuk aktivitas antikanker sel leukemia tipe K562 karena diperoleh LC 50  sebesar 534,89 µg/mL, sedangkan standart suatu senyawa bioaktif sebagai antikanker adalah LC 50 ≤ 20   µg/mL.},
   issn = {2597-9914},   pages = {61--65}  doi = {10.14710/jksa.13.2.61-65},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/15917}
}

Citation Format:

Abstract

Enzim L-Asparaginase merupakan enzim yang menghidrolisis L-asparagin menjadi asam L-aspartat dan amonia. L-Asparaginase dapat digunakan untuk mengobati penyakit kanker, yang bekerja dengan cara menghambat sintesis protein sel kanker tanpa merusak sel normal. Salah satu tumbuhan sumber L-Asparaginase adalah temulawak (Curcuma xanthorrhiza Roxb.). Tujuan penelitian ini yaitu mengisolasi L-Asparaginase dari rimpang temulawak, menentukan nilai aktivitas spesifik enzim L-Asparaginase hasil isolasi, dan mengetahui potensi antikanker enzim L-Asparaginase hasil isolasi terhadap sel leukemia tipe K562. Isolasi dan uji potensi enzim L-Asparaginase dari rimpang temulawak dilakukan beberapa tahap. Tahap pertama yaitu isolasi dan pemurnian parsial enzim L-Asparaginase dari rimpang temulawak. Tahap kedua yaitu uji aktivitas spesifik enzim L-Asparaginase pada kondisi optimum (suhu 37°C, pH 8,6, dan waktu inkubasi 30 menit). Pada enzim dengan aktivitas spesifik tertinggi dilakukan uji antikanker terhadap sel leukemia tipe K562 dengan metode viabilitas sel dengan penambahan MTT dan pengukuran menggunakan ELISA Reader. Enzim L-Asparaginase dari rimpang temulawak mempunyai aktivitas spesifik sebesar 77,18 unit/mg protein pada fraksi 4 (fraksi dengan tingkat kejenuhan 60-80%). Namun fraksi 4 tersebut kurang potensial untuk aktivitas antikanker sel leukemia tipe K562 karena diperoleh LC₅₀sebesar 534,89 µg/mL, sedangkan standart suatu senyawa bioaktif sebagai antikanker adalah LC₅₀≤ 20 µg/mL.

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Keywords: L-Asparaginase; antikanker; temulawak; MTT; ELISA Reader

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Section: Research Articles

Language : ID

In Vol 13, No 2 (2010): Volume 13 Issue 2 Year 2010

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