Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
BibTex Citation Data :
@article{JKSA3846, author = {Wuryanti Wuryanti and Damin Sumardjo and Haris Budiatna}, title = {Penentuan Aktifitas Spesifik Tirosinase dari Kentang Solanum Tuberosum}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {3}, number = {2}, year = {2000}, keywords = {Tirosinase; aktifitas spesifik}, abstract = { Tirosinase dari umbi kentang (Solanum tuberosum) telah diisolasi secara fisik dan fraksinasi dilakukan dengan (NH4)2SO4 menjadi F 1 (0-10%), F 2 (10-20%), F 3 (20-30%) dan F 4 (30-60%). Penentuan aktifitas enzim ini dengan menentukan serapan menggunakan spektrofotometer UV-Vis pada panjang gelombang 475 nm, sedang kadar protein ditentukan dengan metode Lawry. Hasil penelitian menunjujakan bahwa aktifitas spesifik tironase dari kentang adalah sebagai berikut: Ekstrak kasar 4,293u/mg, F 1 (0-10%) 5,274 u/mg, F 2 (10-20%) 5,579 u/mg, F 3 (20-30%) 5,583 u/mg dan F 4 (30-60%) 5,827 u/mg. Sebgai kesimpulan dari penelitian ini adalah bahwa fraksinasi dengan (NH 4 ) 2 SO 4 dapat dicapai hingga tingkat kemurnian 1,357 terhadap ekstrak kasar dengan spesifik aktifitas 5,827 u/mg. }, issn = {2597-9914}, pages = {52--56} doi = {10.14710/jksa.3.2.52-56}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/3846} }
Refworks Citation Data :
Tirosinase dari umbi kentang (Solanum tuberosum) telah diisolasi secara fisik dan fraksinasi dilakukan dengan (NH4)2SO4 menjadi F1 (0-10%), F2 (10-20%), F3(20-30%) dan F4 (30-60%). Penentuan aktifitas enzim ini dengan menentukan serapan menggunakan spektrofotometer UV-Vis pada panjang gelombang 475 nm, sedang kadar protein ditentukan dengan metode Lawry. Hasil penelitian menunjujakan bahwa aktifitas spesifik tironase dari kentang adalah sebagai berikut: Ekstrak kasar 4,293u/mg, F1 (0-10%) 5,274 u/mg, F2 (10-20%) 5,579 u/mg, F3 (20-30%) 5,583 u/mg dan F4 (30-60%) 5,827 u/mg. Sebgai kesimpulan dari penelitian ini adalah bahwa fraksinasi dengan (NH4)2SO4 dapat dicapai hingga tingkat kemurnian 1,357 terhadap ekstrak kasar dengan spesifik aktifitas 5,827 u/mg.
Article Metrics:
Last update:
Last update: 2024-12-27 00:46:22
As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.
Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).
Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.
The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.
Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners
JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.
Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
