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Isolasi dan Penentuan Aktivias Spesifik Enzim Bromelin dari Buah Nanas (Ananas comosus L.)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Dec 2004.
Open Access Copyright 2004 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0/.

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Abstract
Bromelin merupakan enzim proteolitik yang terdapat pada tanaman nanas (Ananas comosus L).Telah dilakukan isolasi dan penentuan aktivitas spesifik enzim bromelin dari buah nanas.Pada penelitian ini bromelin diisolasi dengan metoda ekstraksi, lalu ditentukan aktivitas unit dengan substrat kasein dan penentuan kadar protein dengan metoda Lowry menggunakan spektrofotometer. Hasil isolasi merupakan ekstrak kasar enzim bromelin dengan aktivitas spesifik 0,521 U/mg.
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Keywords: enzim; bromelin; aktivitas spesifik

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