Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
BibTex Citation Data :
@article{JKSA3846, author = {Wuryanti Wuryanti and Damin Sumardjo and Haris Budiatna}, title = {Penentuan Aktifitas Spesifik Tirosinase dari Kentang Solanum Tuberosum}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {3}, number = {2}, year = {2000}, keywords = {Tirosinase; aktifitas spesifik}, abstract = { Tirosinase dari umbi kentang (Solanum tuberosum) telah diisolasi secara fisik dan fraksinasi dilakukan dengan (NH4)2SO4 menjadi F 1 (0-10%), F 2 (10-20%), F 3 (20-30%) dan F 4 (30-60%). Penentuan aktifitas enzim ini dengan menentukan serapan menggunakan spektrofotometer UV-Vis pada panjang gelombang 475 nm, sedang kadar protein ditentukan dengan metode Lawry. Hasil penelitian menunjujakan bahwa aktifitas spesifik tironase dari kentang adalah sebagai berikut: Ekstrak kasar 4,293u/mg, F 1 (0-10%) 5,274 u/mg, F 2 (10-20%) 5,579 u/mg, F 3 (20-30%) 5,583 u/mg dan F 4 (30-60%) 5,827 u/mg. Sebgai kesimpulan dari penelitian ini adalah bahwa fraksinasi dengan (NH 4 ) 2 SO 4 dapat dicapai hingga tingkat kemurnian 1,357 terhadap ekstrak kasar dengan spesifik aktifitas 5,827 u/mg. }, issn = {2597-9914}, pages = {52--56} doi = {10.14710/jksa.3.2.52-56}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/3846} }
Refworks Citation Data :
Tirosinase dari umbi kentang (Solanum tuberosum) telah diisolasi secara fisik dan fraksinasi dilakukan dengan (NH4)2SO4 menjadi F1 (0-10%), F2 (10-20%), F3(20-30%) dan F4 (30-60%). Penentuan aktifitas enzim ini dengan menentukan serapan menggunakan spektrofotometer UV-Vis pada panjang gelombang 475 nm, sedang kadar protein ditentukan dengan metode Lawry. Hasil penelitian menunjujakan bahwa aktifitas spesifik tironase dari kentang adalah sebagai berikut: Ekstrak kasar 4,293u/mg, F1 (0-10%) 5,274 u/mg, F2 (10-20%) 5,579 u/mg, F3 (20-30%) 5,583 u/mg dan F4 (30-60%) 5,827 u/mg. Sebgai kesimpulan dari penelitian ini adalah bahwa fraksinasi dengan (NH4)2SO4 dapat dicapai hingga tingkat kemurnian 1,357 terhadap ekstrak kasar dengan spesifik aktifitas 5,827 u/mg.
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